Semiquantitative RT-PCR: Enhancement of assay accuracy and reproducibility

BioTechniques

Volumn 22, Issue 4, 1997, Pages 630-636

  Šarić, Tomo ^a   Shain, Sydney A ^a  

^a UNIVERSITY OF TEXAS HEALTH SCIENCE CENTER AT SAN ANTONIO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; FIBROBLAST GROWTH FACTOR 1; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE;

ACCURACY; ARTICLE; DNA TEMPLATE; POLYACRYLAMIDE GEL ELECTROPHORESIS; REPRODUCIBILITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SEQUENCE ANALYSIS;

EID: 0030891157     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/97224bm12     Document Type: Article

References (6)

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2. Innis, M.A., D.H. Gelfand and J.J. Sninsky. 1995. PCR Strategies. Academic Press, San Diego.
3. Kinoshita, T., J. Imamura, H. Nagai and K. Shimotohno. 1992. Quantification of gene expression over a wide range by the polymerase chain reaction. Anal. Biochem. 206:231-235.
4. Mullis, K.B., F. Ferrer and R.A. Gibbs. 1994. The Polymerase Chain Reaction. Birkhauser, Boston.
5. Shain, S.A. and J.D. Koger. 1989. Differences in responsiveness of clonally derived AXC/SSh rat prostate cancer cells to secreted or prototypic mitogens. Cancer Res. 49:3898-3903.
6. Shain, S.A., T. Šarić, L.D. Ke, D. Nannen and S. Yoas. 1996. Endogenous fibroblast growth factor-1 or fibroblast growth factor-2 modulate prostate cancer cell proliferation. Cell Growth Differ. 7:573-586.