Analysis of deamidation of small, acid-soluble spore proteins from Bacillus subtilis in vitro and in vivo

Journal of Bacteriology

Volumn 179, Issue 19, 1997, Pages 6020-6027

  Hayes, Christopher S ^a   Setlow, Peter ^a  

^a UNIVERSITY OF CONNECTICUT HEALTH CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ASPARAGINE; ASPARTIC ACID; BACTERIAL DNA; BACTERIAL PROTEIN;

ARTICLE; BACILLUS SUBTILIS; BACTERIAL SPORE; CONTROLLED STUDY; DEAMINATION; ESCHERICHIA COLI; EXTRACTION; NONHUMAN; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN DNA BINDING; PROTEIN PURIFICATION; SITE DIRECTED MUTAGENESIS;

BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; TRIXIS;

EID: 0030884116     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.179.19.6020-6027.1997     Document Type: Article

References (41)

1. Aiyar, A., and J. Leis. 1993. Modification of the megaprimer method of PCR mutagenesis: improved amplification of the final product. BioTechniques 14:366-369.
2. Alarcon-Hernandez, E., and P. Setlow. Unpublished results.
3. Anagnostopoulos, C., and J. Spizizen. 1961. Requirements for transformation in Bacillus subtilis. J. Bacteriol. 81:741-746.
4. Brennan, T. V., J. W. Anderson, Z. Jia, E. B. Waygood, and S. Clarke. 1994. Repair of spontaneously deamidated HPr phosphocarrier protein catalyzed by the L-isoaspartate-(D-aspartate) O-methyltransferase. J. Biol. Chem. 269: 24586-24595.
5. Cabrera-Martinez, R. M., J. M. Mason, B. Setlow, W. M. Waites, and P. Setlow. 1989. Purification and amino acid sequence of two small, acid-soluble proteins from Closiridium bifermentans spores. FEMS Microbiol. Lett. 52: 139-143.
6. Cano, R. J., and M. K. Borucki. 1995. Revival and identification of bacterial spores in 25-to 40-million-year-old Dominican amber. Science 268:1060-1064.
7. Clarke, S. 1987. Propensity for spontaneous succinimide formation trom aspartyl and asparaginyl residues in cellular proteins. Int. J. Pept. Protein Res. 30:808-821.
8. Connors, M. J., and P. Setlow. 1985. Cloning of a small, acid-soluble spore protein gene from Bacillus subtilis and determination of its complete nucleotide sequence. J. Bacteriol. 161:333-339.
9. Francesconi, S. C., T. J. MacAlister, B. Setlow, and P. Setlow. 1988. Immunoelectron microscopic localization of small, acid-soluble spore proteins in sporulating cells of Bacillus subtilis. J. Bacteriol. 170:5963-5967.
10. Galletti, P., D. Ingrosso, C. Manna, G. Clemente, and V. Zappia. 1995. Protein damage and methylation-mediated repair in the erythrocyte. Biochem. J. 306:313-325.
11. Geiger, T., and S. Clarke. 1987. Deamidation, isomerization, and racemization at asparaginyl and aspartyl residues in peptides. Succinimide-linked reactions that contribute to protein degradation. J. Biol. Chem. 262:785-794.
12. Goldrick, S., and P. Setlow. 1983. Expression of a Bacillus megaterium sporulation-specific gene during sporulation of Bacillus subtilis. J. Bacteriol. 155: 1459-1462.
13. Hackett, R. H., and P. Setlow. 1988. Properties of spores of Bacillus subtilis strains which lack the major small, acid-soluble protein. J. Bacteriol. 170: 1403-1404.
14. Hayes, C. S., and P. Setlow. 1997. Unpublished results.
15. Li, C., and S. Clarke. 1992. Distribution of an L-isoaspartyl protein methyltransferase in eubacteria. J. Bacteriol. 174:355-361.
16. Lowry, O. H., N. J. Rosebrough, A. L. Farr, and R. J. Randall. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265-275.
17. Lura, R., and V. Schirch. 1988. Role of peptide conformation in the rate and mechanism of deamidation of asparaginyl residues. Biochemistry 27:7671-7677.
18. Magill, N. G., A. E. Cowan, M. A. Leyva-Vazquez, M. Brown, D. E. Koppel, and V. Setlow. 1996. Analysis of the relationship between the decrease in pH and accumulation of 3-phosphoglyceric acid in developing forespores of Bacillus species. J. Bacteriol. 178:2204-2210.
19. Mason, J. M., and P. Setlow. 1986. Essential role of small, acid-soluble spore proteins in resistance of Bacillus subtilis spores to UV light. J. Bacteriol. 167:174-178.
20. Mohr, S., and P. Setlow. Unpublished results.
21. Nicholson, W, L., and P. Setlow. 1990. Sporulation, germination, and outgrowth, p. 391-450. In C. R. Harwood and S. M. Cutting (ed.), Molecular biological methods for Bacillus. John Wiley & Sons Ltd., Chichester, England.
22. Oliyai, C., J. P. Patel, L. Carr, and R. T. Borchardt. 1994. Solid state chemical instability of an asparaginyl residue in a model hexapeptide. J. Pharm. Sci. Technol 48:167-173.
23. Popham, D. L., S. Sengupta, and P. Setlow. 1995. Heat, hydrogen peroxide, and UV resistance of Bacillus subtilis spores with increased core water content and with or without major DNA-binding proteins. Appl. Environ. Microbiol. 61:3633-3638.
24. Rao, H., S. C. Mohr, H. Fairhead, and P. Setlow. 1992. Synthesis and characterization of a 29-amino acid residue DNA-binding peptide derived from α/β-type small, acid-soluble spore proteins (SASP) of bacteria. FEBS Lett. 305:115-120.
25. Reisfield, R. A., V. J. Lewis, and D. E. Williams. 1962. Disk electrophoresis of basic proteins and peplides on polyacrylamide gels. Nature 195:281-283.
26. Russell, A. D. 1982. The destruction of bacterial spores. Academic Press, New York, N.Y.
27. Setlow, B., A. R. Hand, and P. Setlow. 1991. Synthesis of a Bacillus subtilis small, acid-soluble spore protein in Escherichia coli causes cell DNA to assume some characteristics of spore DNA. J. Bacteriol. 173:1642-1653.
28. Setlow, B., and P. Setlow. 1995. Binding to DNA protects α/β-type, small, acid-soluble spore proteins of Bacillus and Clostridium species against digestion by their specific protease as well as by other proteases. J. Bacteriol. 177:4149-4151.
29. Setlow, B., D. Sun, and P. Setlow. 1992. Interaction between DNA and α/β-type small, acid-soluble spore proteins: a new class of DNA-binding protein. J. Bacteriol. 174:2312-2322.
30. Setlow, P. 1975. Identification and localization of the major proteins degraded during germination of Bacillus megaterium spores. J. Biol. Chem. 250:8159-8167.
31. Setlow, P. 1988. Small, acid-soluble spore proteins of Bacillus species: structure, synthesis, genetics, function and degradation. Annu. Rev. Microbiol. 42:319-338.
32. Setlow, P. 1995. Mechanisms for the prevention of damage to DNA in spores of Bacillus species. Annu. Rev. Microbiol. 49:29-54.
33. Setlow, P. Unpublished results.
34. Sharma, S., P. K. Hammen, J. W. Anderson, A. Leung, F. Georges, W, Hengstenberg, R. E. Klevit, and E. B. Waygood. 1993. Deamidation of HPr, a phosphocarrier protein of the phosphoenolpyruvate:sugar phosphotransferase system, involves asparagine 38 (HPr-1) and asparagine 12 (HPr-2) in isoaspartyl add formation. J. Biol. Chem. 268:17695-17704.
35. Stephenson, R. C., and S. Clarke. 1989. Succinimide formation from aspartyl and asparaginyl peptides as a model for the spontaneous degradation of proteins. J. Biol. Chem. 264:6164-6170.
36. Stragier, P., C. Bonamy, and C. Karmazyn-Campelli. 1988. Processing of a sporulation sigma factor in Bacillus subtilis: how morphological structure could control gene expression. Cell 52:697-704.
37. Studier, F. W., and B. A. Moffatt. 1986. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J. Mol. Biol. 189:113-130.
38. + and other monovalent cations in dormant and germinating spores of Bacillus megaterium. J. Bacteriol. 148:20-29.
39. Tovar-Rojo, F., and P. Setlow. 1991. Effects of mutant small, acid-soluble spore proteins from Bacillus subtilis on DNA in vivo and in vitro. J. Bacteriol. 173:4827-4835.
40. Tyler-Cross, R., and V. Schirch. 1991. Effects of amino acid sequence, buffers, and ionic strength on the rate and mechanism of deamidation of asparagine residues in small peptides. J. Biol. Chem. 266:22549-22556.
41. Wright, H. T. 1991. Nonenzymatic deamidation of asparaginyl and glutaminyl residues in proteins. Crit. Rev. Biochem. Mol. Biol. 26:1-52.