Overexpression and purification of human calcineurin α from Escherichia coli and assessment of catalytic functions of residues surrounding the binuclear metal center

Biochemistry

Volumn 36, Issue 16, 1997, Pages 4934-4942

  Mondragon, Angelo ^a   Griffith, Eric C ^a   Sun, Luo ^a   Xiong, Fei ^a   Armstrong, Christopher ^a   Liu, Jun O ^a  

^a MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CALCINEURIN; PHOSPHOPROTEIN PHOSPHATASE; T LYMPHOCYTE RECEPTOR; TACROLIMUS;

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; ARTICLE; CATALYSIS; CRYSTAL STRUCTURE; ENZYME ACTIVATION; ENZYME PURIFICATION; ENZYME SUBSTRATE; EUKARYOTE; GENE EXPRESSION; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SIGNAL TRANSDUCTION;

ARGININE; BINDING SITES; CALCINEURIN; CALMODULIN-BINDING PROTEINS; CHROMATOGRAPHY, AFFINITY; CLONING, MOLECULAR; ESCHERICHIA COLI; HISTIDINE; HUMANS; MODELS, MOLECULAR; MUTAGENESIS; PHOSPHOPROTEIN PHOSPHATASE; RECOMBINANT PROTEINS; SIGNAL TRANSDUCTION; TACROLIMUS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; EUKARYOTA;

EID: 0030890757     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi9631935     Document Type: Article

References (58)

1. Aperia, A., Ibarra, F., Svensson, L.-B., Klee, C., & Greengard, P. (1992) Proc. Natl. Acad. Sci. U.S.A. 89, 7394-7397.
2. Breuder, T., Hemenway, C. S., Movva, N. R., Cardenas, M. E., & Heitman, J. (1994) Proc. Natl. Acad. Sci. U.S.A. 91, 5372-5376.
3. Cardenas, M., Lorenz, M., Hemenway, C. S., & Heitman, J. (1994) Perspect. Drug Discovery Des. 2, 103-126.
4. Clipstone, N. A., & Crabtree, G. R. (1992) Nature 357, 695-697.
5. Cohen, P., & Cohen, P. T. W. (1989) J. Biol. Chem. 264, 21436-21438.
6. Cohen, P. T. W., & Cohen, P. (1989) Biochem. J. 260, 931-934.
7. Crabtree, G. R., & Clipstone, N. A. (1994) Annu. Rev. Biochem. 63, 1045-1083.
8. Cunningham, K. W., & Fink, G. R. (1994) J. Cell Biol. 124, 351-363.
9. Cyert, M. S., Kunisaaw, R., Kaim, D., & Thorner, J. (1991) Proc. Natl. Acad. Sci. U.S.A. 88, 7376-7380.
10. Dumont, F. J., Staruch, M. J., Koprak, S. L., Siekierka, J. J., Lin, C. S., Harrison, R., Sewell, T., Kindt, V. M., Beattie, T. R., Wyvratt, M., & Sigal, N. H. (1992) J. Exp. Med. 176, 751-760.
11. Duronio, R. J., Rudnick, D. A., Johnson, R. L., Linder, M. E., & Gordon, J. I. (1990) Methods: Companion Methods Enzymol. 1, 253-263.
12. Foor, F., Parent, S. A., Morin, N., Dahl, A. M., Ramadan, N., Chrebet, G., Bostian, K. A., & Nielsen, J. B. (1992) Nature 360, 682-684.
13. Friedman, J., & Weissman, I. (1991) Cell 66, 799-806.
14. Furukawa, T., Itoh, M., Krueger, N. X., Streuli, M., & Saito, H. (1994) Proc. Natl. Acad. Sci. U.S.A. 91, 10928-10932.
15. Goldberg, J., Huang, H.-B., Kwon, Y.-G., Greenbard, P., Nairn, A. C., & Kuriyan, J. (1995) Nature 376, 745-753.
16. Griffith, J. P., Kim, J. L., Kim, E. E., Sintchak, M. D., Thomson, J. A., Fitzgibbon, M. J., Fleming, M. A., Caron, P. R., Hsiao, K., & Navia, M. A. (1995) Cell 82, 507-522.
17. Guerini, D., & Klee, C. B. (1989) Proc. Natl. Acad. Sci. U.S.A. 86, 9183-9187.
18. Haddy, A., & Rusnak, F. (1994) Biochem. Biophys. Res. Commun. 200, 1221-1229.
19. Hashimoto, Y., Perrino, B. A., & Soderling, T. R. (1990) J. Biol. Chem. 265, 1924-1927.
20. Herbst, R., Carroll, P. M., Allard, J. D., Schilling, J., Raabe, T., & Simon, M. A. (1996) Cell 85, 899-909.
21. Hubbard, M. J., & Klee, C. B. (1989) Biochemistry 28, 1868-1874.
22. Kennedy, M. T., Brockman, H., & Rusnak, F. (1996) J. Biol. Chem. 271, 26517-26521.
23. Kincaid, R. L., Nightingale, M. S., & Martin, B. M. (1988) Proc. Natl. Acad. Sci. U.S.A. 85, 8983-8987.
24. King, M. M., & Huang, C. Y. (1984) J. Biol. Chem. 259, 8847-8856.
25. Kissinger, C. R., Parge, H. E., Knighton, D. R., Lewis, C. T., Pelletier, L. A., Tempczyk, A., Kalish, V. J., Tucker, K. D., Showalter, R. E., Moomaw, E. W., Gastinel, L. N., Habuka, N., Chen, X., Maldonado, F., Barker, J. E., Bacquet, R., & Villafranca, J. E. (1995) Nature 378, 641-644.
26. Klee, C. B., & Cohen, P. (1988) Adv. Second Messenger Phosphoprotein Res. 225-247.
27. Lea, J. P., Sands, J. M., McMahon, S. J., & Tumlin, J. A. (1994) Kidney Int. 46, 647-652.
28. Lewis, C., Gastinel, L., Habuka, N., Tucker, K., Chen, X., Maldonado, F., & Villafranca, J. E. (1995) FASEB J. 9, A1346.
29. Liu, J. (1993) Immunol. Today 14, 290-296.
30. Liu, J., Farmer, J. D., Lane, W. S., Friedman, J., Weissman, I., & Schreiber, S. L. (1991a) Cell 66, 807-815.
31. Liu, J., Chen, C.-M., & Walsh, C. T. (1991b) Biochemistry 30, 2306-2310.
32. Liu, J., Albers, M. W., Wandless, T. J., Luan, S., Alberg, D. G., Belshaw, P. J., Cohen, P., MacKintosh, C., Klee, C. B., & Schreiber, S. L. (1992) Biochemistry 31, 3896-3901.
33. Loh, C., Shaw, K. T.-Y., Carew, J., Viola, J. P. B., Luo, C., Perrino, B. A. & Rao, A. (1996) J. Biol. Chem. 271, 10884-10891.
34. Lohse, D. L., Denue, J. E., & Dixon, J. E. (1995) Structure 3, 987-990.
35. Martin, B. L., & Graves, D. J. (1986) J. Biol. Chem. 261, 14545-14550.
36. Mueller, E. G., Crowder, M. W., Averill, B. A., & Knowles, J. R. (1993) J. Am. Chem. Soc. 115, 2974-2975.
37. Nelson, P. A., Akselband, Y., Kawamura, A., Su, M., Tung, R. D., Rich, D. H., Kishore, V., Rosborough, S. L., DeCenzo, M. T., Livingston, D. J., & Harding, M. W. (1993) J. Immunol. 150, 2139-2147.
38. O'Keefe, S. J., Tamura, J., Kincaid, R. L., Tocci, M. J., & O'Neill, E. A. (1992) Nature 357, 692-694.
39. Parent, S. A., Nielsen, J. B., Morin, N., Chrebet, G., Ramadan, N., Dahl, A. M., Hsu, M.-J., Bostian, K. A., & Foor, F. (1993) J. Gen. Microbiol. 139, 2973-2984.
40. Perrino, B. A., Fong, Y.-L., Brickey, D. A., Saitoh, Y., Ushio, Y., Fukunaga, K., Miyamoto, E., & Soderling, T. R. (1992) J. Biol. Chem. 267, 15965-15969.
41. Perrino, B. A., Ng, L. Y., & Soderling, T. R. (1995) J. Biol. Chem. 270, 340-346.
42. Qin, K.-F., Khangulov, S., Liu, C., & Huang, C. Y. (1995) FASEB J. 9, A1347.
43. Sarker, G., & Sommer, S. S. (1990) BioTechniques 8, 404-407.
44. Schreiber, S. L., & Crabtree, G. R. (1992) Immunol. Today 13, 136-142.
45. Shenolikar, S. (1994) Annu. Rev. Cell Biol. 10, 55-86.
46. Shibasaki, F., Price, E. R., Milan, D., & McKeon, F. (1996) Nature 382, 370-373.
47. Sikkink, R., Haddy, A., MacKelvie, S., Mertz, P., Litwiller, R., & Rusnak, F. (1995) Biochemistry 34, 8348-8356.
48. Sodeoka M., Larson, C. J., Chen, L., LeClair, K. P., & Verdine, G. L. (1993) Bioorg. Med. Chem. Lett. 3, 1089-1094.
49. Stemmer, P., & Klee, C. B. (1991) Curr. Opin. Neurobiol. 1, 53-64.
50. Steamer, P. M., & Klee, C. B. (1994) Biochemistry 33, 6859-6866.
51. Sun, H., Charles, C. H., Lau, L. F., & Tonks, N. K. (1993) Cell 75, 487-493.
52. Wang, X., Culotta, V. C., & Klee, C. B. (1996) Nature 383, 434-437.
53. Webb, M. R. (1992) Proc. Natl. Acad. Sci. U.S.A. 89, 4884-4887.
54. Wesselborg, S., Fruman, D. A. Sagoo, J. K., Biere, B. E., & Burakoff, S. J. (1996) J. Biol. Chem. 271, 1274-1277.
55. Yu, L., Haddy, A., & Rusnak, F. (1995) J. Am. Chem. Soc. 117, 10147-10148.
56. Zhu, D., Cardenas, M. E., & Heitman, J. (1995) J. Biol. Chem. 270, 24831-24838.
57. Zhuo, S., Clemens, J. C., Hakes, D. J., Barford, D., & Dixon, J. E. (1993) J. Biol. Chem. 268, 17754-17761.
58. Zhuo, S., Clemens, J. C., Stone, R. L., & Dixon, J. E. (1994) J. Biol. Chem. 269, 26234-26238.