Topoisomerase II inhibition inmitosis produces numerical and structural chromosomalaberrations in human fibroblasts

Cytogenetics and Cell Genetics

Volumn 76, Issue 1-2, 1997, Pages 61-67

  Cimini, D ^a   Antoccia, A ^b   Tanzarella, C ^a,b   Degrassi, F ^a  

^a SAPIENZA UNIVERSITY OF ROME   (Italy)

^b ROMA TRE UNIVERSITY   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOCHALASIN B; DNA TOPOISOMERASE (ATP HYDROLYSING); ETOPOSIDE; GYRASE INHIBITOR; SATELLITE DNA;

ANAPHASE; ARTICLE; CENTROMERE; CHROMOSOME 1; CHROMOSOME 11; CHROMOSOME 7; CHROMOSOME LOSS; CONTROLLED STUDY; CYTOKINESIS; DNA PROBE; ENZYME INHIBITION; FIBROBLAST; HUMAN; HUMAN CELL; IN SITU HYBRIDIZATION; MICRONUCLEUS; MITOSIS; NUMERICAL CHROMOSOME ABERRATION; PRIORITY JOURNAL; SISTER CHROMATID; STRUCTURAL CHROMOSOME ABERRATION;

CELL LINE; CHROMOSOME ABERRATIONS; CHROMOSOMES; DNA PROBES; DNA TOPOISOMERASES, TYPE II; ENZYME INHIBITORS; ETOPOSIDE; FIBROBLASTS; HUMANS; IN SITU HYBRIDIZATION, FLUORESCENCE; MITOSIS;

EID: 0030895613     PISSN: 03010171     EISSN: 1424859X     Source Type: Journal    
DOI: 10.1159/000134517     Document Type: Article

References (30)

1. Antoccia A, Tanzarella C, Modesti D, Degrassi F: Cyto-kinesis-block micronucleus assay with kinetochorc detection in colchicine-treated human fibroblasts. Mutat Res 287:93-99 (1993).
2. Chen M, Beck WT: Differences in inhibition of chromosome separation and Gy arrest by DNA topo-isomerase II inhibitors mcrbarone and VM-26. Cancer Res 55:1509-1516 (1995).
3. Cimini D, Tanzarella C, Degrassi F: Effects of 5-azacy-tidine on the centromeric region of human fibroblasts studied by CREST staining and in situ hybridization on cvtokinesis-blockcd cells. Cyto-genel Cell Genet 72:219-224 (1996).
4. Clarke DJ, Johnson RT, Downes CS: Topoisontcrase I! inhibition prevents anaphase chromatid segregation in mammalian cells independently of the generation of DNA strand breaks. J Cell Sci 105:563-569 (1993).
5. Cooke HJ, Hindley J: Cloning of human satellite 111 DNA: different components are on different chromosomes. Nucl Acids Res 6:3177-3197 (1979).
6. Degrassi F, Tanzarella C: Immunofluorescenl staining of kinetochores in micronuclei: a new assay for the detection of aneuploidy. Mutat Res 203:339-345 (1988).
7. Downes CS, Mullinger AM, Johnson RT: Inhibitors of DNA topoisontcrase II prevent chromatid separation in mammalian cells but do not prevent exit from mitosis. Proc natl Acad Sci. USA 88:8895-8899 (1991).
8. Eastmond DA, Rupa DS, Chen H, Hascgawa L: Multicolor fluorescence in situ hybridization with centromeric DNA probes as a new approach to distinguish chromosome breakage from aneuploidy in interphasc cells and micronuclei, in Vig BK (ed): Chromosome Segregation and Aneuploidy. pp 377-390 (Springer-Verlag. Berlin 1993).
9. Eastmond DA, Tucker JD: Kinetochore localization in micronuclcatcd cytokinesis-blockcd Chinese hamster ovary cells: a new and rapid assay for identifying aneuploidy-inducing agents. Mutat Res 224:517-525 (1989).
10. Gorbskv GJ: Cell cycle progression and chromosome segregation in mammalian cells cultured in the presence of the topoisomerase II inhibitors ICRF-187 [(+>-1,2-bis(3.5-dioxopiperazinyl-1 -y! (propane: ADR-529] and ICRF-159 (razoxanc). Cancer Res 54:1042-1048 (1994).
11. Holloway SL: Sister chromatid separation in vivo and in vitro. Curr Opin Genet Devel 5:243-248 (1995).
12. Holm C: Coming undone: how to untangle a chromosome. Cell 77:955-957 (1994).
13. Holm C, Steams T, Botslein D: DNA topoisomerase II must act at mitosis to prevent nondisjunction and chromosome breakage. Mol Cell Biol 9:159-168 (1989).
14. Ishida R, Sato M, Narita T, Utsumi KR, Nishimoto T, Morita T, Nagata H, Andoh T: Inhibition of topoi-somerasc II by ICRF-193 induces polyploidization by uncoupling chromosome dynamics from other cell cycle events. J Cell Biol 126:1341-1351 (1994).
15. Kallio M, Lahdctie J: Analysis of micronuclei induced in mouse early spermatids by mitomycin C, vinblastine sulfate or etoposide using fluorescence in situ hybridization. Mutagenesis 8:561-567 (1993).
16. Rung AL, Sherwood SW, Shimkc RT: Cell-line specific differences in the control of cell cycle progression in the absence of mitosis. Proc natl Acad Sci. USA 87:9553-9557 (1990).
17. Li X, Nicklas RB: Mitotic forces control a cell cycle checkpoint. Nature 373:630-632 (1995).
18. t
19. Nicklas RB, Ward SC, Gorbskv GJ: Kinetochore chemistry is sensitive to tension and may link mitotic forces to a cell cycle checkpoint. J Cell Biol 130:929-939 (1995).
20. Palitti F, Degrassi F, De Salvia R, Fiore M, Tanzarella C: Inhibitors of DNA topoisomerases and chromosome aberrations, in Obe G, Natarajan AT (eds): Chromosomal Aberrations: Basic and Applied Aspects, pp 50-60 (Springer-Verlag, Berlin 1989).
21. Pommier Y, Kerrigan D, Covey JM, Kao-Shan CS, Whang-Peng J: Sister chromatid exchanges, chromosomal aberrations, and cytotoxicity produced by antitumor topoisomerase II inhibitors in sensitive (DC3F) and resistant (DC3F/9-OHE) Chinese hamster cells. Cancer Res 48:512-516 (1988).
22. Rieder CL, Schultz A, Cole R, Sluder G: Anaphase onset in vertebrate somatic cells is controlled by a checkpoint that monitors sister kinetochorc attachment lo the spindle. J Cell Biol 127:1301-1310 (1994)
23. Rowley R, Kort L: Novobiocin, nalidixie acid, etoposide, and 4'-(9-acridinvlamino)methansulfon-m-anisidide effects on G; and mitotic Chinese hamster ovarv cell progression. Cancer Res 49:4752-4757 (1989).
24. Shamu CE, Murray AW: Sister chromatid separation in frog egg extracts requires DNA topoisomerase II activity during anaphase. J Cell Biol 117:921-934 (1992)
25. Slavotinck A, Perry PE, Sumner AT: Micronuclei in neonatal lymphocytes treated with the topoisomcr-ase II inhibitors amsacrinc and etoposide. Mutat Res 319:215-222 (1993).
26. Sumner AT: The distribution of topoisomerase II on mammalian chromosomes. Chromosome Res 4:5-14 (1996).
27. Taagepera S, Rao PN, Drake FH, Gorbsky GJ: DNA topoisomerase Iia is the major chromosome protein recognized by the mitotic phosphoprotein antibody MPM-2. Proc natl Acad Sci. USA 90:8407-8411 (1993).
28. Tomkicl J, Cooke CA, Saitoh H, Bernal RL, Eamshaw WC: CENP-C is required for maintaining proper kinetochore size and for a timely transition to anaphase. J Cell Biol 125:531-545 (1994).
29. Uemura T, Ohkura H, Adachi Y, Morino K, Shiozaki K, Yattagida M: DNA topoisomerase II is required for condensation and separation of mitotic chromosomes in S. pombe. Cell 50:917-925 (1987).
30. Uemura T, Yanagida M: Mitotic spindle pulls but fails to separate chromosomes in type II DNA topoisomerase mutants: uncoordinated mitosis. EMBO J 5:1003-1010 (1986).