Quantitative methods for measurement of transcobalamin II

Methods in Enzymology

Volumn 281, Issue , 1997, Pages 261-268

  Rothenberg, Sheldon P ^a   Quadros, Edward V ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

TRANSCOBALAMIN II;

ARTICLE; BODY FLUID; PRIORITY JOURNAL; PROTEIN BINDING; QUANTITATIVE DIAGNOSIS; RABBIT; REACTION ANALYSIS; TRANSPORT KINETICS; VITAMIN METABOLISM;

ORYCTOLAGUS CUNICULUS;

EID: 0030872463     PISSN: 00766879     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S0076-6879(97)81032-4     Document Type: Article

References (5)

1. L. Qian, E. V. Quadros, and S. P. Rothenberg, Methods Enzymol. 281, [31], 1997 (this volume).
2. E. Jacob and V. Herbert, J. Lab. Clin. Med. 86, 505 (1975).
3. E. V. Quadros, S. P. Rothenberg, Y.-Ch. E. Pan, and S. Stein, J. Biol Chem. 261,15455 (1986).
4. R. S. Yalow and S. A. Berson, J. Clin. Invest. 39, 1157 (1960).
5. Three sources of TCII can be used for the standard curve: purified TCII from human plasma, recombinant human TCII (rhTCII), or TCII in normal human serum. The concentration of the apo-TCII in serum is determined as described in the first part of this chapter and represents approximately 90% of total TCII in serum. Whereas purified human TCII and rTCII in solution have a tendency to aggregate because of their hydrophobicity, TCII in serum remains monomeric and stable and is preferable for generating the standard curve.