A rapid and nonisotopic method for the screening and sequencing of p53 gene mutations in formalin-fixed paraffin-embedded tumors

Modern Pathology

Volumn 10, Issue 3, 1997, Pages 252-258

  Soong, Richie ^a   Iacopetta, Barry J ^a  

^a UNIVERSITY OF WESTERN AUSTRALIA   (Australia)

Author keywords

Mutation detection; Nonisotopic SSCP; p53; Paraffin embedded

Indexed keywords

PROTEIN P53;

ARTICLE; CANCER; COLORECTAL CANCER; GENE MUTATION; HUMAN; HUMAN TISSUE; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SINGLE STRAND CONFORMATION POLYMORPHISM;

CARCINOMA; COLORECTAL NEOPLASMS; DNA MUTATIONAL ANALYSIS; GENES, P53; HUMANS; MUTATION; PARAFFIN EMBEDDING; POLYMERASE CHAIN REACTION; POLYMORPHISM, SINGLE-STRANDED CONFORMATIONAL; TISSUE FIXATION;

EID: 0030890851     PISSN: 08933952     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (26)

1. Harris CC, Hollstein M. Clinical implications of the p53 tumor-suppressor gene. N Engl J Med 1993;329:1318-27.
2. Bosari S, Viale G. The clinical significance of p53 aberrations in human tumors. Virchows Arch 1995;427:229-41.
3. Wynford-Thomas D. p53 in tumour pathology: can we trust immunocytochemistry? J Pathol 1992;166:329-30.
4. Dowell SP, Hall PA. The p53 tumour suppressor gene and tumour prognosis: is there a relationship? J Pathol 1995;177: 221-24.
5. Lowe SW, Ruley HE, Jacks T, Housman DE. p53-dependent apoptosis modulates the cytotoxicity of anticancer agents. Cell 1993;74:957-67.
6. Lowe SW, Bodis S, McClatchey A, Remington L, Ruley HE, Fisher DE, et al. p53 status and the efficacy of cancer therapy in vivo. Science 1994;266:807-10.
7. Bergh J, Norberg T, Sjogren S, Lindgren A, Holmberg L. Complete sequencing of the p53 gene provides prognostic information in breast cancer patients, particularly in relation to adjuvant systemic therapy and radiotherapy. Nat Med 1995;1:1029-34.
8. Goh HS, Yao J, Smith D. p53 point mutation and survival in colorectal cancer patients. Cancer Res 1995;55:5217-21.
9. Iacopetta B, DiGrandi S, Dix B, Haig C, Soong R, House A. Loss of heterozygosity of tumour suppressor gene loci in human colorectal carcinoma. Eur J Cancer 1994;5:664-70.
10. Kwok S, Higuchi R. Avoiding false positives with PCR. Nature 1989;339:237-8.
11. Bassam BJ, Caetano-Anolles G, Gresshoff PM. Fast and sensitive silver staining of DNA in polyacrylamide gels. Anal Biochem 1991;196:80-3.
12. Dix B, Robbins P, Carrello S, House A, Iacopetta B. Comparison of p53 gene mutation and protein overexpression in colorectal carcinomas. Br J Cancer 1994;70:585-90.
13. Hara F, Suzuki T, Saito M, Hikino T, Fukushima T, Oyama T, et al. Nonisotopic single-strand conformation polymorphism (PCR-SSCP) with subsequent direct sequencing after silver staining. Path Int 1994;44:815-6.
14. Jackson DP, Lewis FA, Taylor GR, Boylston AW, Quirke P. Tissue extraction of DNA and RNA and analysis by the polymerase chain reaction. J Clin Pathol 1990;43:499-504.
15. Rogers BB, Alpert LC, Hine EAS, Buffone GJ. Analysis of DNA in fresh and fixed tissue by the polymerase chain reaction. Am J Pathol 1990;136:541-8.
16. Greer CE, Peterson SL, Kiviat NB, Manos MM. PCR amplification from paraffin-embedded tissues: effects of fixative and fixative time. Am J Clin Pathol 1991;95:117-24.
17. Oto M, Miyake S, Yuasa Y. Optimization of nonradioisotopic single-strand conformation polymorphism analysis with a conventional minislab gel electrophoresis apparatus. Anal Biochem 1993;213:19-22.
18. Bosari S, Marchetti A, Buttitta F, Graziani D, Borsani G, Loda M, et al. Detection of p53 mutations by single-strand conformation polymorphism (SSCP) gel electrophoresis. Diagn Mol Pathol 1995;4:249-55.
19. Sarkar FH, Valdivieso M, Borders J, Yao K, Raval MMT, Madan SK, et al. A universal method for the mutational analysis of K-ras and p53 gene in non-small-cell lung cancer using formalin-fixed paraffin-embedded tissue. Diagn Mol Pathol 1995;4:266-73.
20. Hartmann A, Blaszyk H, McGovern RM, Schroeder JJ, Cunningham J, de Vries EMG, et al. p53 gene mutations inside and outside of exons 5-8: the patterns differ in breast and other cancers. Oncogene 1995;10:681-8.
21. Soussi T, Legros Y, Lubin R, Ory K, Schlichtholz B. Multifactorial analysis of p53 alteration in human cancer: a review. Int J Cancer 1994;57:1-9.
22. Hayashi K, Yandell DW. How sensitive is PCR-SSCP? Hum Mutat 1993;2:338-46.
23. Moyret C, Theillet C, Puig PL, Moles J, Thomas G, Hamelin R. Relative efficiency of denaturing gradient gel electrophoresis and single-strand conformation polymorphism in the detection of mutations in exons 5 to 8 of the p53 gene. Oncogene 1994;9:1739-43.
24. Ory K, Legros Y, Auguin C, Soussi T. Analysis of the most representative tumor-derived mutants reveals that changes in protein conformation are not correlated with loss of transactivation or inhibition of cell proliferation. EMBO J 1994; 13:3496-504.
25. Havely O, Michalovitz D, Oren M. Different tumor-derived p53 mutants exhibit distinct biological activities. Science 1990;250:113-6.
26. Cheng J, Haas M. Sensitivity of detection of heterozygous point mutations in p53 cDNAs by direct PCR sequencing. PCR Meth Appl 1992;1:199-201.