Use of bioluminescence for monitoring the viability of individual Pseudomonas putida KT2442 cells

FEMS Microbiology Letters

Volumn 149, Issue 1, 1997, Pages 133-140

  Eberl, Leo ^a,b   Givskov, Michael ^a   Poulsen, Lars Kongsbak ^a,c   Molin, Søren ^a  

^a TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

^b TECHNICAL UNIVERSITY OF MUNICH   (Germany)

^c NOVO NORDISK A S   (Denmark)

Author keywords

Cold stress; Pseudomons putida; Single cell bioluminescence

Indexed keywords

LUCIFERASE;

ARTICLE; BACTERIUM CULTURE; BIOLUMINESCENCE; CELL VIABILITY; COLD STRESS; CONTROLLED STUDY; NONHUMAN; PRIORITY JOURNAL; PSEUDOMONAS PUTIDA;

EID: 0030890091     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(97)00070-0     Document Type: Article

References (22)

1. Givskov, M., Eberl, L. and Molin, S. (1994) Responses to nutrient starvation in Pseudomonas putida strain KT2442: two-dimensional electrophoretic analysis of starvation and stress inducible proteins. J. Bacteriol. 176, 4816-4824.
2. Givskov, M., Eberl, L., Møller, S., Poulsen, L.K. and Molin, S. (1994) Response to nutrient starvation in Pseudomonas putida KT2442: analysis of general cross-protection, cell shape, and macromolecular content. J. Bacteriol. 176, 7-14.
3. Bagdasarian, M., Lurz, B., Ruckert, B., Franklin, F.C.H., Bagdasarian, M.M., Frey, J. and Timmis, K.N. (1981) Specific-purpose plasmid cloning vectors. II. Broad host range, high copy number, RSF1010-derived vectors for gene cloning in Pseudomonas. Gene 16, 237-247.
4. Clark, J.D. and Maaløe, O. (1967) DNA replication and the cell division cycle in Escherichia coli. J. Mol. Biol. 23, 99-112.
5. Bertani, G. (1951) Studies on lysogenesis, I. The mode of phage liberation by lysogenic Escherichia coli. J. Bacteriol. 62, 293-300.
6. Kogure, K., Simidu, U. and Taga, N. (1979) A tentative direct microscopic method for counting living marine bacteria. Can. J. Microbiol. 25, 415-420.
7. Kristensen, C.S., Eberl, L., Sanches-Romero, J.M., Givskov, M., Molin, S. and De Lorenzo, V. (1995) Site-specific deletions of chromosomally located DNA segments with the multimer resolution system of broad-host-range plasmid RP4. J. Bacteriol. 177, 52-58.
8. Grimberg, J., Maguire, S. and Belluscio, L. (1989) A simple method for the preparation of plasmid and chromosomal E. coli DNA. Nucleic Acids Res. 17, 8893.
9. Stahl, D.A. and Amann, R.I. (1991) Development and application of nucleic acid probes. In : Nucleic Acid Techniques in Bacterial Systematics (Stackebrandt, E. and Goodfellow, M., Eds.), pp. 205-248. Wiley, New York.
10. Møller, S., Kristensen, C.S., Poulsen, L.K., Carstensen, J.M. and Molin, S. (1995) Bacterial growth on surfaces: automated Image Analysis for quantification of growth rate-related parameters. Appl. Environ. Microbiol. 61, 741-748.
11. Davis, B.D., Luger, S.M. and Tai, P.C. (1986) Role of ribosome degradation in the death of starved Escherichia coli cells. J. Bacteriol. 166, 439-445.
12. Stewart, G.S.A.B. and Williams, P. (1992) lux genes and the application of bacterial bioluminescence. J. Gen. Microbiol. 138, 1289-1300.
13. Ferguson, Y., Glover, L.A., McGillivray, D.M. and Prosser, J.I. (1995) Survival and activity of lux-marked Aeromonas salmonicida in seawater. Appl. Environ. Microbiol. 61, 3494-3498.
14. Prosser, J.I. (1994) Molecular marker systems for the detection of genetically modified microorganisms in the environment. Microbiol. 140, 5-17.
15. Elhai, J. and Wolk, C.P. (1990) Developmental regulation and spatial pattern of expression of the structural genes for nitrogenase in the cyanobacterium Anabea. EMBO J. 9, 3379-3388.
16. Hill, P., Eberl, L., Molin, S. and Stewart, G.S.A.B. (1994) Imaging bioluminescence in single bacteria. In: Bioluminescence and Chemoluminescence (Campbell, A. K., Kricka, L.J. and Stanley, P.E., Eds.), pp. 629-632. Wiley, New York.
17. Silcock, D.J., Waterhouse, R.N., Glover, L.A., Prosser, J.I. and Killham, K. (1992) Detection of a single genetically modified bacterial cell in soil by using charge coupled device-enhanced microscopy. Appl. Environ. Microbiol. 58, 2444-2448.
18. De Weger, L.A., Dunbar, P., Mahafee, W.F., Lugtenberg, B.J.J. and Sayler, G.S. (1991) Use of bioluminescence markers to detect Pseudomonas spp. in the rhizosphere. Appl. Environ. Microbiol. 57, 3641-3644.
19. O'Kane, D.J., Lingle, W.L., Wampler, J.E., Legocki, M., Legocki, R.P. and Szalay, A.A. (1988) Visualization of bioluminescence as a marker of gene expression in rhizobium-infected soybean root nodules. Plant Mol. Biol. 10, 387-399.
20. Rattray, E.A.S., Prosser, J.I., Glover, L.A. and Killham, K. (1995) Characterization of rhizosphere colonization by luminescent Enterobacter cloacae at the population and single-cell levels. Appl. Environ. Microbiol. 61, 2950-2957.
21. Shaw, J.J. and Kado, C.I. (1986) Development of a Vibrio bioluminescence gene-set to monitor phytopathogenic bacteria during the ongoing disease process in a non-disruptive manner. Bio/Technology 4, 560-564.
22. Duncan, S., Glover, L.A., Killham, K. and Prosser, J.I. (1994) Luminescence-based detection of activity of starved and viable but nonculturable bacteria. Appl. Environ. Microbiol. 60, 1308-1316.