Identification of gene loci controlling pectate lyase production and soft-rot pathogenicity in Pseudomonas marginalis

Canadian Journal of Microbiology

Volumn 43, Issue 5, 1997, Pages 425-431

  Liao, Ching Hsing ^a   McCallus, Daniel E ^a   Fett, William F ^a   Kang, Yue Gyu ^b  

^a EASTERN REGIONAL RESEARCH CENTER   (United States)

^b Korea Ginseng Corporation   (South Korea)

Author keywords

Levan; Pectate lyase; Protease; Pyoverdin; Two component regulators

Indexed keywords

LEVAN; PECTATE LYASE; PROTEINASE; SIDEROPHORE; VIRULENCE FACTOR;

ARTICLE; BACTERIAL VIRULENCE; BACTERIUM COLONY; GENE CONTROL; GENE ISOLATION; GENE LOCUS; GENETIC ANALYSIS; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; PSEUDOMONAS; STRUCTURAL GENE;

BACTERIA (MICROORGANISMS); LEMA; NEGIBACTERIA; PSEUDOMONAS; PSEUDOMONAS MARGINALIS; PSEUDOMONAS VIRIDIFLAVA;

EID: 0030877881     PISSN: 00084166     EISSN: None     Source Type: Journal    
DOI: 10.1139/m97-060     Document Type: Article

References (32)

1. Castilho, B.A., Olfson, P., and Casadaban, M.J. 1984. Plasmid insertion mutagenesis and lac gene fusion with Mini-Mu bacteriophage transposon. J. Bacteriol. 158: 488-495.
2. Collmer, A., and Keen, N.T. 1986. The role of pectic enzymes in plant pathogenesis. Annu. Rev. Phytopathol. 24: 383-409.
3. Corbell, N., and Loper, J.E. 1995. A global regulator of secondary metabolite production in Pseudomonas fluorescens Pf-5. J. Bacteriol. 177: 6230-6236.
4. Dische, Z. 1962. Color reaction of ketoses with carbazole and sulfuric acid. In Methods in carbohydrate chemistry. Vol. I. Edited by R.L. Whistler and M.L. Wolfrom. Academic Press, New York. pp. 481-482.
5. Elumalai, R.P., and Mahadevan, A. 1995. Characterization of pectate lyase produced by Pseudomonas marginalis and cloning of pectate lyase genes. Physiol. Mol. Plant Pathol. 46: 109-119.
6. Fett, W.F., and Dunn, M.F. 1989. Exopolysaccharides produced phytopathogenic Pseudomonas syringae pathovars in infected leaves of susceptible hosts. Plant Physiol. 89: 5-9.
7. Gaffney, T.D., Lam, S.T., Ligon, J., Gates, K., Frazelle, A., DiMaio, J., Hill, S., Goodwin, S., Torkewitz, N., Allshouse, A.M., Kempf, H.-J., and Becker, J.O. 1994. Global regulation of expression of antifungal factors by Pseudomonas fluorescens biological control strain. Mol. Plant-Microbe Interact. 7: 455-463.
8. Hofte, M., Buysens, S., Koedam, N., and Cornelis, P. 1993. Zinc affects siderophore-mediated high affinity iron uptake systems in the rhizosphere Pseudomonas-aeruginosa 7NSK2. Biometals, 6: 85-91.
9. Howe, T.R., and Iglewski, B.H. 1984. Isolation and characterization of alkaline protease-deficient mutants of Pseudomonas aeruginosa in vitro and in a mouse eye model. Infect. Immun. 43: 1058-1063.
10. Hrabak, E.M., and Willis, D.K. 1992. The lemA gene required for pathogenicity of Pseudomonas syringae pv. syringae on bean is a member of a family of two-component regulators. J. Bacteriol. 174: 3011-3020.
11. Keen, N.T., Tamaki, S., Kobayashi, D., and Trollinger, D. 1988. Improved broad-host-range plasmids for DNA cloning in Gram-negative bacteria. Gene, 70: 191-197.
12. Laville, J., Voisard, C., Keel, C., Maurhofer, M., Defago, G., and Haas, D. 1992. Global control in Pseudomonas fluorescens mediating antibiotic synthesis and suppression of black root rot of tobacco. Proc. Natl. Acad. Sci. U.S.A., 89: 1562-1566.
13. Lelliott, R.A., Billing, E., and Hayward, A.C. 1966. A determinative scheme for the fluorescent plant pathogenic pseudomonads. J. Appl. Bacteriol. 29: 470-489.
14. Liao, C.-H. 1989. Analysis of pectate lyases produced by soft rot bacteria associated with spoilage of vegetables. Appl. Environ. Microbiol. 55: 1677-1683.
15. Liao, C.-H. 1991. Cloning of pectate lyase gene pel from Pseudomonas fluorescens and detection of sequences homologous to pel in Pseudomonas viridiflava and Pseudomonas putida. J. Bacteriol. 173: 4386-4393.
16. Laio, C.-H., and Wells, J.M. 1987. Diversity of pectolytic fluorescent pseudomonads causing soft rots of fresh vegetables at produce markets. Phytopathology, 77: 673-677.
17. Liao, C.-H., Hung, H.Y., and Chatterjee, A.K. 1988. An extracellular pectate lyase is the pathogenicity factor of the soft-rotting bacterium Pseudomonas viridiflava. Mol. Plant-Microbe Interact. 1: 199-206.
18. Laio, C.-H., McCallus, D.E., and Wells, J.M. 1993. Calcium-dependent pectate lyase production in the soft-rotting bacterium Pseudomonas fluorescens. Phytopathology, 83: 813-818.
19. Liao, C.-H., McCallus, D.E., and Fett, W.F. 1994. Molecular characterization of two gene loci required for production of the key pathogenicity factor pectate lyase in Pseudomonas viridiflava. Mol. Plant-Microbe Interact. 7: 391-400.
20. Liao, C.-H., McCallus, D.E., Wells, J.M., Tzean, S.-S., and Kang, Y.G. 1996. The repB gene required for production of extracellular enzymes in Pseudomonas viridiflava is an analog of the gacA gene of Pseudomonas syringae. Can. J. Microbiol. 42: 177-182.
21. Lund, B.M. 1983. Bacterial spoilage. In Post-harvest pathology of fruits and vegetables. Edited by C. Dennis. Academic Press, London, pp. 219-257.
22. Miller, J.H. 1992. A short course in bacterial genetics: a laboratory manual and handbook for Escherichia coli and related bacteria. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
23. Miller, J.F., Mekalanos, J.J., and Falkow, S. 1989. Coordinate regulation and sensory transduction in the control of bacterial virulence. Science (Washington, D.C.), 243: 916-922.
24. Nasuno, S., and Starr, M.P. 1966. Pectic enzymes of Pseudomonas marginalis. Phytopathology, 56: 1414-1415.
25. Rich, J.J., Kinscherf, T.G., Kitten, T., and Willis, D.K. 1994. Genetic evidence that the gacA gene encodes the cognate response regulator for the lemA sensor in Pseudomonas syringae. J. Bacteriol. 176: 7468-7475.
26. Ruvkun, G.B., and Ausubel, F.M. 1981. A general method for site-directed mutagenesis in prokaryotes. Nature (London), 289: 85-88.
27. Sambrook, J., Fritsch, E.F., and Maniatis, T. 1989. Molecular cloning: a laboratory manual. 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
28. Schwyn, B., and Neilands, J.B. 1987. Universal chemical assay for the detection and determination of siderophores. Anal. Biochem. 160: 47-56.
29. Simon, R., Preifer, U., and Puhler, C. 1983. A broad host range mobilization system for in vitro genetic engineering: transposon mutagenesis. Biotechnology, 1: 784-791.
30. Staskawicz, B., Dahlbeck, D., Keen, N., and Napoli, C. 1987. Molecular characterization of cloned avirulence genes from race 0 and race 1 of Pseudomonas syringae pv. glycinea. J. Bacteriol. 169: 5789-5794.
31. Zucker, M., and Hankin, L. 1970. Regulation of pectate lyase synthesis in Pseudomonas fluorescens and Erwinia carotovora. J. Bacteriol. 104: 13-18.
32. Zucker, M., Hankin, L., and Sands, D. 1972. Factors governing pectate lyase synthesis in soft rot and non-soft rot bacteria. Physiol. Mol. Plant Pathol. 2: 59-67.