Separation of DNA strands facilitates detection of point mutations by PCR-SSCP

BioTechniques

Volumn 22, Issue 4, 1997, Pages 604-606

  Selvakumar, Nagamiah ^a   Ding, Bei Chuan ^b   Wilson, Stuart M ^c  

^a NATIONAL INSTITUTE FOR RESEARCH IN TUBERCULOSIS   (India)

^b Beijing Res Inst for TB Control   (China)

^c Dulwich Community Hospital   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

DOUBLE STRANDED DNA; ETHIDIUM BROMIDE; RIFAMPICIN; SILVER; SINGLE STRANDED DNA;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL GENE; DNA DENATURATION; MYCOBACTERIUM TUBERCULOSIS; POLYMERASE CHAIN REACTION; PROTEIN SECONDARY STRUCTURE; SINGLE STRAND CONFORMATION POLYMORPHISM;

EID: 0030891308     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/97224bm05     Document Type: Article

References (4)

1. Orita, M., H. Iwahana, H. Kanazawa, K. Hayashi and T. Sekiya. 1989. Detection of polymorphisms of human DNA by gel electrophoresis as single-strand conformation polymorphisms. Proc. Natl. Acad. Sci. USA 86:2766-2770.
2. Sammons, D.W., L.D. Adams and E.E. Nishizawa. 1981. Ultra sensitive silver-based color staining of polypeptides in polyacrylamide gels. Electrophoresis 2:135-141.
3. Telenti, A., P. Imboden, F. Marchesi, D. Lowrie, S. Cole, M.J. Colston, L. Matter, K. Schopfer and T. Bodmer. 1993. Detection of rifampicin-resistance mutations in Mycobacterium tuberculosis. Lancet 341:647-650.
4. Telenti, A., P. Imboden, F. Marchesi, T. Schmidheini and T. Bodmer. 1993. Direct, automated detection of rifampicin-resistance by polymerase chain reaction and single strand conformation polymorphism analysis. Antimicrob. Agents Chemother. 37:2054-2058.