A novel 5-iodonucleoside allows efficient nonenzymatic ligation of single-stranded and duplex DNAs

Tetrahedron Letters

Volumn 38, Issue 32, 1997, Pages 5595-5598

  Xu, Yanzheng ^a   Kool, Eric T ^a  

^a UNIVERSITY OF ROCHESTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

OLIGONUCLEOTIDE;

ARTICLE; DNA DETERMINATION; DNA SYNTHESIS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; IN VITRO STUDY; METHODOLOGY; REACTION ANALYSIS;

EID: 0030878982     PISSN: 00404039     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0040-4039(97)01266-5     Document Type: Article

References (22)

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15. It was found (ref. 4) that the 5'-tosyl-oligonucleotides reacted to a significant degree in ammonia, which lowers overall yields and necessitates the use of labile protecting groups, short deprotection times, and HPLC purification to remove degraded products.
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17. 3): 7.49(1H,d,J=8.0), 6.33(1H,t,J=7.0), 4.50-4.38(1H,m), 3.92-3.87(2H,m), 3.85-3.75(1H,m), 3.76-3.58(2H,m), 3.56-3.41(2H,m), 2.71-2.65(2H,m), 2.57-2.41(1H,m), 2.34-2.22(1H,m), 1.96(3H,s), 1.21(12H,d,J=7).
18. 3CN in 100 mM aqueous triethylammonium acetate buffer (pH 7.0).
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20. Deprotection for 24 h (rt) gives similar results and is sufficient for removal of standard protecting groups: G. M. Blackburn, M.J. Gait, Nucleic Acids in Chemistry and Biology, IRL Press, Oxford, 1990, p. 119.
21. The one-pot two-step reaction was carried out using only the top splint (Fig. 2) as described in the text for the first step; after 18 hr (rt) the reaction was diluted with buffer to lower the strand concentration to 1.3μM, and the second splint was added. After another 18 hr the reaction was worked up and the products isolated.
22. The cyclic product was distinguished from undesired dimer by treatment with S1 nuclease (S. Wang, E. T. Kool, Nucleic Acids Res. 1995, 23, 1157.)