Localization of germination-specific spore-lytic enzymes in Clostridium perfringens S40 spores detected by immunoelectron microscopy

FEMS Microbiology Letters

Volumn 152, Issue 2, 1997, Pages 243-247

  Miyata, Shigeru ^a   Kozuka, Satoshi ^b   Yasuda, Yoko ^b   Chen, Yinghua ^a   Moriyama, Ryuichi ^a   Tochikubo, Kunio ^b   Makino, Shio ^a  

^a NAGOYA UNIVERSITY   (Japan)

^b NAGOYA CITY UNIV MEDICAL SCHOOL   (Japan)

Author keywords

Clostridium perfringens spore; Immunoelectron microscopy; Localization; Spore lytic enzyme

Indexed keywords

AMIDASE; IMMUNOGLOBULIN G; LYSOZYME; PEPTIDOGLYCAN;

ARTICLE; BACILLUS SUBTILIS; CLOSTRIDIUM PERFRINGENS; ENZYME ACTIVITY; ENZYME SPECIFICITY; GERMINATION; IMMUNOELECTRON MICROSCOPY; PRIORITY JOURNAL; PROTEIN DEGRADATION;

ANTIBODIES, FUNGAL; CLOSTRIDIUM PERFRINGENS; GLYCOSIDE HYDROLASES; IMMUNOHISTOCHEMISTRY; MICROSCOPY, IMMUNOELECTRON; N-ACETYLMURAMOYL-L-ALANINE AMIDASE; SPORES, FUNGAL;

BACILLUS SUBTILIS; CLOSTRIDIUM PERFRINGENS;

EID: 0030875212     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(97)00204-8     Document Type: Article

References (16)

1. Foster, S.J. and Johnstone, K. (1990) Pulling the triggers: the mechanism of bacterial spore germination. Mol. Microbiol. 4, 137-141.
2. Moir, A. and Smith, D.A. (1990) The genetics of bacterial spore germination. Annu. Rev. Microbiol. 44, 531-553.
3. Moriyama, R., Hattori, A., Miyata, S., Kudoh, S. and Makino, S. (1996) A gene (sleB) encoding a spore cortex-lytic enzyme from Bacillus subtilis and response of the enzyme to L-alanine-mediated germination. J. Bacteriol. 178, 6059-6063.
4. Miyata, S., Moriyama, R., Sugimoto, K. and Makino, S. (1995) Purification and partial characterization of a spore cortex-lytic enzyme of Clostridium perfringens S40 spores. Biosci. Biotech. Biochem. 59, 514-515.
5. Miyata, S., Chen, Y., Moriyama, R. and Makino, S. (1996) Germination-specific spore cortex-lytic enzymes of Clostridium perfringens S40. Twelfth International Spores Conference. University of Winconsin, Madison, WI.
6. Atrih, A., Zöllner, P., Allmaier, G. and Foster, S.J. (1996) Structural analysis of Bacillus subtilis 168 endospore peptidoglycan and its role during differentiation. J. Bacteriol. 178, 6173-6183.
7. Miyata, S., Moriyama, R., Miyahara, N. and Makino, S. (1995) A gene (sleC) encoding a spore cortex-lytic enzyme from Clostridium perfringens S40 spores; cloning, sequence analysis and molecular characterization. Microbiology 141, 2643-2650.
8. Laemmli, U.K. (1979) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
9. Sakae, Y., Yasuda, Y. and Tochikubo, K. (1995) Immunoelectron microscopic localization of one of the spore germination proteins, GerAB, in Bacillus subtilis spores. J. Bacteriol. 177, 6294-6296.
10. Foster, S.J. and Johnstone, K. (1986) The use of inhibitors to identify early events during Bacillus megaterium KM spore germination. Biochem. J. 237, 865-870.
11. Zuberi, A.R., Feavers, I.M. and Moir, A. (1985) Identification of three complementation units in the gerA spore germination locus of Bacillus subtilis. J. Bacteriol. 162, 756-762.
12. Zuberi, A.R., Moir, A. and Feavers, I.M. (1987) The nucleotide sequence and gene organization of the gerA spore germination operon of Bacillus subtilis 168. Gene 51, 1-11.
13. Yasuda, Y., Sakae, Y. and Tochikubo, K. (1996) Immunological detection of the GerA spore germination proteins in the spore integuments of Bacillus subtilis using scanning electron microscopy. FEMS Microbiol. Lett. 139, 235-238.
14. Gerhardt, P. and Murrell, W.G. (1978) Basis and mechanism of spore resistance: a brief preview. In: Spores VII (Chambliss, G. and Vary, J.C., Eds.) pp. 18-19. American Society for Microbiology, Washington, DC.
15. Gould, G.W. (1984) Mechanism of resistance and dormancy. In: The Bacterial Spore (Hurst, A. and Gould, G.W., Eds.), Vol. 2, pp. 173-209. Academic Press, London.
16. Crafts-Lighty, A. and Ellar, D.J. (1980) The structure and function of the spore outer membrane in dormant and germinating spores of Bacillus megaterium. J. Appl. Bacteriol. 48, 135-145.