GH3 cells expressing constitutively active Gsα (Q227L) show enhanced hormone secretion and proliferation

Molecular and Cellular Endocrinology

Volumn 127, Issue 1, 1997, Pages 41-47

  Ham, J ^a   Ivan, M ^a   Wynford Thomas, D ^a   Scanlon, M F ^a  

^a CARDIFF UNIVERSITY   (United Kingdom)

Author keywords

cAMP; GH3 cells; Growth hormone; Gs mutation; Prolactin; Proliferation

Indexed keywords

BUCLADESINE; CYCLIC AMP; FORSKOLIN; GROWTH HORMONE; ISOBUTYLMETHYLXANTHINE; PROLACTIN; THYMIDINE; TRITIUM;

ANIMAL CELL; ARTICLE; CELL COUNT; CELL PROLIFERATION; CELL STRAIN GH3; DNA SYNTHESIS; GENE MUTATION; GROWTH HORMONE RELEASE; HORMONE RELEASE; HYPOPHYSIS TUMOR; NONHUMAN; PRIORITY JOURNAL; PROLACTIN RELEASE; RAT;

1-METHYL-3-ISOBUTYLXANTHINE; BLOTTING, WESTERN; BUCLADESINE; CELL COUNT; CELL DIVISION; CYCLIC AMP; ENZYME ACTIVATION; FORSKOLIN; GROWTH HORMONE; GTP-BINDING PROTEINS; HUMANS; MUTATION; PROLACTIN; THYMIDINE; TRANSFECTION; TUMOR CELLS, CULTURED;

EID: 0030902708     PISSN: 03037207     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0303-7207(96)03987-1     Document Type: Article

References (20)

1. Vallar, L., Spada, A. and Giannattasio, G. (1987) Altered Gs and adenylate cyclase activity in human GH-secreting pituitary adenomas. Nature 330, 566-567.
2. Spada, A., Vallar, L. and Faglia, G. (1992) G protein oncogenes in pituitary tumours. Trends Endocrinol. Metab. 3, 355-360
3. O'Sullivan, C., Barton, C.M., Staddon, S.L., Brown, C.L. and Lemoine, N.R. (1991) Activating point mutations in the gsp oncogene in human thyroid adenomas. Mol. Carcinog. 4, 345-349.
4. Landis, C., Masters, S.B., Spada, A., Pace, A.M., Bourne, H.R. an Vallar, L. (1989) GTPase inhibiting mutations activate the alpha gene of Gs and stimulate adenylyl cyclase in human pituitary tumours. Nature 340, 692-696
5. Dumont, J.E., Jauniaux, J.C. and Roger, P.P. (1989) The cyclicAMP-mediated stimulation of cell proliferation. TIBS 14, 67-71.
6. Pastan, I., Johnson, G.S. and Anderson, W.B. (1975) Role of cyclic nucleotides in growth control. Ann. Rev. Biochem. 44, 491-522.
7. Zachary, I., Masters, S.B. and Bourne, H.R. (1990) Increased mitogenic responsiveness of Swiss 3T3 cells expressing constitutively active Gsα. Biochem. Biophys. Res. Commun. 168, 1184-1193.
8. Muca, C. and Vallar, L. (1994) Expression of mutationally activated Gαs stimulates growth and differentiation of thyroid FRTL5 cells. Oncogene 9, 3647-3653.
9. Levis, M.J. and Bourne, H.R. (1992) Activation of the α subunit of Gs in intact cells alters its abundance, rate of degradation, and membrane avidity. J. Cell Biol. 119, 1297-1307.
10. Lotwick, H.S., Haynes, L.S. and Ham, J. (1990) Glycyl-L-glutamine stimulates the accumulation of A12 acetylcholinesterase but not of nicotinic acetylcholine receptors in quail embryonic myotubes by a cyclic AMP independent mechanism. J. Neurochem. 54, 1122-1129.
11. Brooker, G, Harper, J.F., Terasaki, W.L. and Moylan, R.D. (1979) Radioimmunoassay of cAMP and cGMP. In: Advances of Cyclic Nucleotide Research (Brooker, G., Greengard, P. and Robinson, G.A. eds.) Vol. 10 pp. 1-33, Raven Press, New York.
12. Boockfor, F.R. and Schwarz, L.K. (1988) Cultures of GH3 cells contain both single and dual hormone secretors. Endocrinology 122, 762-764.
13. Gaiddon, C., Boutillier, A.-L., Monnier, D., Mercken, L. and Loeffler, J.-P. (1994) Genomic effects of the putative oncogene Gsα. J. Biol. Chem. 269, 22663-22671.
14. Chen, J. and Iyengar, R. (1994) Suppression of Ras-induced transformation of NIH 3T3 cells by activated Gαs. Science 263, 1278-1281.
15. Edwards, C.A., Dieguez, C., Ham, J., Peters, J.R. and Scanlon, M.F. (1988) Evidence that growth hormone depletion and uncoupling of the regulatory protein of adenylate cyclase (Ns) both contribute to the desensitisation of growth hormone responses to growth hormone-releasing factor. J. Endocrinol. 116, 185-190.
16. Gaiddon, C., Mercken, L., Bancroft, C. and Loeffler, J.P. (1995) Transcriptional effects in GH3 cells of Gsα mutants associated with human pituitary tumours: Stimulation of adenosine 3′,5′-monophosphate response element-binding protein-mediated transcription and of prolactin and growth hormone promotor activity via protein kinase A. Endocrinology 136, 4331-4338.
17. Dorflinger, L.J. and Schonbrunn, A. (1983) Somatastatin inhibits basal and VIPstimulated hormone release by different mechanisms in GH pituitary cells. Endocrinology 113, 1551-1558.
18. Guild, S. and Drummond, A.H. (1983) Adenosine 3′,5′ cyclic monophosphate-dependent release of prolactin from GH3 pituitary tumor cells. Biochem. J. 216, 551-557.
19. 2+ in the regulation of hormone release from rat pituitary tumor (GH3) cells in culture. Biosci. Rep. 7, 93-105.
20. Mormede, P. and Baird, A. (1988) Estrogens, cAMP and phorbol esters modulate the prolactin response of GH3 cells to basic FGF. Endocrinology 122, 2265-2271.