A new periplasmic protein of Escherichia coli which is synthesized in spheroplasts but not in intact cells

Journal of Bacteriology

Volumn 179, Issue 6, 1997, Pages 2073-2076

  Hagenmaier, Susanne ^a   Stierhof, York Dieter ^a   Henning, Ulf ^a  

^a MAX PLANCK INST FÜR BIOLOGIE   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CYTOPLASM; ESCHERICHIA COLI; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROTEIN SYNTHESIS; SPHEROPLAST;

EID: 0030906081     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.179.6.2073-2076.1997     Document Type: Article

References (26)

1. Allison, S. L., and A. T. Phillips. 1990. Nucleotide sequence of the gene encoding the repressor for the histidine utilization genes of Pseudomonas putida. J. Bacteriol. 172:5470-5476.
2. Birdsell, D. C., and E. H. Cota-Robles. 1967. Production and ultrastructure of lysozyme and ethylenediaminetetraacetate-lysozyme spheroplasts of Escherichia coli. J. Bacteriol. 93:427-437.
3. Casadaban, M. J. 1976. Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and Mu. J. Mol. Biol. 104:541-555.
4. Chen, E. Y., and P. H. Seeburg. 1985. Supercoil sequencing: a fast and simple method for sequencing plasmid DNA. DNA 4:165-170.
5. Chomczynski, P. 1992. One-hour downward alkaline capillary transfer for blotting of DNA and RNA. Anal. Biochem. 201:134-139.
6. Grunstein, M., and J. Wallis. 1979. Colony hybridization. Methods Enzymol. 68:379-389.
7. Hamilton, C. M., M. Aldea, B. K. Washburn, P. Babitzke, and S. R. Kushner. 1989. New method for generating deletions and gene replacements in Escherichia coli. J. Bacteriol. 171:4617-4622.
8. Hawkes, R. S., E. Niday, and J. Gordon. 1982. A dot-immunobinding assay for monoclonal and other antibodies. Anal. Biochem. 119:142-147.
9. Hawley, D. K., and W. R. McClure. 1983. Compilation and analysis of Escherichia coli promoter DNA sequences. Nucleic Acids Res. 11:2237-2355.
10. Kaufmann, A., Y.-D. Stierhof, and U. Henning. 1994. New outer membrane-associated protease of Escherichia coli K-12. J. Bacteriol. 176:359-367.
11. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685.
12. Lazzaroni, J. C. Personal communication.
13. Lazzaroni, J. C., N. Fognini-Lefebvre, and R. Portalier. 1989. Cloning of the excC and excD genes involved in the release of periplasmic proteins by Escherichia coli K-12. Mol. Gen. Genet. 218:460-464.
14. Lerner, C. G., and M. Inouye. 1990. Low copy number plasmids for regulated low-level expression of cloned genes in Escherichia coli with blue/white insert screening capability. Nucleic Acids Res. 18:4631.
15. Lu, M.-J., Y.-D. Stierhof, and U. Henning. 1993. Location and unusual membrane topology of the immunity protein of Escherichia coli phage T4. J. Virol. 67:4905-4913.
16. MacIntyre, S., B. Mutschler, and U. Henning. 1991. Requirement of the SecB chaperone for export of a non-secretory polypeptide in Escherichia coli, Mol. Gen. Genet. 227:224-228.
17. Miller, J. H. 1972. Experiments in molecular genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
18. Oliver, D., R. Cabelli, K. Dolan, and G. Jarosik. 1990. Azide-resistant mutants of Escherichia coli alter the SecA protein, an azide-sensitive component of the protein export machinery. Proc. Natl. Acad. Sci. USA 87:8227-8231.
19. Osborn, M. J., J. E. Gander, E. Parisi, and J. Carson. 1972. Mechanism of assembly of the outer membrane of Salmonella typhimurium. J. Biol. Chem. 247:3962-3972.
20. Sanger, F., S. Nicklen, and A. R. Coulson. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 76:5463-5467.
21. Schatz, P. J., and J. Beckwith. 1990. Genetic analysis of protein export in Escherichia coli. Annu. Rev. Genet. 24:215-248.
22. Southern, E. M. 1975. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98:503-517.
23. Talkuder, A. A., S. Yanai, and M. Yamada. 1994. Analysis of products of the E. coli genomic genes and regulation of their expressions: an applicable procedure for genomic analysis of other microorganisms. Biosci. Biotechnol. Biochem. 58:117-120.
24. Towbin, H., T. Staehelin, and J. Gordon. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 76:4350-4354.
25. Young, R. A., and R. W. Davis. 1983. Efficient isolation of genes by using antibody probes. Proc. Natl. Acad. Sci. USA 80:1194-1198.
26. Young, R. A., and R. W. Davis. 1983. Yeast RNA polymerase II genes: isolation with antibody probes. Science 222:778-782.