Comparative analysis of radiometric and autoradiographic methods in the investigation of DNA synthetic activity of tumor cells in vitro

Oncology Reports

Volumn 4, Issue 6, 1997, Pages 1301-1304

  Gembitsky, Dmitry S ^a   Antokhin, Alexander I ^b   Popova, Natalia Y ^b   Podusov, Yuri N ^c   Romanov, Yuri A ^b  

^a CREIGHTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b PIROGOV RUSSIAN NATIONAL RESEARCH MEDICAL UNIVERSITY   (Russian Federation)

^c Municipal Hospital No 7 of Sevastopol City   (Ukraine)

Author keywords

Autoradiography; Cell cycle; Cell proliferation inhibitors; DNA synthesis; Ehrlich ascites tumor; Liquid scintillation radiometry

Indexed keywords

ARTICLE; AUTORADIOGRAPHY; CELL CYCLE S PHASE; CELL PROLIFERATION; DNA SYNTHESIS; EHRLICH ASCITES TUMOR; GROWTH REGULATION; HUMAN; HUMAN CELL; LIQUID SCINTILLATION COUNTING; PRIORITY JOURNAL;

EID: 0030883412     PISSN: 1021335X     EISSN: None     Source Type: Journal    
DOI: 10.3892/or.4.6.1301     Document Type: Article

References (14)

1. Gembitsky DS, Antokhin AI, Popova NY and Romanov YA: Investigation of the role of beta-adrenergic receptors of Ehrlich ascites tumor in regulation of the tumor cell proliferation by adrenergic ligands. Bull Exp Biol Med 120: 525-528, 1995.
2. Gembitsky DS, Antokhin AI, Romanov YA and Popova NY: Levels of DNA synthesis in Ehrlich ascites tumor cells in culture, treated with different concentrations of chalone-containing preparation. Bull Exp Biol Med 114: 1883-1885, 1992.
3. Gembitsky DS, Karpets LZ, Adrianov NV, Antokhin AI and Romanov YA: Determination of the biological activity of chalone-containing preparation from Ehrlich ascitic carcinoma and its fractions obtained by high performance liquid chromatography in a cell culture derived from this tumor. Bull Exp Biol Med 117: 83-86, 1994.
4. Eblen ST, Fautsch MP, Burnette RJ, Snyder M and Leof EB: Dissociation of p34cdc2 complex formation from phosphorylation and histone H1 kinase activity. Cancer Res 55: 1994-2000, 1995.
5. Kriek JA, van der Walt BJ and Bester AJ: Isolation and characterization of adult bovine heart muscle chalones. Proc Natl Acad Sci USA 78: 4161-4164, 1991.
6. Nieto-Sampedro M: Astrocyte mitogen inhibitor related to epidermal growth factor receptor. Science 240: 1784-1786, 1988.
7. Romanov YA, Ketlinsky SA, Antokhin AI and Okulov VB: Chalones and regulation of cell division. Medicina Publ, Moscow, 1984.
8. Barbason H, Bouzahzah B, Herens C, Marchandise J, Sulon J and van Cantfort J: Circadian synchronization of liver regeneration in adult rats: the role played by adrenal hormones. Cell Tissue Kinet 22: 451-460, 1989.
9. Paulsen JE, Hall KS, Rugstad HE, Reichelt KL and Elgjo KL: The synthetic hepatic peptides pyroglutamylglutamylglycylscrylasparagine and pyroglutamylglutamylglycylserylaspartic acid inhibit growth of MHIC1 rat hepatoma cells transplanted into Buffalo rats or athymic mice. Cancer Res 52: 1218-1221, 1992.
10. Paus R, Stenn KS and Elgjo K: The epidermal pentapeptide pyroGlu-Glu-Asp-Ser-GlyOH inhibits murine hair growth in vivo and in vitro. Dermatologica 183: 173-178, 1991.
11. Popova NI, Antokhin AI, Adrianov NV, Tret'iakova LZ and Romanov IA: Fractionation of a chalone-containing substance from Ehrlich's ascites tumor using high pressure chromatography. Bull Exp Biol Med 112: 418-419, 1991.
12. Elgjo K and Reichelt KL: Mouse epidermal cell renewal after topical treatment with different concentrations of the epidermal peptide pyroGlu-Glu-Asp-Ser-GlyOH. Virchows Arch (B) 59: 89-93, 1990.
13. Gembitsky DS, Popova NY, Antokhin AI, Podusov YN, Karpets LZ, Adrianov NV and Romanov YA: The Ehrlich ascites carcinoma (EAC) contains 300-500 Da inhibitor(s) suppressing DNA synthesis in cultured EAC cells and EAC cell division in tumor-bearing mice. Oncol Rep 3: 333-338, 1996.
14. Savchenko TV, Zakharof VB, Mamontov SG and Romanov YA: Cell proliferation in Ehrlich's ascites tumor and the survival of tumor-bearing mice following administration of a chalone. Vetstnik Acad Med Nauk SSSR 11: 54-59, 1980.