A novel technique for producing antibody-coated microprobes using a thiol-terminal silane and a heterobifunctional crosslinker

Journal of Neuroscience Methods

Volumn 71, Issue 2, 1997, Pages 163-168

  Routh, Vanessa H ^a   Heike, Cinda J ^a  

^a UNIFORMED SERVICES UNIVERSITY OF THE HEALTH SCIENCES   (United States)

Author keywords

Antibody microprobe; Central nervous system; In vivo release; Neuropeptide release; Substance P

Indexed keywords

ANTIBODY; SUBSTANCE P;

ARTICLE; CENTRAL NERVOUS SYSTEM; MICROMANIPULATION; NEUROTRANSMITTER RELEASE; PRIORITY JOURNAL;

ANIMALS; ANTIBODIES; AUTORADIOGRAPHY; CENTRAL NERVOUS SYSTEM; CROSS-LINKING REAGENTS; EPOXY RESINS; GLUTARAL; MICROELECTRODES; RATS; SILANES; SUBSTANCE P; SULFHYDRYL COMPOUNDS;

EID: 0030901281     PISSN: 01650270     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0165-0270(96)00138-0     Document Type: Article

References (17)

1. Bhatia, S.K., Shriver-Lake, L.C., Prior, K.J., Georger, J.H., Calvert, J.M., Bredehorst, R. and Ligler, F.S. (1989) Use of thiol-terminal silanes and heterobifunctional crosslinkers for immobilization of antibodies on silica surfaces. Anal. Biochem., 178: 408-413.
2. Chamow, S.M., Kogan, T.P., Peers, D.H., Hastings, R.C., Byrn, R.A. and Ashkenazi, A. (1992) Conjugation of soluble CD4 without loss of biological activity via a novel carbohydrate-directed cross-linking reagent. J. Biol. Chem., 267: 15916-15922.
3. Duggan, A.W. and Hendry, I.A. (1986) Laminar localization of the sites of release of immunoreactive substance P in the dorsal horn with antibody-coated microelectrodes. Neurosci. Lett., 68: 134-140.
4. Duggan, A.W., Hendry, I.A., Green, J.L., Morton, C.R. and Hutchinson, W.D. (1988) The preparation and use of antibody microprobes. J. Neurosci. Methods, 23: 241-247.
5. Duggan, A.W., Hope, P.J., Jarrott, B., Schaible, H.G. and Fleetwood-Walker, S.M. (1990). Release, spread and persistence of immunoreactive neurokinin A in the dorsal horn of the cat following noxious cutaneous stimulation. Studies with antibody microprobes. Neuroscience, 35: 195-202.
6. Heike, C.J., Neil, J.J., Massari, V.J. and Loewy, A.D. (1982) Substance P neurons project from the ventral medulla to the intermediolateral cell column and ventral horn in the rat. Brain Res., 243: 147-152.
7. Hendry, I.A., Morton, C.R. and Duggan, A.W. (1988) Analysis of antibody microprobe autoradiographs by computerized image processing. J. Neurosci. Methods, 23: 249-256.
8. Hope, P.J., Lang, C.W., Grubb, B.D. and Duggan, A.W. (1994) Release of immunoreactive galanin in the spinal cord of rats with ankle inflammation: studies with antibody microprobes. Neuroscience, 60: 801-807.
9. Hutchinson, W.D., Morton, C.R. and Terenius, L. (1990) Dynorphin A: in vivo release in the spinal cord of the cat. Brain Res., 32: 299-306.
10. Lodge, D., Caddy, K.W.T., Headley, P.M. and Biscol, T.J. (1974) The location of neurones with pontamine sky blue. Neuropharmacology, 13: 481-485.
11. Morton, C.R., Hutchinson, W.D., Hendry, I.A. and Duggan, A.W. (1989) Somatostatin: evidence for a role in thermal nociception. Brain Res., 31: 000-000.
12. Morton, C.R., Hutchinson, W.D. and I.A. Hendry (1991) Intraspinal release of substance P and calcitonin gene-related peptide during opiate dependence and withdrawal. Neuroscience, 43: 593-600.
13. O'Shannessey, D.J., Dobersen, M.J. and Quarles, R.H. (1984) A novel procedure for labelling immunoglobulin by conjugation to oligosaccharide moieties. Immunol. Lett., 8: 273-277.
14. Waterfall, A.H., Clarke, R.W. and Bennett, G.W. (1993) Detection of thyrotrophin releasing hormone in rat brain in vivo using novel antibody microprobes: effects of amphetamine. Neurosci. Lett., 151: 97-100.
15. Waterfall, A.H., Clarke, R.W. and Bennett, G.W. (1994) Novel methods for the preparation of antibody microprobes. J. Neurosci. Methods, 55: 41-45.
16. Weetall, H.H. (1969) Trypsin and papain covalently coupled to porous glass: preparation and characterization. Science, 166: 615-616.
17. Weetall, H.H. (1993) Affinity chromotography on inorganic support materials. In T.T. Ngo (ed), Molecular Interactions in Bioseparations, Plenum Press, New York, pp. 27-48.