Development of HIV-1 protease expression methods using the T7 phage promotor system

Applied Microbiology and Biotechnology

Volumn 47, Issue 3, 1997, Pages 241-245

  Komai, T ^a   Ishikawa, Y ^a   Yagi, R ^a   Suzuki Sunagawa, H ^a   Nishigaki, T ^a   Handa, H ^b  

^a DAIICHI SANKYO CO   (Japan)

^b TOKYO INSTITUTE OF TECHNOLOGY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

PROTEINASE;

ARTICLE; BACTERIOPHAGE; CONTROLLED STUDY; ESCHERICHIA COLI; GENE EXPRESSION REGULATION; HUMAN IMMUNODEFICIENCY VIRUS 1; MOLECULAR CLONING; NONHUMAN; PROMOTER REGION;

ESCHERICHIA COLI; HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS 1; T7 PHAGE;

EID: 0030906020     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002530050920     Document Type: Article

References (13)

1. Hostomsky Z, Appell K, Ogden RC (1989) High-level expression of self processed HIV-1 protease in Escherichia coli using a synthetic gene. Biochem Biophys Res Commun 161: 1056-1063
2. Kohl NE, Emini EA, Schlief WA, Davis LJ, Heimbach JC, Dixon RA, Scolnick EM, Sigal IS (1988) Active human immunodeficiency virus protease is required for viral infectivity. Proc Natl Acad Sci USA 85: 4686-4690
3. Komai T, Yagi R, Suzuki-Sunagawa H, Ishikawa Y, Kasuya A, Miyamoto S, Handa H, Nishigaki T (1997) Inhibition of HIV-1 protease by oxim derivatives. Biochem Biophys Res Commun (in press)
4. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685
5. Meek TD (1992) Inhibitors of HIV-1 protease. J Enzyme Inhibit 6: 65-98
6. Moffatt BA, Studier FW (1987) T7 lysozyme inhibits transcription by T7 RNA polymerase. Cell 49: 221-227
7. Ratner L, Haseltine W, Pataraca R, Livak KJ, Starcich B, Josephs SF, Doran ER, Rafalski JA, Whitehorne EA, Baumeister K, Ivanoff L, Petteway JSR, Pearson ML, Lautenberger JA, Papas TS, Ghrayeb J, Chang NT, Gallo RC, Wong-Staal F (1985) Complete nucleotide sequence of the AIDS virus, HTLV-III. Nature 313: 277-284
8. Rizzo CJ, Korant BD (1994) Genetic approaches designed to minimize cytotoxicity of retroviral protease. Methods Enzymol 241: 16-29
9. Rosenberg AH, Lade BN, Chui DS, Lin SW, Dunn JJ, Studier FW (1987) Vectors for selective expression of cloned DNAs by T7 RNA polymerase. Gene 56: 125-135
10. Stebbins J, Debouck C (1994) Expression systems of retroviral proteases. Methods Enzymol 241: 3-16
11. Studier FW, Moffatt BA (1986) Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J Mol Biol 189: 113-130
12. Studier FW, Rosenberg AH (1981) Genetic and physical mapping of the late region of bacteriophage T7 DNA by use of cloned fragments of T7 DNA. J Mol Biol 153: 503-525
13. Studier FW, Rosenberg AH, Dunn JJ, Dubendorff JW (1990) Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol 185: 60-89