Wide variation in DNA content among isolates of Trypanosoma brucei ssp.

Acta Tropica

Volumn 63, Issue 2-3, 1997, Pages 75-87

  Kanmogne, Georgette D ^a   Bailey, Mick ^a   Gibson, Wendy C ^a  

^a UNIVERSITY OF BRISTOL   (United Kingdom)

Author keywords

chromosome; DNA content; molecular karyotype; Trypanosoma brucei gambiense

Indexed keywords

ANIMAL EXPERIMENT; ARTICLE; CHROMOSOME; CONTROLLED STUDY; DNA CONTENT; FLOW CYTOMETRY; GENETIC VARIABILITY; KARYOTYPE; NONHUMAN; SOUTHERN BLOTTING; TRYPANOSOMA;

ANIMALS; BLOTTING, SOUTHERN; DNA PROBES; DNA, PROTOZOAN; ELECTROPHORESIS, GEL, PULSED-FIELD; FLOW CYTOMETRY; KARYOTYPING; MICROSATELLITE REPEATS; NUCLEIC ACID HYBRIDIZATION; REPETITIVE SEQUENCES, NUCLEIC ACID; TRYPANOSOMA BRUCEI BRUCEI; TUBULIN;

EID: 0030888619     PISSN: 0001706X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0001-706X(96)00600-6     Document Type: Article

References (31)

1. Bromidge, T., Gibson, W., Hudson, K.M. and Dukes, P. (1993) Identification of Trypanosoma brucei gambiense by PCR amplification of variant surface glycoprotein genes. Acta Trop. 53, 107-119.
2. Cunningham, I. (1977) New culture medium for maintenance of tsetse tissues and growth of trypanosomatids. J. Protozool. 24, 325-329.
3. Dero, B., Zampetti-Bosseler, F., Pays, E. and Steinert, M. (1987) The genome and the antigen gene repertoire of Trypanosoma brucei gambiense are smaller than those of T.b. brucei. Mol. Biochem. Parasitol. 26, 247-256.
4. Dukes, P., Kaukas, A., Hudson, K.M., Asonganyi, T. and Gashumba, J.K. (1989) A new method for isolating Trypanosoma brucei gambiense from sleeping sickness patients. Trans. Roy. Soc. Trop. Med. Hyg. 83, 636-639.
5. Enyaru, J.C.K., Allingham, R., Bromidge, T., Kanmogne, G.D. and Carasco, J.F. (1993) The isolation and genetic heterogeneity of Trypanosoma brucei gambiense from north west Uganda. Acta Trop. 54, 31-39.
6. Feinberg, A.P. and Vogelstein, B. (1983) A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem. 132, 6-13.
7. Gibson, W.C., Marshall, T.F.de C and Godfrey, D.G.(1980) Numerical analysis of enzyme polymorphisms: a new approach to the epidemiology and taxonomy of trypanosomes of the subgenus Trypanozoon. Adv. Parasitol. 18, 175-246.
8. Gibson, W.C. (1986). Will the real Trypanosoma brucei gambiense please stand up. Parasitol. Today 2, 255-257.
9. Gibson, W.C. and Borst, P. (1986). Size-fractionation of the small chromosomes of Trypanozoon and Nannomonas trypanosomes by pulsed field gradient gel electrophoresis. Mol. Biochem. Parasitol. 18, 127-140.
10. Gibson, W.C., Dukes, P. and Gashumba, J.K. (1988) Species-specific DNA probes for the identification of African trypanosomes in tsetse flies. Parasitology 97, 63-73.
11. Gibson, W. and Garside, L. (1991) Genetic exchange in Trypanosoma brucei brucei: variable chromosomal location of housekeeping genes in different trypanosome stocks. Mol. Biochem. Parasitol. 45, 77-90.
12. Gibson, W., Garside, L. and Bailey, M. (1992) Trisomy and chromosome size changes in hybrid trypanosomes from a genetic cross between Trypanosoma brucei rhodesiense and T. b. brucei. Mol. Biochem. Parasitol. 52, 189-200.
13. Godfrey, D.G. and Kilgour, V. (1976) Enzyme electrophoresis in characterizing the causative organism of Gambian trypanosomiasis. Trans. Roy. Soc. Trop. Med. Hyg. 70, 219-224.
14. Godfrey, D.G., Scott, C.M., Gibson, W.C., Mehlitz, D. and Zillmann, U. (1987) Enzyme polymorphism and the identity of Trypanosoma brucei gambiense. Parasitology 94, 337-347.
15. Hide, G., Cattand, P., Le Ray, D., Barry, J.D. and Tait, A. (1990) The identification of Trypanosoma brucei sub-species using repetitive DNA sequences. Mol. Biochem. Parasitol. 39, 213-226.
16. Kanmogne, G.D., Stevens, J.R., Asonganyi, T. and Gibson, W.C. (1996a) Characterization of Trypanosoma brucei gambiense isolates using restriction fragment length polymorphisms in 5 variant surface glycoprotein genes. Acta Trop. 61, 239-254.
17. Kanmogne, G.D., Stevens, J.R., Asonganyi, T. and Gibson, W.C. (1996b) Genetic heterogeneity in Trypanosoma brucei gambiense genome analysed by random amplification of polymorphic DNA. Parasitol. Res. 82, 535-541.
18. Masiga, D.K., Smyth, A.J., Hayes, P., Bromidge, T.J. and Gibson, W.C. (1992) Sensitive detection of trypanosomes in tsetse flies by DNA amplification. Int. J. Parasitol. 22, 909-918.
19. Mathieu-Daudé, F., Stevens, J., Welsh, J., Tibayrenc, M. and McClelland, M. (1995) Genetic diversity and population structure of Trypanosoma brucei: clonality versus sexuality. Mol. Biochem. Parasitol. 72, 89-101.
20. Mehlitz, D., Zillmann, U., Scott, C.M. and Godfrey, D.G. (1982) Epidemiological studies in the animal reservoir of gambiense sleeping sickness. Part III. Characterization of Trypanozoon stocks by isoenzymes and sensitivity to human serum. Tropenmed. Parasitol. 33, 113-118.
21. Morasca, L. and Erba, E. (1986). Flow Cytometry. In Animal Cell Culture, IRL Press, Oxford, pp. 125-148.
22. Mühlpfordt, H. and Berger, J. (1989). Characterization and grouping of Trypanosoma brucei brucei, T. b. gambiense and T. b. rhodesiense by quantitative DNA-cytofluorometry and discriminant analysis. Trop. Med. Parasitol. 40, 1-8.
23. Paindavoine, P., Pays, E., Laurent, M., Geltmeyer, Y., Le Ray, D., Mehlitz, D. and Steinert, M. (1986) The use of DNA hybridization and numerical taxonomy in determining relationships between Trypanosoma brucei stocks and subspecies. Parasitology 92, 31-50.
24. Pays, E., Dekerck.P., Van Assel, S., Babiker, E.A., Le Ray, D., Van Meirvenne, N. and Steinert, M. (1983) Comparative analysis of a Trypanosoma brucei gambiense antigen gene family and its potential use in the epidemiology of sleeping sickness. Mol. Biochem. Parasitol. 7, 63-74.
25. Sloof, P., Bos, J.L., Konings A.F.J.M., Menke, H.H., Borst, P., Gutteridge, W.E. and Leon, W. (1983). Characterization of satellite DNA in Trypanosoma brucei and Trypanosoma cruzi. J. Mol. Biol. 167, 1-21.
26. Southern, E.M. (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98, 503-517.
27. Tait, A., Babiker, E.A. and Le Ray, D. (1984). Enzyme variation in Trypanosoma brucei spp. I.Evidence for the subspeciation of Trypanosoma brucei gambiense. Parasitology 89, 311-326.
28. Truc, P. and Tibayrenc, M. (1993) Population genetics of Trypanosoma brucei in Central Africa: taxonomic and epidemiological significance. Parasitology 106, 1-13.
29. Thomashow, L.S., Milhausen, M., Rutter, W.J. and Agabian, N. (1983) The tubulin genes are tandemly linked and clustered in the genome of Trypanosoma brucei. Cell 32, 35-43.
30. Van der Ploeg, L.H.T., Schwartz, D.C., Cantor, C.R. and Borst, P. (1984a). Antigenic variation in Trypanosoma brucei analysed by electrophoretic separation of chromosome-sized DNA molecules. Cell 37, 77-84.
31. Van der Ploeg, L.H.T., Liu, A.Y.C. and Borst, P. (1984b). Structure of the growing telomeres of trypanosomes. Cell 36, 459-468.