High-level production and purification of biologically active proteins from bacterial and mammalian cells using the tandem pGFLEX expression system

Gene

Volumn 193, Issue 2, 1997, Pages 229-237

  Manoharan, Herbert T ^a   Gallo, Jean ^a   Gulick, Andrew M ^a   Fahl, William E ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

Cyclin; Fusion protein; Glutathione S transferase

Indexed keywords

GLUTATHIONE TRANSFERASE; HYBRID PROTEIN; RECOMBINANT ENZYME; RECOMBINANT PROTEIN;

AMINO TERMINAL SEQUENCE; ANIMAL CELL; ARTICLE; BACTERIUM; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; GENE EXPRESSION REGULATION; MAMMAL CELL; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROTEIN PURIFICATION; PROTEIN SYNTHESIS; SHUTTLE VECTOR; TANDEM REPEAT;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CLONING, MOLECULAR; COS CELLS; CYTOMEGALOVIRUS; ESCHERICHIA COLI; GENES, IMMEDIATE-EARLY; GLUTATHIONE TRANSFERASE; MOLECULAR SEQUENCE DATA; PROMOTER REGIONS (GENETICS); RECOMBINANT FUSION PROTEINS;

ANIMALIA; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; MAMMALIA;

EID: 0030871621     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(97)00126-1     Document Type: Article

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