A conserved cysteine residue in yeast uroporphyrinogen decarboxylase is not essential for enzymatic activity

Canadian Journal of Microbiology

Volumn 43, Issue 8, 1997, Pages 792-795

  Di Flumeri, Celestino ^a   Acheson, Nicholas H ^a   Keng, Teresa ^a,b  

^a MCGILL UNIVERSITY   (Canada)

^b Apex Bioscience Inc   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CYSTEINE; HEME; UROPORPHYRINOGEN DECARBOXYLASE;

BIOSYNTHESIS; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; MUTANT; NONHUMAN; NOTE; PLASMID; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE;

SACCHAROMYCES CEREVISIAE;

EID: 0030881096     PISSN: 00084166     EISSN: None     Source Type: Journal    
DOI: 10.1139/m97-114     Document Type: Note

References (25)

1. Blattner, F.R., Burland, V., Plunkett, G., III., Sofia, H.J., and Daniels, D.L. 1993. Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes. Nucleic Acids Res. 21: 5408-5417.
2. De Verneuil, H., Grandchamp, B., and Nordmann, Y. 1980. Some kinetic properties of human red cell uroporphyrinogen decarboxylase. Biochim. Biophys. Acta, 611: 174-186.
3. De Verneuil, H., Sassa, S., and Kappas, A. 1983. Purification and properties of uroporphyrinogen decarboxylase from human erythrocytes. J. Biol. Chem. 258: 2454-2460.
4. Di Flumeri, C., Larocque, R., and Keng, T. 1993. Molecular analysis of HEM6 (HEM12) in Saccharomyces cerevisiae, the gene for uroporphyrinogen decarboxylase. Yeast, 9: 613-623.
5. Elder, G., Tovey, J.A., and Sheppard, D. M. 1983. Immunochemical evidence for a single protein with decarboxylase activity in human erythrocytes and liver. Biochem. J. 215: 45-55.
6. Felix, F., and Brouillet, N. 1990. Purification and properties of uroporphyrinogen decarboxylase from Saccharomyces cerevisiae. Eur. J. Biochem. 188: 393-403.
7. Garey, J.R., Labbe-Bois, R., Chelstowska, A., Rytka, J., Harrison, L., Kushner, J., and Labbe, P. 1992. Uroporphyrinogen decarboxylase in Saccharomyces cerevisiae-HEM12 gene sequence and evidence for two conserved glycines essential for enzymatic activity. Eur. J. Biochem. 205: 1011-1016.
8. Hoare, D.S., and Heath, H. 1959. The biosynthesis of porphyrins from porphobilinogen by Rhodopseudomonas spheroides. 2. The partial purification and some properties of porphobilinogen deaminase and uroporphyrinogen decarboxylase. Biochem. J. 73: 679-690.
9. Ito, H., Jukuda, Y., Murata, K., and Kimura, A. 1983. Transformation of intact yeast cells treated with alkali cations. J. Bacteriol. 153: 163-168.
10. Kawanishi, S., Seki, Y., and Sano, S. 1983. Uroporphyrinogen decarboxylase. Purification, properties, and inhibition by polychlorinated biphenyl isomers. J. Biol. Chem. 258: 4285-4292.
11. Koopmann, G.E., and Battle, A.M. Del C. 1987. Biosynthesis of porphyrins in Rhodopseudomonas palustris. VI. The effect of metals, thiols, and other reagents on the activity of uroporphyrinogen decarboxylase. Int. J. Biochem. 19: 373-377.
12. Kurlandzka, A., Zoladek, T., Rytka, J., Labbe-Bois, R., and Urban-Grimal, D. 1988. The effects in vivo of mutationally modified uroporphyrinogen decarboxylase in different hem12 mutants of baker's yeast (Saccharomyces cerevisiae). Biochem. J. 253: 109-116.
13. Labbe-Bois, R., and Labbe, P. 1990. Tetrapyrrole and heme biosynthesis in the yeast Saccharomyces cerevisiae. In Biosynthesis of heme and chlorophylls. Edited by H.A. Dailey. McGraw-Hill Book Co., New York. pp. 235-285.
14. Marczynski, G.T., and Shapiro, L. 1992. Cell-cycle control of a cloned chromosomal origin of replication from Caulobacter crescentus. J. Mol. Biol. 226: 959-977.
15. Mukerji, S.K., and Pimstone, N.R. 1987. Evidence for two uroporphyrinogen decarboxylase isoenzymes in human erythrocytes. Biochem. Biophys. Res. Commun. 146: 1196-1203.
16. Nishimura, K., Nakayashiki, T., and Inokuchi, H. 1993. Cloning and sequencing of the hemE gene encoding uroporphyrinogen III decarboxylase (UPD) from Escherichia coli. Gene, 133: 109-113.
17. Romana, M., LeBoulch, P., and Romeo, P.H. 1987. Rat uroporphyrinogen decarboxylase cDNA: nucleotide sequence and comparison to human uroporphyrinogen decarboxylase. Nucleic Acids Res. 17: 7211.
18. Romeo, P.H., Dubart, A., Grandchamp, B., De Verneuil, H., Rosa, J., Nordmann, Y., and Goossens, M. 1984. Isolation and identification of a cDNA clone coding for rat uroporphyrinogen decarboxylase. Proc. Natl. Acad. Sci. U.S.A. 81: 3346-3350.
19. Romeo, P.H., Raich, N., Dubart, A., Beaupain, D., Pryor, M., Kushner, J., Cohen-Solal, M., and Goossens, M. 1986. Molecular cloning and nucleotide sequence of a complete human uroporphyrinogen decarboxylase. J. Biol. Chem. 261: 9825-9831.
20. Straka, J.G., and Kushner, J.P. 1983. Purifcation and characterization of bovine hepatic uroporphyrinogen decarboxylase. Biochemistry, 22: 4664-4672.
21. Tait, G.H. 1978. The biosynthesis and degradation of heme. In Heme and hemoproteins. Edited by F. De Metteis and W.N. Aldridge. Springer-Verlag, New York. pp. 1-48.
22. Tomio, J.M., Garcia, R.C., San Martin de Viale, L.C., and Grinstein, M. 1970. Porphyrin biosynthesis. VII. Porphyrinogen carboxy-lyase from avian erythrocytes. Purification and properties. Biochim. Biophys. Acta, 198: 353-363.
23. Urban-Grimal, D., and Labbe-Bois, R. 1981. Genetic and biochemical characterization of mutants of Saccharomyces cerevisiae blocked in six different steps of heme biosynthesis. Mol. Gen. Genet. 183: 85-92.
24. Wu, C., Xu, W., Kozak, C.A., and Desnick, R.J. 1996. Mouse uroporphyrinogen decarboxylase: cDNA cloning, expression, and mapping. Mamm. Genome, 7: 349-352.
25. Wyckoff, E.A., Phillips, J.D., Sowa, A.M., Franklin, M.R., and Kushner, J.P. 1996. Mutational analysis of human uroporphyrinogen decarboxylase. Biochim. Biophys. Acta, 1298: 294-304.