Urea-induced conformational changes in cold- and heat-denatured states of a protein, Streptomyces subtilisin inhibitor

Protein Science

Volumn 6, Issue 10, 1997, Pages 2242-2249

  Konno, Takashi ^a,c   Kamatari, Yuji O ^a   Kataoka, Mikio ^b   Akasaka, Kazuyuki ^a  

^a KOBE UNIVERSITY   (Japan)

^b OSAKA UNIVERSITY   (Japan)

^c NATIONAL INSTITUTE FOR PHYSIOLOGICAL SCIENCES   (Japan)

Author keywords

Circular dichroism; Nuclear magnetic resonance; Small angle X ray scattering; Streptomyces subtilisin inhibitor; Urea denatured state

Indexed keywords

SUBTILISIN; UREA;

ARTICLE; COLD; HEAT; NONHUMAN; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN DENATURATION; PROTEIN STABILITY; STREPTOMYCES;

EID: 0030874963     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1002/pro.5560061019     Document Type: Article

References (27)

1. Damaschun G, Damaschun H, Gast K, Gernat C, Zirwer D. 1991. Acid denatured apo-cytochrome c is a random coil: Evidence from small-angle X-ray scattering and dynamic light scattering. Biochim Biophys Acta 1078:289-295.
2. Damaschun G, Damaschun H, Gast K, Misselwitz R, Müller JJ, Pfeil W, Zirwer D. 1993. Cold denaturation-induced conformational changes in phosphoglycerate kinase from yeast. Biochemistry 32:7739-7746.
3. Dill KA, Shortle D. 1991. Denatured states of proteins. Annu Rev Biochem 60:795-825.
4. Dobson CM. 1992. Unfolded proteins, compact states and molten globules. Curr Opin Struct Biol 2:6-12.
5. Flanagan JM, Kataoka M, Fujisawa T, Engelman DM. 1993. Mutation can cause large changes in the conformation of a denatured protein. Biochemistry 32:10359-10370.
6. Glatter O, Kratky O. 1982. Small angle X-ray scattering. New York: Academic Press.
7. Goto Y, Takahashi N, Fink AL. 1990. Mechanism of acid-induced folding of proteins. Biochemistry 29:3480-3488.
8. Guinier A, Fournet B. 1955. Small-angle scattering of X-rays. New York: John Wiely.
9. Hiromi K, Akasaka K, Mitsui Y, Tonomura B, Murao S. 1985. Protein protease inhibitor - The case of Streptomyces subtilisin inhibitor (SSI). Amsterdam: Elsevier.
10. Inouye K, Tonomura B, Hiromi K, Sato S, Murao S. 1977. The states of tyrosyl and tryptophyl residues in a protein proteinase inhibitor (Streptomyces Subtilisin inhibitor). J Biochem 82:1207-1215.
11. Kamatari, YO., Konno, T. Kataoka M, Akasaka K. 1996. Methanol-induced compact and expanded denatured conformations of cytochrome c. J Mol Biol 259:512-523.
12. Kataoka M, Head JF, Persechini A, Kretsinger RH, Engelman DM. 1991. Small-angle X-ray scattering studies of calmodulin mutants with depletions in the linker region of the central helix indicate that the linker region retains a predominantly a-helical conformation. Biochemistry 30:1188-1192.
13. Kataoka M, Hagihara Y, Mihara K, Goto Y. 1993. Molten globule of cytochrome c studied by small angle x-ray scattering. J Mol Biol 229:591-596.
14. Kataoka M, Nishii I, Fujisawa T, Ueki T, Tokunaga F, Goto Y. 1995. Structural characterization of the molten globule and native states of apomyoglobin by solution x-ray scattering. J Mol Biol 249:215-228.
15. Konno T, Kataoka M, Kamatari Y, Kanaori K, Nosaka A, Akasaka K. 1995. Solution x-ray scattering analysis of cold-, heat-, and urea-denatured states of a protein, Streptomyces subtilisin inhibitor. J Mol Biol 251:95-103.
16. Konno T, Morishima I. 1993. Transition state of an unfolding step in human cyanomet myoglobin. Biochim Biophys Acta 1162:93-98.
17. Kuwajima K. 1989. The molten globule state as a clue for understanding the folding and cooperativity of globular-protein structure. Proteins Struct Funct Genet 6:87-103.
18. Mitsui Y, Satow Y, Watanabe Y, Iitaka Y. 1979. Crystal structure of a bacterial protein proteinase inhibitor (Streptomyces Subtilisin inhibitor) at 2.6 ± resolution. J Mol Biol 131:697-724.
19. Ohgushi M, Wada A. 1983. 'Molten-globule state': A compact form of globular proteins with mobile side-chains. FEBS Lett 164:21-24.
20. Pace CN. 1986. Methods Enzymol 131:266-280.
21. Ptitsyn OB. 1992. The molten globule state. In: Creighton EE, ed. Protein folding. New York: W. H. Freeman and Company. pp 243-300.
22. Sato S, Murao S. 1973. Isolation and crystallization of microbial alkaline protease inhibitor, S-SI. Agric Biol Chem 37:1067-1074.
23. Schellman JA. 1987. Selective binding and solvent denaturation. Biopolymers 26:549-559.
24. Shortle D. 1993. Denatured states of proteins and their roles in folding and stability. Curr Opin Struct Biol 3:66-74.
25. Tamura A, Kimura K, Akasaka K. 1991a. Cold denaturation and heat denaturation of Streptomyces subtilisin inhibitor. 1. CD and DSC studies. Biochemistry 30:11313-11320.
26. 1H NMR studies. Biochemistry 30:11313-11320.
27. Ueki T, Hiragi Y, Kataoka M, Inoko Y, Amemiya Y, Izumi Y, Tagawa H, Muroga Y. 1985. Aggregation of bovine serum albumin upon cleavage of its disulfide bonds, studied by the time-resolved small-angle x-ray scattering technique with synchrotron radiation. Biophys Chem 23:115-124.