Universal template plasmid for introduction of the triple-HA epitope sequence into cloned genes

BioTechniques

Volumn 23, Issue 2, 1997, Pages 254-256

  Sato, Masa H ^a,b   Wada, Yoh ^a  

^a UNIVERSITY OF TOKYO   (Japan)

^b KYOTO UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATE; EPITOPE; HEMAGGLUTININ; OLIGONUCLEOTIDE; POLYNUCLEOTIDE 5' HYDROXYL KINASE;

AGAR GEL ELECTROPHORESIS; ARTICLE; DNA SYNTHESIS; DNA TEMPLATE; INFLUENZA VIRUS; MOLECULAR CLONING; MUTAGENESIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PHOSPHORYLATION; PLASMID; POLYMERASE CHAIN REACTION; SACCHAROMYCES CEREVISIAE; TEMPERATURE;

INFLUENZA VIRUS; SACCHAROMYCES CEREVISIAE;

EID: 0030871303     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/97232bm17     Document Type: Article

References (4)

1. Becherer, K.A., S.E. Rieder, S.D. Emr and E.J. Jones. 1996. Novel syntaxin homologue, Pep 12p, required for the sorting of lumenal hydrolases to the lysosome-like vacuole in yeast. Mol. Biol. Cell 7:579-594.
2. Clackson, T. and G. Winter. 1989. "Sticky feet" directed mutagenesis and its application to swapping antibody domains. Nucleic Acids Res. 17:10163-10170.
3. Kolodziej, P.A. and R.A. Young. 1991. Epitope tagging and protein surveillance. Methods Enzymol. 194:508-519.
4. Kunkel, T.A. 1985. Rapid and efficient site-specific mutagenesis without phenotypic selection. Proc. Natl. Acad. Sci. USA S2:488-492.