Enhanced selection for homologous-recombinant embryonic stem cell clones with a neomycin phosphotransferase gene in antisense orientation

Transgenic Research

Volumn 6, Issue 1, 1997, Pages 27-35

  Skryabin, Boris V ^a   Schmauss, Claudia ^a  

^a MOUNT SINAI SCHOOL OF MEDICINE   (United States)

Author keywords

antisense RNA; embryonic stem cells; gene targeting; homologous recombination; ribozyme; Southern blots

Indexed keywords

ANIMALIA;

EID: 0030901555     PISSN: 09628819     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1018448931427     Document Type: Article

References (22)

1. Capecchi, M.R. (1989) Altering the genome by homologous recombination. Science 244, 1288-92.
2. Charron, J., Malynn, B.A., Robertson, E.J., Goff, S.P. and Alt, F.W. (1990) High-frequency disruption of the N-myc gene in embryonic stem and pre-B cell lines by homologous recombination. Mol. Cell. Biol. 10, 1799-804.
3. Doetschman, T., Maeda, N. and Smithies, O. (1988) Targeted mutation of the Hprt gene in mouse embryonic stem cells. Proc. Natl Acad. Sci. USA 85, 8583-7.
4. Fu, D., Skryabin, B.V. Brosius, J. & Robakis, N.K. (1995) Molecular cloning and characterization of the mouse dopamine D3 receptor gene: an additional intron and an mRNA variant. DNA and Cell biol. 14, 485-92.
5. Laird, P.W., Zijderveld, A., Linders, K., Rudnicki, M.A., Jaenisch, R. and Berns, A. (1991) Simplified mammalian DNA isolation procedure. Nucl. Acids Res. 19, 4293.
6. Lee, K.-P., Li, E., Huber, L.J., Lundis, S.C., Sharpe, A.M., Chao, M.V. and Jaenisch, R. (1992) Targeted mutation of the gene encoding the low affinity NGF receptor p75 leads to deficits in the peripheral sensory nervous system. Cell 69, 737-49.
7. Le Mouellic, H., Lallemand, Y. and Brulet, P. (1990) Targeted replacement of the homeobox gene Hox-3.1 by the Escherichia coli lacZ in mouse chimeric embryos. Proc. Natl Acad. Sci. (USA) 87, 4712-16.
8. Lufkin, T., Dierich, A., LeMeur, M., Mark, M. and Chambon, P. (1991) Disruption of the Hox-1.6 homeobox gene results in defects in a region corresponding to its rostral domain of expression. Cell 66, 1105-19.
9. Mansour, S.L., Thomas, K.R. and Capecchi, M.R. (1988) Disruption of the proto-oncogene int-2 in mouse embryoderived stem cells: a general strategy for targeting mutations to non-selectable genes. Nature 336, 348-52.
10. Pagano, R.R. (1990). Chi-square and other non-parametric tests. In Pagano, R.R. (ed.) Understanding Statistics, pp. 401-16. West Publishing Company.
11. Riele, H.T., Maandag, F.R. and Berns, A. (1992). Highly efficient gene targeting in embryonic stem cells through homologous recombination with isogenic DNA constructs. Proc. Natl. Acad. Sci. (USA) 89, 5128-32.
12. Robertson, H., Bradley, A., Kuehn, M and Evans, M. (1986). Germ-line transmission of genes introduced into cultured pluripotential cells by retroviral vector. Nature 323, 445-8.
13. Rudnicki, M.A., Braun, T., Hinuma, S. & Jaenisch, R. (1992) Inactivation of MyoD in mice leads to up-regulation of the myogenic HLH gene Myf-5 and results in apparently normal muscle development. Cell 71, 383-90.
14. 1B receptor. Science 652, 1875-8.
15. Schwartzberg, P.L., Robertson, E.J. and Goff, S.P. (1990) Targeted gene disruption of the endogenous c-alb locus by homologous recombination with DNA encoding a selectable fusion protein. Proc. Natl Acad. Sci. (USA) 87, 3210-14.
16. Selfridge, J., Pow, A.M., McWhir, J., Magin, T.M. and Melton, D.W. (1992) Gene targeting using a mouse HPRT minigene/HPRT-deficient embryonic stem cell system: inactivation of the mouse ERCC-1 gene. Somat. Cell. Mol. Genet. 18, 325-36.
17. Stage, T.K., Hertel, K.J. and Uhlenbeck, O.C. (1995) Inhibition of the hammerhead ribozyme by neomycin. RNA 1, 95-101.
18. Steinecke, P., Herget, T. and Schreier, P.H. (1992) Expression of a chimeric ribozyme gene results in endonucleolytic cleavage of target mRNA and a concomitant reduction of gene expression in vivo. EMBO J. 11, 1525-30.
19. Strobel, S.A. and Cech, T.R. (1995) Minor groove recognition of the conserved GU pair at the Tetrahymena ribozyme reaction site. Science 267, 675-9.
20. Thomas, K.R. and Capecchi, M.R. (1987) Site-directed mutagenesis by gene targeting in mouse embryo-derived stem cells. Cell 51, 503-12.
21. Yagi, T., Nada, S., Watanabe, N., Tamemoto, H., Kohmura, N., Ikawa, Y. and Aizawa, S. (1993) A novel negative selection for homologous recombinants using diphtheria toxin A fragment gene. Anal. Biochem. 214, 77-86.
22. 2-microglobulin gene produced by homologous recombination in embryonic stem cells. Nature 342, 435-8.