The human mitochondrial elongation factor tu (EF-Tu) gene: CDNA sequence, genomic localization, genomic structure, and identification of a pseudogene

Gene

Volumn 197, Issue 1-2, 1997, Pages 325-336

  Ling, Mingfu ^a,b   Merante, Frank ^a,b   Chen, Hai Shiene ^a,b   Duff, Catherine ^a,b   Duncan, Alessandra M V ^c   Robinson, Brian H ^a,b  

^a HOSPITAL FOR SICK CHILDREN   (Canada)

^b UNIVERSITY OF TORONTO   (Canada)

^c QUEEN S UNIVERSITY   (Canada)

Author keywords

Northern blot; Nuclear encoded; Pseudogene

Indexed keywords

COMPLEMENTARY DNA; ELONGATION FACTOR 1ALPHA; ELONGATION FACTOR TU; MESSENGER RNA; SIGNAL PEPTIDE;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIUM; BOVIDS; CHROMOSOME 16P; CHROMOSOME 17Q; CYTOSOL; GENE MAPPING; GENE STRUCTURE; HUMAN; HUMAN CELL; LIVER; MITOCHONDRION; MOLECULAR CLONING; NONHUMAN; NORTHERN BLOTTING; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN TARGETING; PSEUDOGENE; SEQUENCE HOMOLOGY; YEAST;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CELL LINE; CHROMOSOME MAPPING; CHROMOSOMES, HUMAN, PAIR 16; CHROMOSOMES, HUMAN, PAIR 17; CLONING, MOLECULAR; CRICETINAE; DNA, COMPLEMENTARY; GENES; HUMANS; HYBRID CELLS; INTRONS; LIVER; MOLECULAR SEQUENCE DATA; PEPTIDE ELONGATION FACTOR TU; PSEUDOGENES; RNA, MESSENGER; SEQUENCE ANALYSIS, DNA; SEQUENCE HOMOLOGY, AMINO ACID; SEQUENCE HOMOLOGY, NUCLEIC ACID;

BACTERIA (MICROORGANISMS); BOVIDAE;

EID: 0030874533     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(97)00279-5     Document Type: Article

References (38)

1. Altschul, S.F., Gish, W., Miller, W., Myers, E.W., Lipman, D.J., 1990. Basic local alignment search tool. J. Mol. Biol. 215, 403-410.
2. An, G., Friesen, J.D., 1980. Nucleotide sequence of tufB and four nearby tRNA structural genes of Escherichia coli. Gene 12, 33-39.
3. Anborgh, P.H., Cool, R.H., Gumusel, F., Harmark, K., Jacquet, E., Weijland, A., Mistou, M.Y., Parmeggiani, A., 1991. Structure-function relationships of elongation factor Tu as studied by mutagenesis. Biochimie 73, 1051-1059.
4. Andersen, C., Wiborg, O., 1994. Escherichia coli elongation factor Tu mutants with decreased affinity for amino acyl-tRNA. Eur. J. Biochem. 220, 739-744.
5. Chomczynski, P., Sacchi, N., 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162, 156-159.
6. Eberly, S.L., Locklear, V., Spremulli, L.L., 1985. Bovine mitochondrial ribosomes elongation factor specificity. J. Biol. Chem. 260, 8721-8725.
7. Leu(UUR) gene associated with the MELAS subgroup of mitochondrial encephalomyopathies. Nature 348, 651-653.
8. Harper, M.E., Saunders, G.F., 1981. Localization of single copy DNA sequence of G-banded human chromosomes by in situ hybridization. Chromosoma 83, 431-439.
9. Hartl, F.-U., Pfanner, N., Nicholson, D.W., Neupert, W., 1989. Mitochondrial protein import. Biochim. Biophys. Acta 988, 1-45.
10. Jonák, J., Anborgh, P.H., Parmeggiani, A., 1994. Histidine-118 of elongation factor Tu: its role in aminoacyl-tRNA binding and regulation of the GTPase activity. FEBS Lett. 343, 94-98.
11. Kawashima, T., Berthet-Colominas, C., Wulff, M., Cusack, S., Leberman, R., 1996. The structure of the Escherichia coli EF-Tu·EF-Ts complex at 2.5 Å resolution. Nature 379, 511-518.
12. Kozak, M., 1991. Structural features in eukaryotic mRNAs that modulate the initiation of translation. J. Biol. Chem. 266, 19867-19870.
13. Lambright, D.G., Noel, J.P., Hamm, H.E., Silger, P.B., 1994. Structural determinants for activation of the a-subunit of a heterotrimeric G protein. Nature 369, 621-628.
14. Lipman, D.J., Pearson, W.R., 1985. Rapid and sensitive protein similarity searches. Science 227, 1435-1440.
15. Ludwig, W., Weizenegger, W.L.M., Betzl, D., Liedel, E., Lenz, T., Ludvigsen, A., Mollenhoff, D., Wenzig, P., Schleifer, K.H., 1990. Complete nucleotide sequences of seven eubacterial genes coding for the elongation factor Tu: functional, structural and phylogenetic evaluations. Arch. Microbiol. 153, 241-247.
16. Merante, F., Petrova-Benedict, R., MacKay, N., Mitchell, G., Lambert, M., Morin, C., De Braekeleer, M., Laframboise, R., Gagné, R., Robinson, B.H., 1993. A biochemically distinct form of cytochrome oxidase (COX) deficiency in the Saguenay-Lac-Saint-Jean region of Quebec. Am. J. Hum. Genet. 53, 481-487.
17. glycine gene. Am. J. Hum. Genet. 55, 437-446.
18. Mesters, J.R., Zeef, L.A.H., Hilgenfeld, R., deGraaf, J.M., Kraal, B., Bosch, L., 1994. The structural and functional basis for the kirromycin resistance of mutant EF-Tu species in Escherichia coli. EMBO J. 13, 4877-4885.
19. Mount, S.M., 1982. A catalogue of splice junction sequences. Nucl. Acids Res. 10, 459-472.
20. Nagata, S., Tsunetsuga-Yokota, Y., Naito, A., Kaziro, Y., 1983. Molecular cloning and sequence determination of the nuclear gene coding for mitochondrial elongation factor Tu of Saccharomyces cerevisiae. Proc. Natl. Acad. Sci. USA 80, 6192-6196.
21. Nierhaus, K.H., 1996. An elongation factor turn-on. Nature 379, 491-492.
22. Lys mutation in the mtDNA is the causal genetic lesion underlying myoclonic epilepsy and ragged-red fiber (MERRF) syndrome. Am. J. Hum. Genet. 49, 715-722.
23. Piechulla, B., Küntzel, H., 1983. Mitochondrial polypeptide elongation factor EF-Tu of Saccharomyces cerevisiae. Functional and structural homologies to Escherichia coli EF-Tu. Eur. J. Biochem. 132, 235-240.
24. Pitkänen, S., Merante, F., McLeod, D.R., Applegarth, D., Tong, T., Robinson, B.H., 1996. Familial cardiomyopathy with cataracts and lactic acidosis: a defect in complex I (NADH-dehydrogenase) of the mitochondrial respiratory chain. Pediatr. Res. 39, 513-521.
25. Rosenthal, L.P., Bodley, J.W., 1987. Purification and characterization of Saccharomyces cerevisiae mitochondrial elongation factor Tu. J. Biol. Chem. 262, 10955-10959.
26. Sambrook, J., Fritsch, E.R., Maniatis, T., 1989. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
27. Schnepf, E., Brown Jr., R.M. Jr., 1971. Origin and Continuity of Cell Organelles. In: Reinert, J., Ursprung, H. (Eds.), Springer-Verlag, New York, NY, pp. 299-322.
28. Schwartzbach, C.J., Spremulli, L.L., 1989. Bovine mitochondrial protein synthesis elongation factors. Identification and initial characterization of an elongation factor Tu-elongation factor Ts complex. J. Biol. Chem. 264, 19125-19131.
29. Schwartzbach, C.J., Spremulli, L.L., 1991. Interaction of animal mitochondrial EF-Tu. EF-Ts with aminoacyl-tRNA, guanine nucleotides and ribosomes. J. Biol. Chem. 266, 16324-16330.
30. Tubulekas, I., Hughes, D., 1993. A single amino acid substitution in elongation factor Tu disrupts interaction between the ternary complex and the ribosome. J. Bacteriol. 175, 240-250.
31. Ursin, V.M., Becker, C.K., Shewmaker, C.K., 1993. Cloning and nucleotide sequence of a tobacco chloroplast translational elongation factor EF-Tu. Plant Physiol. 101, 333-334.
32. Wallace, D.C., 1994. Mitochondrial DNA sequence variation in human evolution and disease. Proc. Natl. Acad. Sci. USA 91, 8739-8746.
33. Wells, J., Henkler, F., Leversha, M., Koshy, R., 1995. A mitochondrial elongation factor-like protein is over-expressed in tumours and differentially expressed in normal tissues. FEBS Lett. 358, 119-125.
34. Wiborg, O., Andersen, C., Knudesen, C.R., Kristensen, T.J., Clark, B.F.C., 1994. Towards an understanding of structure-function relationships of elongation factor Tu. Biotech. Appl. Biochem. 19, 3-15.
35. Wong, G.H., Goeddel, D.V., 1994. One-day Northern blotting for detection of mRNA: NDGA inhibits the induction of MnSOD mRNA by agonists of type 1 TNF receptor. Methods Enzymol. 234, 244-252.
36. Woriax, V.L., Burkhart, W.A., Spremulli, L.L., 1995. Cloning, sequence analysis and expression of mammalian mitochondrial protein synthesis elongation factor Tu. Biochim. Biophys. Acta 1264, 347-356.
37. Xin, H., Woriax, V.L., Burkhart, W., Spremulli, L.L., 1995. Cloning and expression of mitochondrial translational elongation factor Ts from bovine and human liver. J. Biol. Chem. 270, 17243-17249.
38. Yokota, T., Sugisaki, H., Takanami, M., Kaziro, Y., 1980. The nucleotide sequence of the tufA gene of Escherichia coli. Gene 12, 25-31.