Cloning and expression of colonization factor antigen I (CFA/I) epitopes of enterotogenic Escherichia coli (ETEC) in Salmonella flagellin

Research in Microbiology

Volumn 148, Issue 3, 1997, Pages 217-228

  Luna, M G ^a   Martins, M M ^a   Newton, S M C ^b   Costa, S O P ^b   Almeida, D F ^a   Ferreira, L C S ^a  

^a FEDERAL UNIVERSITY OF RIO DE JANEIRO   (Brazil)

^b NONE

Author keywords

CFA; CFA I; Epitope; ETEC; Flagellin; Salmonella

Indexed keywords

EPITOPE; FLAGELLIN; OLIGONUCLEOTIDE;

ANIMAL EXPERIMENT; ANTIGENICITY; ARTICLE; BACTERIAL COLONIZATION; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; FIMBRIA; GENE EXPRESSION; MOLECULAR CLONING; MOUSE; NONHUMAN; PRIORITY JOURNAL; SALMONELLA;

ANIMALIA; BACTERIA (MICROORGANISMS); CELL FUSING AGENT VIRUS; ESCHERICHIA COLI; SALMONELLA; SALMONELLA ENTERICA SUBSP. ENTERICA SEROVAR MUENCHEN;

EID: 0030893365     PISSN: 09232508     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0923-2508(97)85242-4     Document Type: Article

References (35)

1. Acero-Reyes, J.R. & Newton, S.M.C. (1994), Expression of cholera toxin epitopes inserted in flagellin by attenuated Salmonella strains. Brazil. J. Genet., 17, 249-254.
2. Birnboim, H.C. & Doly, C. (1979), A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res., 1, 1513-1523.
3. th Nobel Conference. (Holmgren, J., Lindberg, A. & Möllby, R.) (pp. 22-32). Studentlitteratur, Lund, Sweden.
4. Black, R.E. (1990), Epidemiology of travellers' diarrhea and relative importance of various pathogens. Rev. Infect. Dis., 12, S73-S79.
5. Bühler, T., Hoschützky, H. & Jann, K. (1991), Analysis of colonization factor antigen I, an adhesin of enterotoxigenic Escherichia coli O78:H11: fimbrial morphology and location of the receptor-binding site. Infect. Immun., 59, 3876-3882.
6. Cassels, F.J., Deal, C.D., Reid, R.H., Jarboe, D.L., Nauss, J.L., Carter, J.M. & Boedeker, E.G. (1992), Analysis of Escherichia coli colonization factor antigen I linear B-cell epitopes, as determined by primate responses, following protein sequence verification. Infect. Immun., 60, 2174-2181.
7. Evans, D.G., Evans, D.J., Jr., Clegg, S. & Pauley, J.A. (1979), Purification and characterization of the CFA/I antigen of enterotoxigenic Escherichia coli. Infect. Immun., 25, 738-748.
8. Evans, D.G., Evans, D.J., Jr., Tjoa, W.S. & Dupont, H.L. (1978), Detection and characterization of colonization factor of enterotoxigenic Escherichia coli isolated from adults with diarrhea. Infect. Immun., 21, 727-736.
9. Evans, D.G., Graham, D.Y., Evans, D.J., Jr. & Opekun, A. (1984), Administration of purified colonization factor antigens (CFA/I, CFA/H) of enterotoxigenic Escherichia coli to volunteers. Response to challenge with virulent enterotoxigenic Escherichia coli. Gastroenterology, 87, 934-940.
10. Gothefors, L., Åhrén, C., Stoll, B., Barua, D.K., Ørskov, F., Salek, M.A. & Svennerholm, A.-M. (1985), Presence of colonization factor antigens on fresh isolates of fecal Escherichia coli: a prospective study. J. Infect. Dis., 152, 1128-1133.
11. Hall, R.H., Maneval, D.R., Collins, J.H., Theibert, J.L. & Levine, M.M. (1989), Purification and analysis of colonization factor antigen I, coli surface antigen 1, and coli surface antigen 3 fimbriae from enterotoxigenic Escherichia coli. J. Bacterial., 171, 6372-6374.
12. Ibrahim, G.F., Fleet, G.H., Lyons, M.J. & Walker, R.A. (1985), Method for the isolation of highly purified Salmonella flagellins. J. Clin. Microbiol., 22, 1040-1044.
13. Klemm, P. (1982), Primary structure of the CFA/I fimbrial protein from human enterotoxigenic Escherichia coli strains. Eur. J. Biochem., 124, 339-348.
14. Klemm, P. & Mikkelsen, L. (1982), Prediction of antigenic determinants and secondary structures of the K88 and CFA1 fimbrial proteins from enteropathogenic Escherichia coli. Infect Immun., 38, 41-45.
15. Laemmli, U.K. (1970), Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (Lond.), 227, 680-685.
16. Lopez-Vidal, Y., Klemm, P. & Svennerholm, A.-M. (1988), Monoclonal antibodies against different epitopes on colonization factor antigen I of enterotoxin-producing Escherichia coli. J. Clin. Microbiol., 26, 1967-1972.
17. Lowry, O.H., Rosebrough, N.J., Farr, A.L. & Randall, R.J. (1951), Protein measurement with the Folin phenol reagent. J. Biol. Chem., 193, 265-275.
18. Lu, Z., Murray, K.S., Cleave, V.V., LaVallie, E.R., Stahl, M.L. & McCoy, J.M. (1995), Expression of thioredoxin random peptide libraries on the Escherichia coli cell surface as functional fusions to flagellin : A system designed for exploration of protein-protein interactions. BioTechnology, 13, 366-372.
19. Maniatis, T., Fritsch, E.F. & Sambrook, J. (1982), Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, New York.
20. McConnell, M.M., Chart, H. & Rowe, B. (1989), Antigenic homology within human enterotoxigenic Escherichia coli fimbrial colonization factor antigens : CFA/I, coli-surface-associated antigens CS1, CS2, CS4 and CS17. FEMS Microbiology Lett., 61, 105-108.
21. McEwen, J., Levi, R., Horwitz, R.J. & Arnon, R. (1992), Synthetic recombinant vaccine expressing influenza haemagglutinin epitope in Salmonella flagellin leads to partial protection in mice. Vaccine, 10, 405-411.
22. Moi, F.R. & Graaf, F.K. (1985), Molecular biology of fimbriae of enterotoxigenic Escherichia coli. Curr. Top. Microbiol. Immunol., 118, 119-137.
23. Newton, S.M.C., Jacob, C.O. & Stocker, B.A.D. (1989), Immune response to cholera toxin epitope inserted in Salmonella flagellin. Science, 244, 70-72.
24. Newton, S.M.C., Joys, T.M., Anderson, S.A., Kennedy, R.C., Hovi, M.E. & Stocker, B.A.D. (1995), Expression and immunogenicity of an 18-residue epitope of HIV gp41 inserted in the flagellar protein of a Salmonella live vaccine. Res. Microbiol., 146, 203-216.
25. Newton, S.M.C., Kotb, M., Poirier, T.P., Stocker, B.A.D. & Beachey, E.H. (1991), Expression and immunogenicity of a streptococcal M protein epitope inserted in Salmonella flagellin. Infect. Immun., 59, 2158-2165.
26. Payne, D.W., Shuttleworth, A.D. & Lambert, P.A. (1991), Evaluation of a small-scale method for the extraction of the K88 antigen from enterotoxigenic Escherichia coli. FEMS Microbiol. Lett., 78, 81-84.
27. Rudin, A., McConnell, M.M. & Svennerholm, A.-M. (1994), Monoclonal antibodies against enterotoxigenic Escherichia coli colonization factor antigen (CFA/I) that cross-react immunologically with heterologous CFAs. Infect. Immun., 62, 4339-4346.
28. Rudin, A. & Svennerholm, A-M. (1996), Identification of a cross-protective linear epitope in enterotoxigenic Escherichia coli colonization factor antigen I (CFA/I) and characterization of anti-CFA/I peptide antisera. Infect. Immun. (in press).
29. Sanderson, K.E. & Stocker, B.A.D. (1987), Salmonella typhimurium strains used in genetic analysis, in "Escherichia coli and Salmonella typhimurium. Cellular and molecular biology" (Ingraham, J.L., Low, B., Magasanik, B., Schaechter, M. & Umbarger, H.E.) (pp. 1220-1224). American Society for Microbiology, Washington, D.C.
30. Sommerfeit, H., Grewal, H.M.S., Svennerholm, A.-M., Gastra, W., Flood, P.R., Viboud, G. & Bhan, M.K. (1992), Genetic relationship of putative colonization factor 0166 to colonization factor antigen I and coli surface antigen 4 of enterotoxigenic Escherichia coli. Infect. Immun., 60, 3799-3806.
31. Towbin, H. & Gordon, N. (1984), Immunoblotting and dot immunobinding - current status and outlook. J. Immunol. Methods, 72, 313-40.
32. Verma, N.K., Ziegler, H.K., Stocker, B.A.D. & Schoolnik, G.K. (1995b), Induction of a cellular immune response to a defined T-cell epitope as an insert in the flagellin of a live vaccine strain of Salmonella. Vaccine, 13, 235-244.
33. Verma, N.K., Ziegler, H.K., Wilson, M., Khan, M., Safley, S., Stocker, B.A.D. & Schoolnik, G.K. (1995a), Delivery of class I and class II MHC-restricted T-cell epitopes of listeriolysin of Listeria monocytogenes by attenuated Salmonella. Vaccine, 13, 142-150.
34. Wu, J.Y., Newton, S., Judd, A., Stocker, B. & Robinson, W.S. (1989), Expression of immunogenic epitopes of hepatitis B surface antigen with hybrid flagellin proteins by a vaccine strain of Salmonella. Proc. Natl. Acad. Sci. USA, 86, 4726-4730.
35. Yoshioka, K., Aizawa, S.-I. & Yamaguchi, S. (1995), Flagellar filament structure and cell motility of Salmonella typhimurium mutants lacking part of the outer domain of flagellin. J. Bacterial., 177, 1090-1093.