Alterations in steroidogenesis in alligators (Alligator mississippiensis) exposed naturally and experimentally to environmental contaminants

Environmental Health Perspectives

Volumn 105, Issue 5, 1997, Pages 528-533

  Crain, D Andrew ^a   Guillette Jr , Louis J ^a   Rooney, Andrew A ^a   Pickford, Daniel B ^a,b  

^a University of Florida ^*  (United States)

^b UNIVERSITY OF MANCHESTER   (United Kingdom)

Author keywords

2,4 D; Alligator; Aromatase; Atrazine; Contaminants; Endocrine disruption; Hormones

Indexed keywords

2,4 DICHLOROPHENOXYACETIC ACID; AROMATASE; ATRAZINE; ENVIRONMENTAL CHEMICAL; ESTRADIOL; HERBICIDE; HORMONE; STEROID; TAMOXIFEN; TESTOSTERONE; VINCLOZOLIN;

ALLIGATOR; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CONTROLLED STUDY; DEVELOPMENT; ENDOCRINE FUNCTION; ENVIRONMENTAL EXPOSURE; ENZYME ACTIVITY; ESTRADIOL BLOOD LEVEL; FEMALE; HISTOPATHOLOGY; HORMONE BLOOD LEVEL; LAKE; MALE; MESONEPHROS; NONHUMAN; PRIORITY JOURNAL; REPRODUCTION; SEX TRANSFORMATION; STEROIDOGENESIS; TESTOSTERONE BLOOD LEVEL; UNITED STATES; WATER POLLUTION;

ALLIGATOR; ALLIGATOR MISSISSIPPIENSIS; ALLIGATORINAE; ANIMALIA;

EID: 0030879591     PISSN: 00916765     EISSN: None     Source Type: Journal    
DOI: 10.1289/ehp.97105528     Document Type: Article

References (39)

1. Crain DA, Guillette LJ Jr. Endocrine-disrupting contaminants and reproduction in vertebrate wildlife. Rev Toxicol (in press).
2. McLachlan JA. Functional toxicology: a new approach to detect biologically active xenobiotics. Environ Health Perspect 101:386-387 (1993).
3. Guillette LJ Jr, Crain DA, Rooney AA, Pickford DB. Organization versus activation: the role of endocrine-disrupting contaminants (EDCs) during embryonic development in wildlife. Environ Health Perspect 103(suppl 7):157-164 (1995).
4. Bull JJ. Sex determination in reptiles. Q Rev Biol 55:3-21 (1980).
5. Wibbels T, Bull JJ, Crews D. Synergism between temperature and estradiol: a common pathway in turtle sex determination. J Exp Zool 260:130-134 (1991).
6. Wibbels T, Gideon P, Bull JJ, Crews D. Estrogen-and temperatute-induced medullary cord regression during gonadal differentiation in a turtle. Differentiation 53:149-154 (1993).
7. Crews D, Bull JJ, Wibbels T. Estrogen and sex reversal in turtles: a dose-dependent phenomenon. Gen Comp Endocrinol 81:357-364 (1991).
8. Wibbels T, Crews D. Specificity of steroid hormone-induced sex determination in a turtle. J Endocrinol 133:121-129 (1992).
9. Wibbels T, Bull JJ, Crews D. Temperature-dependent sex determination: a mechanistic approach. J Exp Zool 270:71-78 (1994).
10. Simpson ER, Mahendroo MS, Means GD, Kilgore MW, Hinshelwood MM, Graham-Lorence S, Amarneh B, Ito Y, Fisher CR, Michael MD. Aromatase cytochrome P450, the enzyme responsible for estrogen biosynthesis. Endocr Rev 15:342-355 (1994).
11. Chardard D, Desvages G, Pieau C, Dournon C. Aromatase activity in larval gonads of Pleurodeles waltl (urodele amphibia) during normal sex differentiation and during sex reversal by thermal treatment effect. Gen Comp Endocrinol 99:100-107 (1995).
12. Desvages G, Pieau C. Aromatase activity in gonads of turtle embryos as a function of the incubation temperature of eggs. J Steroid Biochem Mol Biol 41:851-853 (1992).
13. Smith CA, Elf PK, Lang JW, Joss JMP. Aromatase enzyme activity during gonadal sex differentiation in alligator embryos. Differentiation 58:281-290 (1995).
14. Crews D, Cantu AR, Bergeron JM, Rhen T. The relative effectiveness of androstenedione, testosterone, and estrone, precursors to estradiol, in sex reversal in the red-eared slider (Trachemys scripta), a turtle with temperature-dependent sex determination. Gen Comp Endocrinol 100:119-127 (1995).
15. Wibbels T, Crews D. Putative aromatase inhibitor induces male sex determination in a female unisexual lizard and in a turtle with temperature-dependent sex determination. J Endocrinol 141:295-299 (1994).
16. Schelske C, Brezonik P. Can Lake Apopka be restored? In: Restoration of aquatic ecosystems: science, technology and public policy. Washington, DC.National Academy Press, 1992; 393-398.
17. U.S. EPA. Tower Chemical Company Superfund site biological assessment. Athens, GA:EPA Region IV, Environmental Services Division, Ecological Support Branch, 1994.
18. Heinz GH, Percival HF, Jennings ML. Contaminants in American alligator eggs from Lake Apopka, Lake Griffin, and Lake Okeechobee, Florida. Environ Monit Assess 16:277-285 (1991).
19. Guillette LJ Jr, Gross TS, Masson GR, Matter JM, Percival HF, Woodward AR. Developmental abnormalities of the gonad and abnormal sex hormone concentrations in juvenile alligators from contaminated and control lakes in Florida. Environ Health Perspect 102:680-688 (1994).
20. Lang JW, Andrews HV. Temperature-dependent sex determination in crocodilians. J Exp Zool 270:28-44 (1994).
21. Ferguson MWJ. Reproductive biology and embryology of the crocodilians. In: Biology of the Reptilia (Gans C, Billet F, Maderson P, eds). New York: J. Wiley and Sons, 1985:329-491.
22. Lance VA, Bogart MH. Tamoxifen "sex reverses" alligator embryos at male producing temperatures, but is an antiestrogen in female hatchlings: Experientia 47:263-266 (1991).
23. Gray LEJ, Ostby JM, Marshall R. The Fungicide vinclozolin inhibits morphological sex differentiation in the male rat. Biol Reprod 48(suppl):97 (1993).
24. Smith CA, Joss JMP. Steroidogenic enzyme activity and ovarian differentiation in the salt-water crocodile, Crocodylus porosus. Gen Comp Endocrinol 93:232-245 (1994).
25. Humason GL. Animal tissue techniques. 3rd ed. San Francisco:W.H. Freman and Company, 1972.
26. Forbes TR. Studies on the reproductive system of the alligator. IV. Observations on the development of the gonad, the adrenal cortex, and the Müllerian duct. Contrib Embryol 174:131-163 (1940).
27. Folmar LC, Denslow ND, Rao V, Chow M, Crain DA, Enblom J, Marcino J, Guillette LJ Jr. Vitellogenin induction and reduced serum testosterone concentrations in feral male carp (Cyprinus carpio) captured near a major metropolitan sewage treatment plant. Environ Health Perspect 104:1096-1101 (1996).
28. Guillette LJ Jr, Gross TS, Gross D, Rooney AA, Percival HF. Gonadal steroidogenesis in vitro from juvenile alligators obtained from contaminated and control lakes. Environ Health Perspect 103(suppl 4):31-36 (1995).
29. Guillette LJ Jr, Pickford DB, Crain DA, Rooney AA, Percival HF. Reduction in penis size and plasma testosterone concentrations in juvenile alligators living in a contaminated environment. Gen Comp Endocrinol 101:32-42 (1996).
30. Pickford DB. Endocrine regulation of clitero-penis development in the juvenile American alligator (Alligator mississippiensis) [Masters thesis]. University of Florida, Gainesville, FL, 1995.
31. Gross TS, Crain DA, Bjorndal KA, Bolten AB, Carthy RR. Identification of sex in hatchling loggerhead turtles (Caretta caretta) by analysis of steroid concentrations in chorioallantoic/amniotic fluid. Gen Comp Endocrinol 99:204-210 (1995).
32. Connor K, Howell J, Chen I, Liu H, Berhane K, Sciaretta C, Safe S, Zacharewski T. Failure of chloro-s-triazine-derived compounds to induce estrogen receptor-mediated responses in vivo and in vitro. Fundam Appl Toxicol 30:93-101 (1996).
33. Kniewald J, Peruzovic M, Gojmerac T, Milkovic K, Kniewald Z. Indirect influence of s-triazines on rat gonadotropic mechanism at early postnatal period. J Steroid Biochem 27:1095-1100 (1987).
34. Babic-Gojmerac T, Kniewald Z, Kniewald J. Testosterone metabolism in neuroendocrine organs in male rats under atrazine and dethylatrazine influence. J Steroid Biochem 33:141-146 (1989).
35. Kniewald J, Mildner P, Kniewald Z. Effects of s-triazine herbicides on hormone-receptor complex formation, 5α reductase and 3α-hydroxysteroid dehydrogenase activity at the anterior pituitary level. J Steroid Biochem 11:833-838 (1979).
36. Gutzke WHN, Bull JJ. Steroid hormones reverse sex in turtles. Gen Comp Endocrinol 64:368-372 (1986).
37. Lance VA, Bogart MH. Disruption of ovarian development in alligator embryos treated with an aromatase inhibitor. Gen Comp Endocrinol 86:59-71 (1992).
38. Elbrecht A, Smith RG. Aromatase enzyme activity and sex determination in chickens. Science 255:467-470 (1992).
39. Piferrer F, Zanuy S, Carrillo M, Solar II, Devlin RH, Donaldson EM. Brief treatment with an aromatase inhibitor during sex differentitiation causes chromosomally female salmon to develop as normal, functional males. J Exp Zool 270:255-262 (1994).