Use of polymerase chain reaction for the detection of Chlamydia trachomatis in ocular and nasopharyngeal specimens from infants with conjunctivitis

Pediatric Infectious Disease Journal

Volumn 16, Issue 3, 1997, Pages 293-297

  Hammerschlag, Margaret R ^a   Roblin, Patricia M ^a   Gelling, Maureen ^a   Tsumura, Naoki ^a   Jule, Jose E ^a   Kutlin, Andrei ^a  

^a SUNY DOWNSTATE MEDICAL CENTER   (United States)

Author keywords

Chlamydia trachomatis; neonatal conjunctivitis; Polymerase chain reaction

Indexed keywords

ADULT; ARTICLE; BACTERIUM CULTURE; BACTERIUM DETECTION; BACTERIUM IDENTIFICATION; CHLAMYDIA TRACHOMATIS; CHLAMYDIASIS; CONJUNCTIVITIS; HUMAN; INFANT; MAJOR CLINICAL STUDY; MOTHER; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; URINALYSIS;

BACTERIOLOGICAL TECHNIQUES; CHLAMYDIA INFECTIONS; CHLAMYDIA TRACHOMATIS; CONJUNCTIVITIS, INCLUSION; DISEASE TRANSMISSION, VERTICAL; EYE; FEMALE; HUMANS; INFANT; INFANT, NEWBORN; NASOPHARYNX; POLYMERASE CHAIN REACTION; PREDICTIVE VALUE OF TESTS; PREGNANCY; PREGNANCY COMPLICATIONS, INFECTIOUS; SENSITIVITY AND SPECIFICITY;

EID: 0030897801     PISSN: 08913668     EISSN: None     Source Type: Journal    
DOI: 10.1097/00006454-199703000-00008     Document Type: Article

References (13)

1. Hammerschlag MR, Roblin PM, Cummings C, Williams TH, Worku M, Howard LV. Comparison of enzyme immunoassay and culture for the diagnosis of chlamydial conjunctivitis and respiratory infections in infants. J Clin Microbiol 1987;25: 2306-8.
2. Hammerschlag MR, Roblin PM, Gelling M, Worku M. Comparison of two enzyme immunoassays to culture for the diagnosis of chlamydial conjunctivitis and respiratory infections in infants. J Clin Microbiol 1990;28:1725-7.
3. Roblin PM, Hammerschlag MR, Cummings C, Williams TH, Worku M. Comparison of two rapid microscopic methods and culture for detection of Chlamydia trachomatis in ocular and nasopharyngeal specimens from infants. J Clin Microbiol 1989;27:968-70.
4. Hammerschlag MR, Gelling M, Dumornay W, Roblin PM, Worku M. Office diagnosis of neonatal chlamydial conjunctivitis. Pediatr Infect Dis J 1991;10:540-1.
5. Rapoza PA, Quinn TC, Kiessling LA, Green A, Taylor HR. Assessment of neonatal conjunctivitis with direct immunofluorescent monoclonal antibody stain for Chlamydia. JAMA 1986;225:3369-73.
6. Kessler HH, Pierer K, Stuenzner D, Auer-Grumbach P, Haller EM, Marth E. Rapid detection of Chlamydia trachomatis in conjunctival, pharyngeal and urethral specimens with a new polymerase chain reaction assay. Sex Transm Dis 1994;21:191-5.
7. Loeffelholz MJ, Lewinski CA, Silver SR, et al. Detection of Chlamydia trachomatis in endocervical specimens by polymerase chain reaction. J Clin Microbiol 1992;30:2847-51.
8. Skulnick M, Chua R, Simor AE, et al. Use of the polymerase chain reaction for the detection of Chlamydia trachomatis in endocervical and urine specimens in an asymptomatic low-prevalence population of women. Diagn Microbiol Infect Dis 1994:195-201.
9. Jaschek G, Gaydos CA, Welsh LE, Quinn TC. Direct detection of Chlamydia trachomatis in urine specimens from symptomatic and asymptomatic men using a rapid polymerase chain reaction assay. J Clin Microbiol 1993;31:1209-12.
10. Bass CA, Jungkind DL, Silverman NS, Bondi JM. Clinical evaluation of a new polymerase chain reaction assay for detection of Chlamydia trachomatis in endocervical specimens. J Clin Microbiol 1993;31:2648-53.
11. Talley AR, Garcia-Ferrer F, Laycock KA, et al. Comparative diagnosis of neonatal chlamydial conjunctivitis by polymerase chain reaction and McCoy cell culture. Am J Ophthalmol 1994;117:50-7.
12. Gaydos CA, Crotchfelt KA, Kralian S, et al. Persistence of chlamydial DNA in urine after therapy [Abstract D79]. Program and Abstracts of the 36th Interscience Conference on Antimicrobial Agents and Chemotherapy, New Orleans, LA, September 15 to 18, 1996. Washington, DC: American Society for Microbiology, 1996.
13. An Q, Olive DM. Molecular cloning and nucleic acid sequencing of Chlamydia trachomatis 16S rKNA from patient samples lacking the cryptic plasmid. Mol Cell Probes 1994;8:429-35.