Nucleotide sequence of the gene encoding cis-biphenyl dihydrodiol dehydrogenase (bphB) and the expression of an active recombinant His-tagged bphB gene product from a PCB degrading bacterium, Pseudomonas putida OU83

FEMS Microbiology Letters

Volumn 154, Issue 2, 1997, Pages 317-324

  Khan, Ashraf A ^a   Wang, Rong Fu ^a   Nawaz, Mohamed S ^a   Cerniglia, Carl E ^a  

^a Natl Ctr Toxicological Res F   (United States)

Author keywords

cis Biphenyl dihydrodiol dioxygenase; His tagged protein; Nucleotide sequence; Polychlorinated biphenyl

Indexed keywords

DIHYDRODIOL DEHYDROGENASE; POLYCHLORINATED BIPHENYL;

AMINO ACID SEQUENCE; ARTICLE; BIODEGRADATION; CONTROLLED STUDY; GENE EXPRESSION; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PSEUDOMONAS PUTIDA;

AMINO ACID SEQUENCE; BASE SEQUENCE; HISTIDINE; MOLECULAR SEQUENCE DATA; OXIDOREDUCTASES; PSEUDOMONAS PUTIDA; RECOMBINANT PROTEINS;

BACTERIA (MICROORGANISMS); PSEUDOMONAS; PSEUDOMONAS PSEUDOALCALIGENES; PSEUDOMONAS PUTIDA;

EID: 0030870796     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(97)00348-0     Document Type: Article

References (29)

1. Furukawa, K. and Miyazaki, T. (1986) Cloning of gene cluster encoding biphenyl and chlorobiphenyl degradation of Pseudomonas pseudoalcaligenes. J. Bacteriol. 172, 1160-1164.
2. Hofer, B., Eltis, L.D., Dowling, D.N. and Timmis, K.N. (1993) Genetic analysis of a Pseudomonas locus encoding a pathway for biphenyl/polychlorinated biphenyl degradation. Gene 130, 47-55.
3. Khan, A.A. and Walia, S.K. (1991) Expression, localization, and functional analysis of polychlorinated biphenyl degradation genes cbpABCD of Pseudomonas putida. Appl. Environ. Microbiol. 57, 1325-1332.
4. Ahmad, D., Masse, R. and Sylvestre, M. (1990) Cloning and expression of genes involved in 4-chlorobiphenyl transformation by Pseudomonas testosteroni: homology to polychlorobiphenyl-degrading genes in other bacteria. Gene 86, 53-61.
5. Sylvestre, M., Hurtubise, Y., Barriault, D., Bergeron, J. and Ahmad, D. (1996) Characterization of active recombinant 2,3-dihydro-2,3-dihydroxybiphenyl dehydrogenase from Comamonas testosteroni B-356 and sequence of the encoding gene (bphB). Appl. Environ. Microbiol. 62, 2710-2715.
6. Khan, A.A., Wang, R.F., Nawaz, M.S., Cao, W.W. and Cerniglia, C.E. (1996). Purification of 2,3-dihydroxybiphenyl 1,2-dioxygenase from Pseudomonas putida OU83 and characterization of the gene (bphC). Appl. Environ. Microbiol. 62, 1825-1830.
7. Eltis, L.D., Hofmann, B., Hecht, H., Lunsdorf, H. and Timmis, K. (1993) Purification and crystallization of 2,3-dihydroxybiphenyl 1,2-dioxygenase. J. Biol. Chem. 268, 2727-2732.
8. Furukawa, K. and Arimura, N. (1987) Purification and properties of 2,3-dihydroxybiphenyl dioxygenase from chlorinated biphenyl-degrading Pseudomonas pseudoalcaligenes and Pseudomonas aeruginosa carrying the cloned bphC gene. J. Bacteriol. 169, 924-927.
9. Eltis, L.D. and Bolin, J.T. (1996) Evolutionary relationships among extradiol dioxygenases. J. Bacteriol. 178, 5930-5937.
10. Patel, T.R. and Gibson, D.T. (1974) Purification and properties of (+)-cis-naphthalene dihydrodiol dehydrogenase of Pseudomonas putida. J. Bacteriol. 119, 879-888.
11. Rogers, J.E. and Gibson, D.T. (1977) Purification and properties of cis-toluene dihydrodiol dehydrogenase from Pseudomonas putida. J. Bacteriol. 130, 1117-1124.
12. Khan, A.A., Wang, R.F., Cao, W.W., Franklin, W. and Cerniglia, C.E. (1996) Reclassification of a polycyclic aromatic hydrocarbon-metabolizing bacterium, Beijerinckia sp. strain B1, as Sphingomonas yanoikuyae by fatty acid analysis, protein pattern analysis, DNA-DNA hybridization, and 16S ribosomal DNA sequencing. Int. J. Syst. Bacteriol. 46, 466-469.
13. Gibson, D.T., Roberts, R.L., Wells, M.C. and Kobal, V.M. (1973) Oxidation of biphenyl by a Beijerinckia species. Biochem. Biophys. Res. Commun. 50, 211-219.
14. Khan, A.A. and Walia, S.K. (1989) Cloning of bacterial genes specifying degradation of 4-chlorobiphenyl from Pseudomonas putida OU83. Appl. Environ. Microbiol. 55, 798-805.
15. Maniatis, T., Fritsch, E. and Sambrook, J. (1982) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
16. Sanger, F., Nicklen, S. and Coulson, A.R. (1977) DNA sequencing with chain-terminating inhibitor. Proc. Natl. Acad. Sci. USA 74, 5463-5467.
17. Smith, P.K., Krohn, R.I., Hermanson, G.T., Mallia, A.K., Garter, F.H., Provenzano, M.D., Fujimoto, E.K., Goeke, N.M., Olson, B.J. and Klenk, D.C. (1985) Measurement of protein using bicinchoninic acid. Anal. Biochem. 150, 76-85.
18. Laemmli, U.K. (1970) Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature 227, 680-685.
19. Shine, J. and Dalgarno, L. (1975) Determination of cistron specificity in bacterial ribosomes. Nature 254, 34-38.
20. Normore, M.W. (1976) Guanine-plus-cytosine (GC) composition of the DNA of bacteria, fungi, algae and protozoa. In: Handbook of Biochemistry and Molecular Biology (Fasman, G.D., Ed.), pp. 65-240. CRC Press, Cleveland, OH.
21. Taira, K., Hirose, J., Hayashida, S. and Furukawa, K. (1992) Analysis of bph operon from the polychlorinated biphenyl-degrading strain of Pseudomonas pseudoalcaligenes KF707. J. Biol. Chem. 267, 4844-4853.
22. Fukuda, M., Yasukochi, Y., Kikuchi, Y., Nagata, Y., Kimbara, K., Horiuchi, H., Takagi, M. and Yano, K. (1994) Identification of the bphA and bphB genes of Pseudomonas sp. strain KKS102 involved in the degradation of biphenyl and polychlorinated biphenyls. Biochem. Biophys. Res. Commun. 202, 850-856.
23. Masai, E., Yamada, A., Healy, J., Hatta, T., Kimbara, K., Fukuda, M. and Yano, K. (1995) Characterization of biphenyl catabolic genes of gram-positive polychlorinated biphenyl degrader Rhodococcus sp. strain RHA1. Appl. Environ. Microbiol. 61, 2079- 2085.
24. Asturias, J.A., Eltis, L.D., Prucha, M. and Timmis, K.N. (1994) Analysis of three 2,3-dihydroxybiphenyl 1,2-dioxygenases found in Rhodococcus globerulus P6. J. Biol. Chem. 269, 7807-7815.
25. Neidle, E., Hartnett, C., Ornston, L., Bairoch, A., Rekik, M. and Harayama, S. (1992) Cis-diol dehydrogenases encoded by the TOL pWWO plasmid xylL gene and the Acinetobacter calcoaceticus chromosomal benD gene are members of the short-chain alcohol dehydrogenase superfamily. Eur. J. Biochem. 204, 113-120.
26. Axcell, B.C. and Geary, P.J. (1973) The metabolism of benzene by bacteria. Purification and some properties of the enzyme cis-1,2-dihydroxycyclohexa-3,5-diene (nicotinamide adenine dinucleotide) oxidoreductase (cis-benzene glycol dehydrogenase). Biochem. J. 136, 927-934.
27. +) oxidoreductase (decarboxylating). J. Biol. Chem. 247, 4960-4965.
28. Morris, H.R., William, D.H., Midwinter, G.G. and Hartley, B.S. (1974) A mass-spectrometric sequence study of the enzyme ribitol dehydrogenase from Klebsiella aerogenes. Biochem. J. 141, 701-713.
29. Baker, M.E. (1990) Sequence similarity between Pseudomonas dihydrodiol dehydrogenase, part of the gene cluster that metabolizes polychlorinated biphenyls, and dehydrogenases involved in metabolism of ribitol and glucitol and synthesis of antibiotics and 17β-oestradiol testosterone and corticosterone. Biochem. J. 267, 839-841.