Rescue of a mutant G-protein by substrate-assisted catalysis

European Journal of Biochemistry

Volumn 249, Issue 1, 1997, Pages 330-336

  Zor, Tsaffrir ^a   Bar Yaacov, Margalith ^a   Elgavish, Sharona ^a   Shaanan, Boaz ^a   Selinger, Zvi ^a  

^a HEBREW UNIVERSITY OF JERUSALEM   (Israel)

Author keywords

cAMP; GTP hydrolysis; GTP binding protein; Substrate assisted catalysis

Indexed keywords

GUANINE NUCLEOTIDE BINDING PROTEIN; GUANOSINE TRIPHOSPHATE DERIVATIVE;

ARTICLE; CATALYSIS; ENZYME ACTIVITY; ENZYME BINDING; ENZYME STRUCTURE; MUTANT; PRIORITY JOURNAL;

EID: 0030879303     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.1997.00330.x     Document Type: Article

References (37)

1. Freissmuth, M., Casey, P. J. & Gilman, A. G. (1989) G proteins control diverse pathways of transmembrane signaling, FASEB J. 3, 2125-2131.
2. Kaziro, Y., Itoh, H., Kozasa, T., Nakafuku, M. & Satoh, T. (1991) Structure and function of signal-transducing GTP-binding proteins, Annu. Rev. Biochem. 60, 349-400.
3. Bourne, H. R., Sanders, D. A. & McCormick, F. (1990) The GTPase superfamily: a conserved switch for diverse cell functions, Nature 348, 125-132.
4. Neer, E. J. (1995) Heterotrimeric G proteins: organizers of transmembrane signals, Cell 80, 249-257.
5. Cassel, D., Levkovitz, H. & Selinger, Z. (1977) The regulatory GTPase cycle of turkey erythrocyte adenylate cyclase, J. Cyclic Nucleotide Res. 3, 393-406.
6. Cassel, D. & Selinger, Z. (1977) Mechanism of adenylate cyclase activation by cholera toxin: inhibition of GTP hydrolysis at the regulatory site, Proc. Natl Acad. Sci. USA 74, 3307-3311.
7. Van-Dop, C., Tsubokawa, M., Bourne, H. R. & Ramachandran, J. (1984) Amino acid sequence of retinal transducin at the site ADP-ribosylated by cholera toxin, J. Biol. Chem. 259, 696-698.
8. s and stimulate adenylyl cyclase in human pituitary tumors, Nature 340, 692-696.
9. Lyons, J., Landis, C. A., Harsh, G., Vallar, L., Grunewald, K., Feichtinger, H., Duh, Q. Y., Clark, O. H., Kawasaki, E., Bourne, H. R. & McCormick, F. (1990) Two G protein oncogenes in human endocrine tumors, Nature 249, 655-659.
10. sα alter stimulation of adenylyl cyclase, J. Biol. Chem. 264, 15 467-15 474.
11. sα, J. Biol. Chem. 264, 15 475-15 482.
12. H genes mutated at codon 61, Cell 44, 167-176.
13. Gibbs, J. B., Schaber, M. D., Allard, W. J., Sigal, I. S. & Scolnick, E. M. (1988) Purification of ras GTPase activating protein from bovine brain, Proc. Natl Acad. Sci. USA 85, 5026-5030.
14. H-ras: an experimental and theoretical study, Biochemistry 33, 3237-3244.
15. Lambright, D. G., Noel, J. P., Hamm, H. E. & Sigler, P. B. (1994) Structural determinants for activation of the α-subunit of a heterotrimeric G protein, Nature 369, 621-628.
16. iα1 induced by GTP hydrolysis, Science 270, 954-960.
17. 4, Nature 372, 276-279.
18. iα1 and the mechanism of GTP hydrolysis, Science 265, 1405-1412.
19. Sternweis, P. C. & Gilman, A. G. (1982) Aluminum: A requirement for activation of the regulatory component of adenylate cyclase by fluoride, Proc. Natl Acad. Sci. USA 79, 4888-4891.
20. Bigay, J., Deterre, P., Pfister, C. & Chabre, M. (1985) Fluoroaluminates activate transducin-GDP by mimicking the γ-phosphate of GTP in its binding site, FEBS Lett. 191, 181-185.
21. 31P NMR spectroscopy of G protein α subunits, J. Biol. Chem. 266, 3396-3401.
22. Cassel, D. & Selinger, Z. (1978) Mechanism of adenylate cyclase activation through the β-adrenergic receptor: catecholamine-induced displacement of bound GDP by GTP, Proc. Natl Acad. Sci. USA 75, 4155-4159.
23. Schramm, M. & Naim, E. (1970) Adenyl cyclase of rat parotid gland, J. Biol. Chem. 245, 3225-3231.
24. Salomon, Y., Londos, C. & Rodbell, M. (1974) A highly sensitive adenylate cyclase assay, Anal. Biochem. 58, 541-548.
25. Zor, T. & Selinger, Z. (1996) Linearization of the Bradford protein assay increases its sensitivity: theoretical and experimental studies, Anal. Biochem. 236, 302-308.
26. Brünger, A. T. (1992) X-PLOR Manual, version 3.1, Yale University, New Haven CT.
27. Zor, T., Halifa, I., Kleinhaus, S., Chorev, M. & Selinger, Z. (1995) m-Acetylanilido-GTP, a novel photoaffinity label for GTP-binding proteins: synthesis and application, Biochem. J. 306, 253-258.
28. Schlichting, I., Almo, S. C., Rapp, G., Wilson, K., Petratos, K., Lentfer, A., Wittinghofer, A., Kabsch, W., Pai, E. F., Petsko, G. A. & Goody, R. S. (1990) Time-resolved X-ray crystallography study of the conformational change in Ha-Ras p21 protein on GTP hydrolysis, Nature 354, 309-315.
29. Pfeuffer, T. & Helmreich, E. J. M. (1975) Activation of pigeon erythrocyte membrane adenylate cyclase by guanylnucleotide analogues and separation of a nucleotide binding protein, J. Biol. Chem. 250, 867-876.
30. Pfeuffer, T. (1977) GTP-binding proteins in membranes and the control of adenylate cyclase activity, J. Biol. Chem. 252, 7224-7234.
31. α-catalyzed GTP hydrolysis, Structure 4, 1277-1290.
32. Carter, P. & Wells, J. A. (1987) Engineering enzyme specificity by 'substrate-assisted catalysis', Science 237, 394-399.
33. Carter, P., Abrahmsen, L. & Wells, J. A. (1991) Probing the mechanism and improving the rate of substrate-assisted catalysis in subtilisin BPN', Biochemistry 30, 6142-6148.
34. Shinde, U. & Inouye, M. (1995) Folding mediated by an intramolecular chaperone: autoprocessing pathway of the precursor resolved via a substrate assisted catalysis mechanism, J. Mol. Biol. 247, 390-395.
35. Corey, D. R., Willett, W. S., Coombs, G. S. & Craik, C. S. (1995) Trypsin specificity increased through substrate-assisted catalysis, Biochemistry 34, 11 521-11 527.
36. Kim, J., Ruzicka, F. & Frey, P. A. (1990) Remodeling hexose-1-phosphate uridylyltransferase: mechanism-inspired mutation into a new enzyme, UDP-hexose synthase, Biochemistry 20, 10 590-10 593.
37. ras and other GTP-binding proteins, Nat. Struct. Biol. 2, 36-44.