An improved enzyme-linked immunosorbent assay for whole-cell determination of methanogens in samples from anaerobic reactors

Applied and Environmental Microbiology

Volumn 63, Issue 5, 1997, Pages 2001-2006

  Sørensen, Anders Hay ^a,b   Ahring, Birgitte Kiær ^a  

^a TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

^b NATIONAL ENVIRONMENTAL RESEARCH INSTITUTE   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

ANAEROBIC DIGESTION; ARTICLE; BACTERIUM DETECTION; BIOREACTOR; ENZYME LINKED IMMUNOSORBENT ASSAY; METHANOBACTERIUM; NONHUMAN;

BACTERIA (MICROORGANISMS); METHANOBACTERIUM;

EID: 0030903377     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.63.5.2001-2006.1997     Document Type: Article

References (36)

1. Ahring, B. K., M. Sandberg, and I. Angelidaki. 1995. Volatile fatty acids as indicators of process imbalance in anaerobic digestors. Appl. Microbiol. Biotechnol. 43:559-565.
2. Ahring, B. K., J. E. Schmidt, M. Winther-Nielsen, A. J. L. Macario, and E. Conway de Macario. 1993. Effect of medium composition and sludge removal on the production, composition, and architecture of thermophilic (55°C) acetate-utilizing granules from an upflow anaerobic sludge blanket reactor. Appl. Environ. Microbiol. 59:2538-2545.
3. Ahring, B. K., and P. Westermann. 1985. Sensitivity of thermophilic methanogenic bacteria to heavy metals. Curr. Microbiol. 12:273-276.
4. Angelidaki, I., and B. K. Ahring. 1993. Thermophilic anaerobic digestion of livestock waste: the effect of ammonia. Appl. Microbiol. Biotechnol. 38:560-564.
5. Angelidaki, I., S. P. Petersen, and B. K. Ahring. 1990. Effects of lipids on thermophilic anaerobic digestion and reduction of lipid inhibition upon addition of bentonite. Appl. Microhiol. Biotechnol. 33:409-472.
6. Archer, D. B. 1984. Detection and quantitation of methanogens by enzyme-linked immunosorbent assay. Appl. Environ. Microbiol. 48:797-801.
7. Bryniok, D., and W. Trosch. 1989. ELISA techniques for the determination of methanogenic bacteria. Appl. Microhiol. Biotechnol. 32:235-242.
8. Bryniok, B., and W. Trösch. 1989. Taxonomy of methanogens by ELISA techniques. Appl. Microbiol. Biotechnol. 32:243-247.
9. Catty, D., and C. Raykundalia. 1989. ELISA and related enzyme immunoassays, p. 97-154. In D. Catty (ed.), Antibodies: a practical approach, vol. II. IRL Press, Oxford, United Kingdom.
10. Challacombe, S. J. 1988. Application of ELISA to microbiology, p. 319-342. In D. M. Kemeny and S. J. Challacombe (ed.), ELISA and other solid phase immunoassays, theoretical and practical aspects. John Wiley & Sons, Chichester, United Kingdom.
11. Conway de Macario, E., A. J. L. Macario, and M. J. Wolin. 1982. Specific antisera and immunological procedures for characterization of methanogenic bacteria. J. Bacteriol. 149:320-328.
12. Conway de Macario, E., M. J. Wolin, and A. J. L. Macario. 1982. Antibody analysis of relationships among methanogenic bacteria. J. Bacteriol. 149: 316-319.
13. Deutsche Sammlung von Mikroorganismen und Zellkulturen. 1989. Catalogue of strains 1989, 4th ed. Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Braunschweig, Germany.
14. Dolfing, J. 1988. Acetogenesis, p. 417-468. In A. J. B. Zehnder (ed.), Biology of anaerobic microorganisms. John Wiley & Sons, Inc., New York, N.Y.
15. Esser, P. 1988. Principles in adsorption to polystyrene. Nunc Bulletin (A/S Nunc, Roskilde, Denmark) 6:1-5.
16. Fuchs, G., E. Stupperich, and R. K. Thauer. 1978. Acetate assimilation and the synthesis of alanine, aspartate and glutamate in Methanobacterium thermoautotrophicum. Arch. Microbiol. 117:61-66.
17. Hendriksen, H. V., and B. K. Ahring. 1996. Combined removal of nitrate and carbon in granular sludge: substrate competition and activities. Antonie Leeuwenhoek 69:33-39.
18. Kemp, H. A., M. R. A. Morgan, and D. A. Archer. 1986. Enzyme-linked immunosorbent assay for methanogens using polyclonal and monoclonal antibodies, p. 39-49. In Anaerobic treatment: a grown-up technology. Aquatech '86, Industrial Presentations (Europe). B.V., Schiedam, The Netherlands.
19. Kemp, H. A., D. B. Archer, and M. R. A. Morgan. 1988. Enzyme-linked immunosorbent assays for the specific and sensitive quantification of Methanosarcina mazei and Methanobacterium bryantii. Appl. Environ. Microbiol. 54:1003-1008.
20. Kobayashi, H. A., E. Conway de Macario, R. S. Williams, and A. J. L. Macario. 1988. Direct characterization of methanogens in two high-rate anaerobic biological reactors. Appl. Environ. Microbiol. 54:693-698.
21. Koornneef, E., A. J. L. Macario, J. T. C. Grotenhuis, and E. Conway de Macario. 1990. Methanogens revealed immunologically in granules from five upflow anaerobic sludge blanket (UASB) bioreactors grown on different substrates. FEMS Microbiol. Ecol. 73:225-230.
22. Macario, A. J. L., and E. Conway de Macario. 1988. Quantitative immunologic analysis of the methanogenic flora of digesters reveals a considerable diversity. Appl. Environ. Microbiol. 54:79-86.
23. Macario, A. J. L., E. Conway de Macario, U. Ney, S. M. Schobert, and H. Sahm. 1989. Shifts in methanogenic subpopulations in a fixed-bed loop anaerobic bioreactor treating sulfite evaporator condensate. Appl. Environ. Microbiol. 55:1996-2001.
24. Macario, A. J. L., M. W. Peck, E. Conway de Macario, and D. P. Chynoweth. 1991. Unusual methanogenic flora of a wood-fermenting anaerobic bioreactor. J. Appl. Bacteriol. 71:31-37.
25. McInerney, M. J., and M. P. Bryant. 1980. Metabolic stages and energetics of microbial anaerobic digestion, p. 91-98. In D. A. Stafford, B. I. Wheatley, and D. E. Hughes (ed.), Anaerobic digestion. Applied Science Publishers Ltd., London, United Kingdom.
26. Poulsen, L. K., G. Ballard, and D. A. Stahl. 1993. Use of rRNA fluorescence in situ hybridization for measuring the activity of single cells in young and established biofilms. Appl. Environ. Microbiol. 59:1354-1360.
27. Raskin, L., L. K. Poulsen, D. R. Noguera, B. E. Rittmann, and D. A. Stahl. 1994. Quantification of methanogenic groups in anaerobic biological reactors by oligonucleotide probe hybridization. Appl. Environ. Microbiol. 60: 1241-1248.
28. Schmidt, J. E., A. J. L. Macario, B. K. Ahring, and E. Conway de Macario. 1992. Effect of magnesium on methanogenic subpopulations in a thermophilic acetate-degrading granular consortium. Appl. Environ. Microbiol. 58: 862-868.
29. Sørensen, A. H. 1996. Microbial characterization of methanogenic reactors. Ph.D. thesis. Technical University of Denmark, Lyngby.
30. Sørensen, A. H. Unpublished data.
31. Strayer, R. F., and J. M. Tiedje. 1978. Application of the fluorescent-antibody technique to the study of a methanogenic bacterium in lake sediments. Appl. Environ. Microhiol. 35:192-198.
32. Switzenbaum, M. S., E. Giraldo-Gomez, and R. F. Hickey. 1990. Monitoring of the anaerobic methane fermentation process. Enzyme Microb. Technol. 12:722-730.
33. Touzel, J. P., E. Conway de Macario, J. Nölling, W. M. de Vos, T. Zhilina, and A. M. Lysenko. 1992. DNA relatedness among some thermophilic members of the genus Methanobacterium: emendation of the species Methanobacterium thermoautotrophicum and rejection of Methanobacterium thermoformicicum as a synonym of Methanobacterium thermoautotrophicum. Int. J. Syst. Bacteriol. 42:408-411.
34. Visser, F. A., J. B. van Lier, A. J. L. Macario, and E. Conway de Macario. 1991. Diversity and population dynamics of methanogenic bacteria in a granular consortium. Appl. Environ. Microbiol. 57:1728-1734.
35. Ward, T. E., and J. I. Frea. 1980. Sediment distribution of methanogenic bacteria in Lake Erie and Cleveland Harbor. Appl. Environ. Microbiol. 39:597-603.
36. Zhao, Y., H. Zhang, D. R. Boone, and R. A. Mah. 1986. Isolation and characterization of a fast-growing, thermophilic Methanobacterium species. Appl. Environ. Microbiol. 52:1227-1229.