Site-directed mutagenesis of putative active site residues of MunI restriction endonuclease: Replacement of catalytically essential carboxylate residues triggers DNA binding specificity

Biochemistry

Volumn 36, Issue 37, 1997, Pages 11086-11092

  Lagunavicius, Arunas ^a   Siksnys, Virginijus ^a  

^a VILNIUS UNIVERSITY   (Lithuania)

Author keywords

[No Author keywords available]

Indexed keywords

RESTRICTION ENDONUCLEASE;

AMINO ACID SUBSTITUTION; ARTICLE; BINDING AFFINITY; CARBOXYLATION; CATALYSIS; CRYSTAL STRUCTURE; DNA BINDING; ENZYME ACTIVE SITE; ENZYME SPECIFICITY; NONHUMAN; PRIORITY JOURNAL; RESIDUE ANALYSIS; SITE DIRECTED MUTAGENESIS; X RAY ANALYSIS;

BINDING SITES; CARBOXYLIC ACIDS; CATALYSIS; CHROMOSOME MAPPING; CRYSTALLOGRAPHY, X-RAY; DEOXYRIBONUCLEASE ECORI; DEOXYRIBONUCLEASES, TYPE II SITE-SPECIFIC; DNA; MAGNESIUM; MANGANESE; MUTAGENESIS, SITE-DIRECTED; SEQUENCE ALIGNMENT;

EID: 0030886218     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi963125i     Document Type: Article

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