Optimisation of the PEG reconcentration procedure for virus detection by cell culture or genomic amplification

Water Science and Technology

Volumn 35, Issue 11-12, 1997, Pages 455-459

  Vilagines Ph ^a   Suarez, A ^b   Sarrette, B ^a   Vilagines R ^a  

^a Centre de Recherche et de Contrôle de l'Eau de Paris   (France)

^b UNIVERSIDAD NACIONAL DE TUCUMÁN   (Argentina)

Author keywords

Drinking water; PEG concentration; Poliovirus; Rotavirus; RT PCR

Indexed keywords

ADSORPTION; BIOASSAY; CELL CULTURE; MICROBIOLOGY; POLYETHYLENE GLYCOLS; POTABLE WATER; RNA; VIRUSES;

ELUTION; GENOMIC AMPLIFICATION; POLIOVIRUS; ROTAVIRUS; VIRUS DETECTION;

WATER TREATMENT;

DRINKING WATER; MACROGOL 6000; SODIUM CHLORIDE; TAP WATER; VIRUS RNA;

ADSORPTION; CELL CULTURE; CENTRIFUGATION; CONFERENCE PAPER; COXSACKIE VIRUS; EXTRACTION; GENE AMPLIFICATION; POLIOMYELITIS VIRUS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; ROTAVIRUS; TEMPERATURE; TIME; VIRUS DETECTION; WATER CONTAMINATION; WATER QUALITY;

COXSACKIEVIRUS; POLIOVIRUS; RNA VIRUSES; ROTAVIRUS; ROTAVIRUS A;

EID: 0030869513     PISSN: 02731223     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S0273-1223(97)00303-X     Document Type: Conference Paper

References (6)

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2. Baron, A. L., Olshevesky, C. and Cohen, M. N. (1970). Characteristics of the BGM line of cells from African Green Monkey Kidney. Arch. Ges. Virusforshung, 32, 389-392.
3. Katzenelson, E., Fattal B. and Hostovesky, T. (1976). Organic flocculation: an efficient second step concentration method for the detection of viruses in tap water. Appl. Environ. Microbiol., 32, 638-639.
4. Kopecka, H., Dubrou, S., Prevot, J., Marechal, J. and Lopez-Pila, J. M. (1993). Detection of naturally occuring enteroviruses in waters by reverse transcription, polymerase chain reaction and hybridisation. Appl. Environ. Microbiol., 59, 1213-1219.
5. Schwab, K. J. S. De Leon, R. and Sobsey, M. D. (1993). Development of PCR methods for enteric virus detection in water. Wat. Sci. Tech., 27(3-4), 211-218.
6. Vilaginès, Ph., Sarrette, B., Husson, G. and Vilaginès, R. (1993). Glass wool for virus concentration from water at ambient pH levels. Wat. Sci. Tech., 27(3-4), 299-306.