The use of microcarrier-roller bottle culture for large-scale production of porcine hepatocytes

Tissue Engineering

Volumn 3, Issue 1, 1997, Pages 17-25

  Jauregui, Hugo O ^a,b   Naik, Sharda ^a   Santangini, Henry A ^a   Trenkler, Donna M ^a   Mullon, Claudy J P ^c  

^a RHODE ISLAND HOSPITAL   (United States)

^b BROWN UNIVERSITY   (United States)

^c W R GRACE AND CO CONN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COLLAGEN; CYTOCHROME P450; CYTOSKELETON PROTEIN; DEXTRAN; DIAZEPAM; DNA; KERATIN; MICROSPHERE; POLYSTYRENE; STYRENE; VIMENTIN;

ANIMAL CELL; ARTICLE; CELL DENSITY; CELL GROWTH; CELL ISOLATION; CELL PROLIFERATION; CELL STRUCTURE; DNA CONTENT; DRUG METABOLISM; IMAGE ANALYSIS; IMMUNOFLUORESCENCE; LIVER CELL CULTURE; MONOLAYER CULTURE; NONHUMAN; PRIORITY JOURNAL; SCANNING ELECTRON MICROSCOPY; SWINE;

EID: 0030899243     PISSN: 10763279     EISSN: None     Source Type: Journal    
DOI: 10.1089/ten.1997.3.17     Document Type: Article

References (30)

1. Rozga, J., Podesta, L., LePage, E., Morsiani, E., Moscioni, A.D., Hoffman, A., Sher, L., Villamil, F., Woolf, G., McGrath, J.V., Makowka, L., and Demetriou, A.A. A bioartificial liver to treat severe acute liver failure. Ann. Surg. 219, 538, 1994.
2. Sussman, N.L., Gislason, G.T., Conlin, C.A., and Kelly, J.H. The Hepatix extracorporeal liver assist device: Initial clinical experience. Artif. Organs 18, 390, 1994.
3. Nyberg, S.L., Shirabe, K., Peshwa, M.V., Sielaff, T.D., Crotty, P.L., Mann, H.J., Remmel, R.P., Payne, W.D., Hu, W.-S., and Cerra, F.B. Extracorporeal application of a gel-entrapment, bioartificial liver: Demonstration of drug metabolism and other biochemical functions. Cell Transplant. 2, 441, 1993.
4. Bader, A., Knop, E., Boker, K., Fruhauf, N., Schuttler, W., Oldhafer, K., Burkhard, R., Pichlmayr, R., and Sewing, K.-F. A novel bioreactor design for in vitro reconstruction of in vivo liver characteristics. Artif. Organs 19, 368, 1995.
5. Rozga, J., Holzman, M.D., Ro, M.-S., Griffin, D.W., Neuzil, D.F., Giorgio, T., Moscioni, A.D., and Demetriou, A.A. Development of a hybrid bioartificial liver. Ann. Surg. 217, 502, 1993.
6. Gerlach, J., Jorres, A., Trost, O., Hole, O., Vienken, J., Courtney, J.M., Gahl, G.M., and Neuhaus, P. Side effects of hybrid liver support therapy: TGF-α liberation in pigs, associated with extracorporeal bioreactors. Int. J. Artif. Organs 16, 604, 1993.
7. Dixit, V. Development of a bioartificial liver using isolated hepatocytes. Artif. Organs 18, 371, 1994.
8. Wolf, C.F.W., Gans, H., Subramanian, V.A., and McCoy, C.H. A rat model for study of bilirubin conjugation by a cultured cell/artificial capillary liver assist device. Int. J. Artif. Organs 2, 97, 1979.
9. Kelly, J., and Darlington, G. Modulation of the liver specific phenotype in the human hepatoblastoma line HEP G2. In Vitro Cell. Dev. Biol. 25, 217, 1989.
10. Sussman, N., and Kelly, J. Letters to the editor. Artif. Organs 17, 44, 1993.
11. Jauregui, H., and Muller, T. Letters to the editor. Artif. Organs 17, 44, 1993.
12. Clayton, D.F., Weiss, M., and Darnell, J.E.D. Liver-specific RNA metabolism in hepatoma cells: Variations in transcription rates and mRNA levels. Mol. Cell Biol. 5, 2633, 1985.
13. Nyberg, S.L., Remmel, R.P., Mann, H.J., Peshwa, M.V., Hu, W.-S., and Cerra, F.B. Primary hepatocytes outperform Hep G2 cells as the source of biotransformation functions in a bioartificial liver. Ann. Surg. 220, 59, 1994.
14. Morsiani, E., Rozga, J., Scott, H.C., Kong, L.B., Lebow, L.T., McGrath, M.F., Moscioni, A.D., and Demetriou, A.A. Automated large-scale production of porcine hepatocytes for bioartificial liver support. Transplant. Proc. 26, 3505, 1994.
15. Naik, S., Trenkler, D., Santangini, H., Pan, J., and Jauregui, H.O. Isolation and culture of porcine hepatocytes for artificial liver support. Cell Transplant 5, 107, 1996.
16. Kong, L.B., Chen, S., Demetriou, A.A., and Rozga, J. Matrix-induced liver cell aggregates (MILCA) for bioartificial liver use. Int. J. Artif. Organs 19, 72, 1996.
17. Lazar, A., Mann, H.J., Remmel, R.P., Shatford, R.A., Cerra, F.B., and Hu, W.-S. Extended liver-specific functions of porcine hepatocyte spheroids entrapped in collagen gel. In Vitro Cell. Dev. Biol. 31, 340, 1995.
18. Takahashi, M., Matsue, H., Matsushita, M., Sato, K., Nishikawa, M., Koike, M., Noto, H., Nakajima, Y., Uchino, J., Komai, T., and Hashimura, E. Does a porcine hepatocyte hybrid artificial liver prolong the survival time of anhepatic rabbits? ASAIO J. 38, M468, 1992.
19. Pan, J., Naik, S., Santangini, H., Trenkler, D., and Jauregui, H.O. Flow cytometric characterization of isolated porcine hepatocyte suspensions for liver support. Artif. Organs 20, 1173, 1996.
20. Jauregui, H.O., McMillan, P.N., Driscoll, J., and Naik, S. Attachment and long-term survival of adult rat hepatocytes in primary monolayer cultures: Comparison of different substrata and tissue culture media formulations. In Vitro Cell. Dev. Biol. 22, 13, 1986.
21. Adema, E., Shenk, D., Cahn, F., and Sinskey, A.J. Use of porous microcarriers in agitated cultures. BioPharm. 4, 20, 1990.
22. Cherry, R.S., and Papoutsakis, E.T. Hydrodynamic effects on cells in agitated tissue culture reactors. Bioproc. Eng. 1, 29, 1986.
23. Cherry, R.S., and Papoutsakis, E.T. Physical mechanisms of cell damage in microcarrier cell culture bioreactors. Biotechnol. Bioeng. 32, 1001, 1988.
24. Ryan, U.S., Mortara, M., and Whitaker, C. Methods for microcarrier culture of bovine pulmonary artery endothelial cells avoiding the use of enzymes. Tissue Cell 12, 619, 1980.
25. Dunn, J.C.Y., Yarmush, M.L., Koebe, H.G., and Tompkins, R.G. Hepatocyte function and extracellular matrix geometry: Long-term culture in a sandwich configuration. FASEB J. 3, 174, 1989.
26. Jauregui, H.O., Hayner, N.T., Driscoll, J.L., Williams-Holland, R., Lipsky, M.H., and Galletti, P.M. Trypan blue dye uptake and lactate dehydrogenase in adult rat hepatocytes. Freshly isolated cells, cell suspensions, and primary monolayer cultures. In Vitro Cell. Dev. Biol. 17, 1100, 1981.
27. Jauregui, H.O., Ng, S-F., Gann, L., and Waxman, D.J. Xenobiotic induction of P-450 (IIB1) and P-450c (IA1) and associated monooxygenase activities in primary cultures of adult rat hepatocytes. Xenobiotica 21, 1091, 1991.
28. Freshney, R.I. Induction of differentiation. In Freshney, R.I., ed., Culture of Animal Cells. New York: Alan R. Liss, 1987, p. 187.
29. Wu, F.J., Peshwa, M.V., Cerra, F.B., and Hu, W.-S. Entrapment of hepatocyte spheroids in a hollow fiber bioreactor as a potential bioartificial liver. Tissue Eng. l, 29, 1995.
30. Demetriou, A.A., Watanabe, F., and Rozga, J. Artificial hepatic support systems. In Boyer, J.L., and Ockner, R.O., eds. Progress in Liver Disease, Vol. 13. Philadelphia: W.B. Saunders, 1995, p. 331.