A nested polymerase chain reaction for detection of Legionella pneumophila in clinical specimens

Clinical Microbiology and Infection

Volumn 3, Issue 1, 1997, Pages 95-101

  Bernander, Sverker ^a   Hanson, Hanna Stina ^a   Johansson, Bo ^a   Von Stedingk, Lars Victor ^a  

^a KAROLINSKA UNIVERSITY HOSPITAL   (Sweden)

Author keywords

Legionella; Legionnaires' disease; Nested primers; Polymerase chain reaction (PCR)

Indexed keywords

BACTERIAL DNA; PRIMER DNA;

ARTICLE; ASPIRATION; BACTERIUM DETECTION; CLINICAL ARTICLE; CLINICAL TRIAL; CONTROLLED CLINICAL TRIAL; CONTROLLED STUDY; DIAGNOSTIC TEST; HUMAN; LEGIONELLA PNEUMOPHILA; LEGIONNAIRE DISEASE; LUNG LAVAGE; NONHUMAN; PNEUMONIA; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SPUTUM ANALYSIS;

BACTERIA (MICROORGANISMS); LEGIONELLA; LEGIONELLA PNEUMOPHILA;

EID: 0030891568     PISSN: 1198743X     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1469-0691.1997.tb00257.x     Document Type: Article

References (23)

1. Starnbach MN, Falkow S, Tomkins LS. Species-specific detection of Legionella pneumophila in water by DNA amplification. J Clin Microbiol 1989; 27: 1257-61.
2. Bej AK, Mahubani MH, Miller J, DiCesare JL, Haff L, Atlas RM. Multiplex PCR amplification and immobilized capture probes for detection of bacterial pathogens and indicators in water. Mol Cell Probes 1990; 4: 353-65
3. Jaulhac B, Nowicki M, Bornstein N, et al Detection of Legionella spp in bronchoalveolar lavage fluids by DNA amplification. J Clin Microbiol 1992; 30: 920-4.
4. Kessler HH, Reinthaler FF, Pschaid A, et al. Rapid detection of Legionella species in bronchoalveolar lavage fluids with the EnviroAmp Legionella PCR Amplification and Detection Kit. J Clin Microbiol 1993; 31: 3325-8.
5. Matsiota-Bernard P, Pitsouni E, Legakis N, Nauciel C. Evaluation of commercial amplification kit for detection of Legionella pneumophila in clinical specimens. J Clin Microbiol 1994; 32: 1503-5.
6. Lisby G, Dessau R. Construction of a DNA amplification assay for detection of Legionella species in clinical samples. Eur J Microbiol Infect Dis 1994; 13: 225-31.
7. Uldum S. Udvikling af metoder til akut-fase diagnostik af Legionella infektioner. PhD thesis Neisseriaafdelningen, Statens Seruminstitut, Copenhagen. 1994.
8. Jonas D, Rosenbaum A, Weyrich S, Bhakdi S. Enzymelinked immunoassay for detection of PCR-amplified DNA of Legionella in bronchoalveolar fluid. J Clin Microbiol 1995; 33: 1247-52.
9. Lindsay DSJ, Abraham WH, Fallon RJ. Detection of mip gene by PCR for diagnosis of Legionnaires' disease. J Clin Microbiol 1994; 32: 3068-9.
10. Maiwald M, Schill M, Stockinger C, et al. Detection of Legionella DNA in human and guinea pig urine samples by the polymerase chain reaction. Eur J Clin Microbiol Infect Dis 1995; 14: 25-33.
11. Plikaytis BB, Gelbee RH, Skinnich TM. Rapid and sensitive detection of Mycobacterium leprae using a nested-primer gene amplification assay. J Clin Microbiol 1990; 28: 1913-17.
12. Rosa PA, Hogan D, Schwan TG. Polymerase chain reaction analyses identify two distinct classes of Borrelia burgdorferi. J Clin Microbiol 1991; 29: 524-32.
13. Aurelius E, Johansson B, Sköldenberg B, Staland Å, Forsgren M. Rapid diagnosis of herpes simplex encephalitis by nested polymerase chain reaction assay of cerebrospinal fluid. Lancet 1991; 337: 189-92.
14. von Stedingk LV, Olsson I, Hanson H-S, Åsbrink E, Hovmark A. Polymerase chain reaction for detection of Borrelia burgdorferi DNA in skin lesions of early and late lyme borreliosis. Eur J Clin Microbiol Infect Dis 1995; 14: 1-5.
15. Koide M, Saito A. Diagnosis of Legionella pneumophila infection by polymerase chain reaction. Clin Infect Dis 1995; 21: 199-201.
16. Schlenk R, Wildfeuer A, Haferkamp O. Identification of Legionella pneumophila in various specimens by the polymerase chain reaction. Arzneimittelforschung 1993; 43: 1249-52.
17. Catalan V, Moreno C, Dasi MA, Munos C, Apraiz D. Nested polymerase chain reaction for detection of Legionella in water. Res Microbiol 1994; 145: 603-10.
18. Nordström K, Kallings I, Dahnsjö H, Clemens F. An out-break of Legionnaires' disease in Sweden: report of sixtyeight cases. Scand J Infect Dis 1983; 15: 43-55.
19. Edelstein PH. Improved semiselective medium for isolation of Legionella pneumophila from contaminated clinical and environmental specimens. J Clin Microbiol 1981; 14: 298-303
20. Engleberg NC, Carter C, Weber DR, Cianciotto NP, Eisenstein B. DNA-sequence of mip, a Legionella pneumophila gene, associated with macrophage infectivity. Infect Immun 1989; 57: 1263-70.
21. Mahubani MH, Bej AK, Miller R, Haff L, DiCesare J, Atlas RM. Detection of Legionella with polymerase chain reaction and gene probe methods. Mol Cell Probes 1990; 4: 175-87.
22. Edelstein PH. Legionnaires' disease. Clin Infect Dis 1993; 16: 741-9.
23. Plouffe JF, File TM Jr, Breiman RF, et al. Reevaluation of the definition of Legionnaires' disease: use of the urinary antigen assay. Clin Infect Dis 1995; 20: 1286-91.