Genetic feminization of pheromones and its behavioral consequences in Drosophila males

Science

Volumn 276, Issue 5318, 1997, Pages 1555-1558

  Ferveur, Jean François ^a   Savarit, Fabrice ^a   O'Kane, Cahir J ^b   Sureau, Gilles ^a   Greenspan, Ralph J ^c,d   Jallon, Jean Marc ^a  

^a UNIVERSITÉ PARIS SACLAY   (France)

^b UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^c NEW YORK UNIVERSITY   (United States)

^d NEUROSCIENCES INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PHEROMONE;

ARTICLE; BEHAVIOR; COURTSHIP; DROSOPHILA; FEMINIZATION; GENE TARGETING; PRIORITY JOURNAL; SEX DETERMINATION;

ANIMALS; DROSOPHILA MELANOGASTER; FEMALE; GENE EXPRESSION REGULATION, DEVELOPMENTAL; HOMOSEXUALITY; MALE; NUCLEAR PROTEINS; RECOMBINANT FUSION PROTEINS; SEX ATTRACTANTS; SEX CHARACTERISTICS; SEX DIFFERENTIATION; SEXUAL BEHAVIOR, ANIMAL; TRANSGENES;

EID: 0030906652     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.276.5318.1555     Document Type: Article

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28. The patterns of β-galactosidase expression were determined on whole flies and on frozen sections from 3-to 5-day-old male and female flies of PGAL4 UAS-lacZ strains, with at least 10 flies per genotype. Abdominal structures that show a reproducible GAL4 expression in male adult flies are the midgut (all strains); the oenocytes (strains A through E); the testis, the anterior ejaculatory duct, or the male accessory glands (strains A, B, D, and F; E and G showed both); the crop (C, E, F, and G); the Malpighian tubules (A, E, and G); the fat body (A and G); and the nephrocytes (D). There is also some variable and nonovertapping GAL4 expression in thoracic muscles and in neurons in the thorax and the head. LacZ expression was also detected in the salivary glands of all strains, including very weak expression in the control UAS-lacZ strain.
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40. Flies from the three control strains [55B-GAL4 as subject males; Canton-S males and shibire (shi) females as objects] were chosen for their clear behavioral phenotype (8). With the PGAL4 UAS-tra males (of strains A through E), more than 60% of the 55B-GAL4 males showed sustained wing vibration (40% with strain E), more than 50% showed licking (30% with strain E), and 20 to 50% attempted copulation (10% with strain D). With males of the four control strains, 4 to 12% of 55B-GAL4 males yielded wing vibration (20% with G-tra), less than 5% showed licking, and 0% attempted copulation. 55B-GAL4 males showed 95%, 80%, and 65% of these behaviors with target shi females.
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42. All PGAL4-tra males had male external genitalia and sex combs, except strain A, in which they were largely feminized. Abdominal pigmentation was also very feminized in A-tra males, less feminized in G-tra males, and slightly feminized in C- and E-tra males.
43. We thank A. Brand, N. Perrimon, R. F. Stocker, and G. Technau for the PGAL4 strains (A = 29B, B = 1407, C = OK72, D = 323-CyO, E = OK376, F = 55B, and G = 24B); J. Connolly, J. Keane, K. Moffat, and S. Sweeney for generating strains; J. M. Belote for antibodies to tra; G. J. Blomquist, F. M. Butterworth, J. A. Coyne, and F. Romer for comments; and M. Cobb for help with the manuscript. Supported in part by the Human Frontiers Science Program grant RG 93/94 (J.-F.F., F.S., and R.J.G.), by a fellowship from the Ministry of Education and Research (G.S.), and by grants from the Wellcome Trust (034320/Z/91/Z) and the European Union (ERBSC1*CT920790) (C.J.O'K.).