Cultured adult rabbit myocytes: Effect of adding supplements to the medium, and response to isoprenaline

Journal of Cardiovascular Electrophysiology

Volumn 8, Issue 9, 1997, Pages 1020-1030

  Mitcheson, John S ^a   Hancox, Jules C ^a   Levi, Allan J ^a  

^a UNIVERSITY OF BRISTOL   (United Kingdom)

Author keywords

Calcium current; Cardiac myocytes; Culture; Isoprenaline; Rabbit

Indexed keywords

CALCIUM CHANNEL; CALCIUM ION; CARNITINE; CREATININE; ISOPRENALINE; TAURINE;

ANIMAL CELL; ARTICLE; CALCIUM CURRENT; CHANNEL GATING; CONCENTRATION RESPONSE; CONTROLLED STUDY; CULTURE MEDIUM; HEART CELL CULTURE; NONHUMAN; PRIORITY JOURNAL; RABBIT; TIME;

EID: 0030886476     PISSN: 10453873     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1540-8167.1997.tb00626.x     Document Type: Article

References (27)

1. Borg TK, Rubin K, Lundgren E, et al: Recognition of extracellular matrix components by neonatal and adult cardiac myocytes. Dev Biol 1984;104:86-96.
2. Jacobson SL, Piper HM: Cell cultures of adult cardiomyocytes as models of the myocardium. J Mol Cell Cardiol 1986;18:661-678.
3. Mitcheson JS, Hancox J, Levi AJ: Action potentials, ion channel currents and transverse tubule density in adult rabbit ventricular myocytes maintained for 6 days in cell culture. Pflügers Arch 1996;431:814-827.
4. Ellingsen O, Davidoff AJ, Prasad SK, et al: Adult rat ventricular myocytes cultured in defined medium: Phenotype and electromechanical function. Am J Physiol 1993;265(Heart Circ Physiol):H747-H754.
5. Schackow TE, Decker RS, Ten Eick RE: Electrophysiology of adult cat cardiac ventricular myocytes: Changes during primary culture. Am J Physiol 1995;268(Cell Physiol):C1002-C1017.
6. Volz A, Piper HM, Siegmund B, et al: Longevity of adult ventricular rat heart muscle cells in serum-free primary culture. J Mol Cell Cardiol 1991;23:161-173.
7. Berger HJ, Prasad SK, Davidoff AJ, et al: Continual electric field stimulation preserves contractile function of adult ventricular myocytes in primary culture. Am J Physiol 1994;266(Heart Circ Physiol):H341-H349.
8. Prichard BNC, Owens CWI, Smith CCT, et al: Heart and catecholamines. Acta Cardiol 1991;46:309-322.
9. Castle NA, Haylett DG, Jenkinson DH: Toxins in the characterisation of potassium channels. TINS 1989;12:59-65.
10. Hancox J, Levi AJ, Lee CO, et al: A method for isolating rabbit atrioventricular node myocytes which retain normal morphology and function. Am J Physiol 1993;265:H755-H766.
11. Seamon KB, Daly JW: Forskolin: Its biological and chemical properties. Adv Cyclic Nucleotide Protein Phosphoryl Res 1986;20:1-150.
12. Bean BP, Nowycky MC, Tsien RW: β-Adrenergic modulation of calcium channels in frog ventricular heart cells. Nature 1984;307:371-375.
13. Tsien RW: Adrenaline-like effects of intracellular iontophoresis of cyclic AMP in cardiac Purkinje fibres. Nature 1973;245:120-121.
14. Tsien RW, Giles WR, Greengard P: Cyclic AMP mediates the effect of adrenaline on cardiac Purkinje fibres. Nature 1972;240:181-183.
15. Isenberg G, Klockner U: Calcium currents of isolated bovine ventricular myocytes are fast and of large amplitude. Pflügers Arch 1982;395:30-41.
16. Kato S, Ivester CT, Cooper G. et al: Growth effects of electrically stimulated contraction of adult feline cardiocytes in primary culture. Am J Physiol 1995;268:H2495-H2504.
17. McDonald TF, Pelzer S, Trautwein W, et al: Regulation and modulation of calcium channels in cardiac, skeletal and smooth muscle cells. Physiol Rev 1994;74:365-507.
18. Clark WA, Rudnick SJ, Simpson DG, et al: Cultured adult cardiac myocytes maintain protein synthetic capacity of intact adult hearts. Am J Physiol 1993;264(Heart Circ Physiol):H573-H582.
19. Long CS, Kariya K, Karns L, et al: Sympathetic modulation of the cardiac myocyte phenotype: Studies with a cell-culture model of myocardial hypertrophy. Basic Res Cardiol 1992;87:19-31.
20. Schluter KD, Piper HM: Trophic effects of catecholamines and parathyroid hormone on adult ventricular cardiomyocytes. Am J Physiol 1992;263(Heart Circ Physiol):H1739-H1746.
21. Tiaho F, Nargeot J, Richard S: Voltage-dependent regulation of L-type cardiac Ca channels by isoproterenol. Pflügers Arch 1991;419:596-602.
22. Xie LH, Horie M, James AF, et al: Endothelin-1 inhibits L-type Ca currents enhanced by isoproterenol in guinea-pig ventricular myocytes. Pflügers Arch 1996;431:533-539.
23. Kirshenbaum LA, MacLellan WR, Mazur W, et al: Highly efficient gene transfer into adult ventricular myocytes by recombinant adenovirus. J Clin Invest 1993;92:381-387.
24. Schneider MD, MacLellan WR, Kirshenbaum LA, et al: Molecular approaches to cardiac development and hypertrophy. Tex Heart Inst J 1994;21:2-5.
25. Schneider MD, French BA: The advent of adenovirus: Gene therapy for cardiovascular disease. Circulation 1993;88:1937-1942.
26. Bugaisky LB: The use of cell culture to study cardiac myocyte growth and adaptation to stress. J Appl Cardiol 1988;3:311-326.
27. Banach K, Bunemann M, Huser J, et al: Serum contains a potent factor that decreases β-adrenergic receptor-stimulated L-type Ca current in cardiac myocytes. Pflügers Arch 1993;423:245-250.