SYBR Green I staining of pulsed field agarose gels is a sensitive and inexpensive way of quantitating DNA double-strand breaks in mammalian cells

Nucleic Acids Research

Volumn 25, Issue 14, 1997, Pages 2945-2946

  Kiltie, Anne E ^a   Ryan, Anderson J ^a,b  

^a UNIVERSITY OF MANCHESTER   (United Kingdom)

^b UNIVERSITY OF MANCHESTER   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

DOUBLE STRANDED DNA; THYMIDINE; TRITIUM;

ANIMAL CELL; ARTICLE; BIOPSY; CAMERA; CELL CULTURE; COMPUTER PROGRAM; COST; DNA STRAND BREAKAGE; IMAGE ANALYSIS; MAMMAL CELL; NONHUMAN; PRIORITY JOURNAL; PULSED FIELD GEL ELECTROPHORESIS; QUANTITATIVE ASSAY; RADIOACTIVITY; STAINING;

ANIMALS; BACTERIOPHAGE LAMBDA; DNA; DNA DAMAGE; DNA, VIRAL; ELECTROPHORESIS, GEL, PULSED-FIELD; FLUORESCENT DYES; MAMMALS; ORGANIC CHEMICALS; SENSITIVITY AND SPECIFICITY; SEPHAROSE; STAINING AND LABELING;

ANIMALIA; MAMMALIA; TRITIUM;

EID: 0030877998     PISSN: 03051048     EISSN: None     Source Type: Journal    
DOI: 10.1093/nar/25.14.2945     Document Type: Article

References (4)

1. Blocher,D., Einspenner,M. and Zajackowski,J. (1989) Int. J. Radiat. Biol. 56, 437-448.
2. Ager,D.D., Dewey,W.C., Gardiner,K., Harvey,W., Johnson,R.T. and Waldren,C.A. (1991) Radiat. Res. 122, 181-187.
3. Rosemann,M., Kanon,B., Konings,A.W.T. and Kampinga,H.H. (1993) Int. J. Radiat. Biol. 64, 245-249.
4. Kiltie,A.E., Orton.C.J., Ryan,A., Roberts,S.A., Marples,B., Davidson,S.E., Hunter,R.D., Margison,G.P., West,C.M.L. and Hendry,J.H. (1997) Int. J. Radiat. Biol. Oncol. Phys. (submitted).