Rapid detection of viable enteroviruses in water by tissue culture and semi-nested polymerase chain reaction

Water Science and Technology

Volumn 35, Issue 11-12, 1997, Pages 429-432

  Murrin, K ^a   Slade, J ^a  

^a THAMES WATER UTILITIES LTD   (United Kingdom)

Author keywords

Detection; Enterovirus; Rapid tests; RT PCR; Viable; Water

Indexed keywords

ELUTION; ENTEROVIRUSES; RAPID DETECTION; SEMINESTED POLYMERASE CHAIN REACTION;

ADSORPTION; ASSAYS; CHEMICAL REACTIONS; ENZYMES; MICROBIOLOGY; POTABLE WATER; PRECIPITATION (CHEMICAL); PROTEINS; SAMPLING; TISSUE CULTURE; VIRUSES;

WATER TREATMENT;

DRINKING WATER;

ADSORPTION; CONFERENCE PAPER; ENTEROVIRUS; PRECIPITATION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TISSUE CULTURE; VIRUS DETECTION; WATER CONTAMINATION; WATER QUALITY; WATER SAMPLING;

ENTEROVIRUS;

EID: 0030882096     PISSN: 02731223     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S0273-1223(97)00298-9     Document Type: Conference Paper

References (5)

1. Boom, R., Sol, C. J. A., Salimans, M. M. M., Jansen, C. L., Wertheim-van Dillen, P. M. E. and Van der Noorda, J. (1990). Rapid and simple method for purification of nucleic acids. J. Clin. Microbiol., 28, 495-503.
2. Hurley, M. A. and Roscoe, M. E. (1983). Automated statistical analysis of microbial enumeration by dilution series. J. Appl. Bacteriol., 55, 159-164. (Program modified by J. R. Rose, University of South Florida).
3. Nicholson, F., Meetoo, G., Aiyar, S., Banatvala, J. E. and Muir, P. (1994). Detection of enterovirus RNA in clinical samples by nested polymerase chain reaction for rapid diagnosis of enterovirus infection. J. Virol. Meth., 48, 155-166.
4. Slade, J. S. (1978). Enteroviruses in slow sand filtered water. J. Inst. Wat. Eng. Sci., 32, 530-536.
5. Slade, J. S., Chisholm, R. G. and Harris, N. R. (1984) Detection of enteroviruses in water by suspended-cell cultures. In "Microbiological Methods for Environmental Biotechnology", Society for Applied Bacteriology.