Purification and cDNA cloning of GDP-L-Fuc: N-acetyl-β-D-glucosaminide: α1-6 fucosyltransferase (α1-6 FucT) from human gastric cancer MKN45 cells

Journal of Biochemistry

Volumn 121, Issue 3, 1997, Pages 626-632

  Yanagidani, Shusaku ^a,c   Uozumi, Naofumi ^a   Ihara, Yoshito ^a   Miyoshi, Eiji ^a   Yamaguchi, Nozomi ^b   Taniguchi, Naoyuki ^a  

^a OSAKA UNIVERSITY MEDICAL SCHOOL   (Japan)

^b KYOTO PREFECTURAL UNIVERSITY OF MEDICINE   (Japan)

^c TOYOBO CO LTD   (Japan)

Author keywords

cDNA; Fucosyltransferase; Glycosyltransferase; N glycan; Purification

Indexed keywords

BRAIN ENZYME; COMPLEMENTARY DNA; EDETIC ACID; FUCOSYLTRANSFERASE; N ACETYL BETA GLUCOSAMINIDASE;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; CANCER CELL CULTURE; COLUMN CHROMATOGRAPHY; CONTROLLED STUDY; DNA LIBRARY; ENZYME ACTIVITY; ENZYME PURIFICATION; ENZYME STRUCTURE; HUMAN; HUMAN CELL; MOLECULAR CLONING; MOLECULAR WEIGHT; NUCLEOTIDE SEQUENCE; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; SEQUENCE HOMOLOGY; STOMACH CANCER; SWINE;

SUIDAE; SUS SCROFA;

EID: 0030897673     PISSN: 0021924X     EISSN: None     Source Type: Journal    
DOI: 10.1093/oxfordjournals.jbchem.a021631     Document Type: Article

References (22)

1. Varki, A. (1993) Biological roles of oligosaccharides: All of the theories are correct. Glycobiology 3, 97-130
2. Schachter, H. (1986) Biosynthetic controls that determine the branching and microheterogeneity of protein-bound oligosaccharides. Biochem. Cell Biol. 64, 163-181
3. Wilson, J.R., Williams, D., and Schachter, H. (1976) The control of glycoprotein synthesis: N-acetylglucosamine linkage to a mannose residue as a signal of L-fucose to the asparagine-linked N-acetylglucosamine residue of glycopeptide from α1-acid glycoprotein. Biochem. Biophys. Res. Commun. 72, 909-916
4. Longmore, G.D. and Schachter, H. (1982) Product-identification and substrate specificity studies of the GDP-fucose: 2-acetamido-2-deoxy-β-D-glucoside (Fuc-linked GlcNAc) 6-α-L-fucosyl-transferase in a Golgi-rich fraction from porcine liver. Carbohydr. Res. 100, 365-392
5. Kornfeld, S., Reitman, M.L., and Kornfeld, R. (1981) The carbohydrate-binding specificity of pea and lentil lectins. Fucose is an important determinant. J. Biol. Chem. 256, 6633-6640
6. Aoyagi, Y., Isemura, M., Yoshizawa, Z., Suzuki, Y., Sekine, C., Ono, T., and Ichida, F. (1985) Fucosylation of serum α-fetopro- tein in a patient with a primary hepatocellular carcinoma. Biochim. Biophys. Acta 830, 217-233
7. Aoyagi, Y., Suzuki, Y., Igarashi, A., Saitoh, M., Oguro, T., Yokota, S., Mori, T., Suda, T., Isemura, M., and Asakura, H. (1993) Carbohydrate structures of human α-fetoproteins of hepatocellular carcinomas: Presence of fucosylated and non-fucosylated biantennary glycans. Br. J. Cancer 67, 466-492
8. Ohno, N., Nishikawa, A., Kouketsu, M., Taga, H., Endo, Y., Hada, T., Higashino, K., and Taniguchi, N. (1992) Enzymatic basis of sugar structures of α-fetoproteins in hepatoma and hepatoblastoma cell lines: Correlation with α 1-6 fucosyltransferase, and N-acetylglucosaminyltransferases III and V. Int. J. Cancer 51, 315-317
9. Aoyagi, Y., Suzuki, Y., Igarashi, K., Yokota, T., Mori, S., Suda, T., Naitou, A., Isemura, M., and Asakura, H. (1993) High enhanced fucosylation of alpha-fetoproteins in patients with germ cell tumors. Cancer 72, 615-618
10. Huchinson, W.L., Du, M.-Q., Johnson, P.J., and Williams, R. (1991) Fucosyltransferase: Differentiation. plasma and tissue alteration in hepatocellular carcinomas and cirrhosis. Hepatology 13, 683-688
11. Voynow, J.A., Kaiser, R.S., Scanlin, T.F., and Glick, M.C. (1991) Purification and characterization of GDP-L-Fuc:N-acetyl-β-D-glucosaminide α 1-6 fucosyltransferase from culture human skin fibroblasts. J. Biol. Chem. 266, 21572-21577
12. Uozumi, N., Yanagidani, S., Miyoshi, E., Ihara, Y., Sakuma, T., Gao, C.-X., Teshima, T., Fujii, S., Shiba, T., and Taniguchi, N. (1996) Purification and cDNA cloning of porcine brain GDP-L-Fuc:N-acetyl-β-D-glucosaminide α 1-6 fucosyltransferase. J. Biol. Chem. 271, 27810-27817
13. Uozumi, N., Teshima, T., Yamamoto, T., Nishikawa, A., Gao, Y.-E., Miyoshi, E., Gao, C.-X., Noda, K., Islam, K.N., Ihara, Y., Fujii, S., Shiba, T., and Taniguchi, N. (1996) A fluorescent assay method for GDP-L-Fuc: N-acetyl-β-D-glucosaminide α 1-6 fucosyltransferase activity, involving high performance liquid chromatography. J. Biochem. 120, 385-392
14. Laemmli, U.K. (1970) Cleavage of structure proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685
15. Beyer, T.A., Sadler, J.E., and Hill, R.L. (1980) Purification to homogeneity of H blood group β-galactoside α1 lead to 2 fucosyltransferase from porcine submaxillary gland. J. Biol. Chem. 255, 5364-5372
16. 2-terminal signal anchor. J. Biol. Chem. 262, 17735-17743
17. Sarkar, M., Hull, E., Nishikawa, Y., Simpson, R.J., Moritz, R.L., Dunn, R., and Schachter, H. (1991) Molecular cloning and expression of cDNA encoding the enzyme that controls conversion of high-mannose to hybrid and complex N-glycans:UDP-N-acetylglucosamine: α-3-D mannoside β-1,2-N-acetylglucosaminyl-transferase I. Proc. Natl. Acad. Sci. USA 88, 234-238
18. Bendiak, B. and Schachter, H. (1987) Control of glycoprotein synthesis. Purification of UDP-N-acetylglucosamine: α-D-mannoside β1-2 N-acetylglucosaminyltransferase II from rat liver. J. Biol. Chem. 262, 5775-5783
19. Creighton, T.E. (1993) Proteins: Structures and molecular properties, 2nd. ed., pp. 17-20, W.H. Freeman and Company, New York
20. Paulson, J.C. and Colly, K. (1989) Glycosyltransferases. Structure, localization, and control of cell type-specific glycosylation. J. Biol. Chem. 264, 17615-17618
21. Field, M.C. and Wainwright, J. (1995) Molecular cloning of eukaryotic glycoproteins and glycolipid glycosyltransferase: a survey. Glycobiology 5, 463-472
22. 178 in FucT-IV. J. Biol. Chem. 270, 8145-8151