Activity and inhibition of 3-beta-hydroxysteroid dehydrogenase/delta-5-4-lsomerase in human skin

Skin Pharmacology and Physiology

Volumn 10, Issue 3, 1997, Pages 160-168

  Tóth, István ^a   Szécsi, Mihály ^a   Julesz, János ^a   Faredin, Imre ^a  

^a UNIVERSITY OF SZEGED   (Hungary)

Author keywords

Androgen activity; Enzymes; Human skin; Inhibitors

Indexed keywords

ANDROGEN; ANDROSTENEDIONE; CYPROTERONE ACETATE; EPOSTANE; ISOMERASE; OXIDOREDUCTASE; PRASTERONE;

ANDROGEN SYNTHESIS; ARTICLE; CONTROLLED STUDY; DERMIS; ENZYME ACTIVITY; ENZYME INHIBITION; FEMALE; HUMAN; HUMAN CELL; HUMAN TISSUE; INGUINAL REGION; MALE; MICHAELIS CONSTANT; PERINEUM; PRIORITY JOURNAL; RADIOENZYMATIC ASSAY; SKIN CELL;

ADULT; AGED; ANDROSTENOLS; AZASTEROIDS; CYPROTERONE ACETATE; DEHYDROEPIANDROSTERONE; DIHYDROTESTOSTERONE; ENZYME INHIBITORS; FEMALE; HUMANS; KINETICS; MALE; MIDDLE AGED; MULTIENZYME COMPLEXES; PROGESTERONE REDUCTASE; SKIN; STEROID ISOMERASES; STEROIDS;

EID: 0030868533     PISSN: 16605527     EISSN: 16605535     Source Type: Journal    
DOI: 10.1159/000211481     Document Type: Article

References (53)

1. Faredin I, Webb JL, Julesz M: In vitro metabolism of dehydroepian-drosterone in human skin, 2nd Int Congr Hormonal Steroids, Milan, Excerpta Med Int Congr Ser 1966; 111:278.
2. Faredin I, Webb JL, Julesz M: The in vitro metabolism of dehydro-epiandrosterone in human skin, Acta Med Acad Sci Hung 1967.23: 169-180.
3. l4C-dehydroepian-drosterone by human male pubic skin, Eur J Steroids 1967;2:223-242.
4. 14C]5-androstcnc-3ß-17ß-diol by normal human skin in vitro, Acta Med Acad Sei Hung 1975:32:139-152.
5. Gallegos AJ, Berliner DL: Transformation and conjugation of dehy-droepiandrosterone by human skin, J Endocrinol 1967;27:1214-1218.
6. l4C]dehydroepiandroste-rone sulphate by normal human female skin slices, J Endocrinol 1968; 41:295-296.
7. Berliner DL, Pasqualini JR, Gallegos AJ: The formation of water soluble steroids by human skin, J Invest Dermatol 1968;50:220-224.
8. l4C]dehydroepiandroste-rone into 7-oxygenatcd derivatives by normal human male and female skin tissue, J Invest Dermatol 1969; 52:357-361.
9. Rongone EL: Testosterone metabolism by human male mammary skin, Steroids 1966;7:489-504.
10. Wotiz HH, Mescon H, Doppel H, Lemon HM: The in vitro metabolism of testosterone by human skin, J Invest Dermatol 1956;38:113-120.
11. 14C in human skin, Biochemistry NY 1968;7: 24-32.
12. l4C]dchydro-epiandrosterone into androst-4-cne-3.17-dione, testosterone and andros-tcrone by normal human male and female skin slices, Acta Med Acad Sei Hung 1970;27:95-102.
13. Julesz M, Faredin I, Toth I: Steroids in Human Skin, Budapest, Akade-miai Kiado, 1971. pp 62-84.85-96.
14. Töth I, Faredin I: Metabolism and concentration of androgenic steroids in abdominal skin of hirsute women with adrenogenital syndrome, Acta Med Acad Sei Hung 1984;41:7-18.
15. Faredin I, Töth I: Metabolism and concentration of androgenic steroids in the abdominal skin of women with idiopathic hirsutism, Acta Med Acad Sei Hung 1984.41:19-34.
16. 5-3-hydroxide steroids to a, ß unsaturated ketones, Science 1951:113: 490-491.
17. Ishii-Ohba H, Inano H, Tamaoki BI: Testicular and adrenal 3p-hydroxy-5-ene-steroid dehydrogenase and 5-ene-4-ene isomerase, J Steroid Biochem 1987;27:775-779.
18. 4-isomcrase gene and its expression in mammalian cells, J Biol Chem 1990;265:20469-20475.
19. Luu-The V, Takahashi M, Labrie F: Differential inhibition of dehydrogenase and 5-ene-*4-ene-isomerase activities of purified 3P-hydroxystc-roid dehydrogenase: Evidence for two distinct sites, J Steroid Biochem Mol Biol 1991;40:545-548.
20. Hammerstein J, Meckies J, Leo-Rossberg I, Moltz L, Zielske F: Use of cyproterone acetate in the treatment of acne, hirsutism and virilism, J Steroid Biochem 1975;6:827-836.
21. Julesz J, Faredin I, Toth I, Uszle FA: Cyproterone acetate treatment of females with hyperandrogenism, Endocrinologie 1981;78:168-174.
22. Scultety S, Oszlanezy J, Faredin I, Toth I: High-dose ketoconazole treatment in advanced prostatic cancer, Int Urol Nephrol 1988;20: 383-393.
23. Faredin I, Toth I, Oszlanezy J, Scultety S: Activity and inhibition of 5a-reductase of human prostate, Magyar Urol 1991;3:289-297
24. Faredin I, Toth I, Oszlanczy J, Scultety S: In vitro study of rat prostate 5a-reductase activity and its inhibition, Int Urol Nephrol 1992;24: 145-154.
25. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ: Protein measurement with the Folin phenol reagent, J Biol Chem 1951;193:265-275.
26. Faredin I, Toth I: Steroid studies on florisil adsorbent column, III, Chromatography of steroids on ‘Nimco' florisil column, Acta Med Acad Sci Hung 1964;20:185-192.
27. l4C double isotope dilution method for the identification of steroids, Izotoptcchnika, Diagnosztika 1990; 33:249-258.
28. Lineweaver H, Burk D: The determination of enzyme dissociation constants, J Am Chem Soc 1934;56: 658-666.
29. Thomas JL, Berko EA, Fanstino A, Myers RP, Strickler RC: Human placental 3p-hydroxy-5-ene-steroid dehydrogenase and steroid 5->4-ene-isomerase purification from mi-crosomes, infstrate kinetics, and inhibition by product steroids, J Steroid Biochem 1988;31:785-793.
30. Deslypere JP, Sayed A, Verdonck L, Vermeulen A: Androgen concentrations in sexual and non-sexual skin as well as in striated muscle in man, J Steroid Biochem 1980; 13:1455-1458.
31. Töth I, Faredin I: Concentrations of androgens and Cirsteroid sulphates in abdominal skin of healthy women and men, Acta Med Acad Sci Hung 1985;42:13-20.
32. Töth I, Faredin I: Steroids excreted by human skin, I, Ci9-stcroids in axillary hair, Acta Med Acad Sci Hung 1983;40:139-145.
33. Töth I, Faredin I: Steroids excreted by human skin, II, Cu-steroid sulphates in human axillary sweat, Acta Med Acad Sci Hung 1985;42: 21-28.
34. Faredin I, Töth I: Formation and concentration of dehydroepiandros-terone sulphate in the abdominal skin of healthy individuals and patients with endocrine disease, Acta Med Acad Sci Hung 1983.40:183-193.
35. l4C]androst-4-ene-3.17-dione by normal and hirsute female skin, Int J Dermatol 1970;9:147-152.
36. 3H]-tcstos-terone by skin from men (abstract), Endocrinology 1966;35:XIX.
37. Sansone G, Rcisner RM: Differential rates of conversion of testosterone to dihydrotestosterone in acne and in normal human skin: A possible pathogenic factor in acne, J Invest Dermatol 1971;56:366-372.
38. Oertel GW, Treiber L: Metabolism and excretion of C19- and Cg-stc-roids by human skin, Eur J Biochem 1969;7:234-238.
39. Voigt W, Fernandez EP, Hsia IL: Transformation of testosterone into 17ß-hydroxy-5a-androstan-3-onc by microsomal preparations of human skin, J Biol Chem 1970:245:5594-5599.
40. Voigt W, Hsia IL: Further studies on testosterone 5a-reductase of human skin, J Biol Chem 1973.248:4280-4285.
41. Hsia IL, Voigt W: Testosterone 5a-reductase of human skin; in Läslö FA (ed): Recent Results in Peptide Hormone and Androgenic Steroid Research, Budapest, Akademiai Kiado, 1979. pp 209-214.
42. Kutten F, Mauvais-Jarvis P: Testosterone 5a-reduction in the skin of normal infjects and of patients with abnormal sex development, Acta Endocrinol (Copenhagen) 1975;79: 164-176.
43. Itami S, Takayasu S: Activity of 17ß-hydroxysteroid dehydrogenase in various tissues of human skin, Br J Dermatol 1981;105:693-699.
44. Thcintz GE, Steimer TJ, Siromenko PG: Developmental pattern of 17p-hydroxysteroid dehydrogenase and 5a-reductase activities in the foreskin of boys from birth to eight years of age, Horm Res 1989;32:124-129.
45. Kim MH, Herrmann WL: In vitro metabolism of dehydroepiandroste-rone sulphate in foreskin, abdominal skin and vaginal mucosa, J Clin Endocrinol Metab 1968.28:187-191.
46. Hay JB, Hodgins MB: The androgen metabolism of human skin, J Endocrinol 1971;51:XXIII-XXIV.
47. Hay JB, Hodgins MB: Metabolism of androgens in vitro by human facial and axillary skin, J Endocrinol 1973;59:475-486.
48. Hay JB: A study of the in vitro metabolism of androgens by human scalp and pubic skin, Br J Dermatol 1977;97:237-246.
49. Hay JB, Hodgins MB: Distribution of androgen metabolizing enzymes in isolated tissues of human forehead and axillary skin, J Endocrinol 1978;79:29-39.
50. Thormiton MJ, Laing J, Hamada K, Messenger AG, Randall VA: Differences in testosterone metabolism by beard and scalp hair follicle dermal papilla cells, Clin Endocrinol 1993; 39:633-639.
51. Creange JE, Anzalone AJ, Potts GO, Schane HP: WIN 32729 a new, potent interceptive agent in rats and rhesus monkeys, Contraception 1981;24:289-299.
52. Pattinson NI, Webster MA, Phipps IL, Anderson ABM, Gillmer MDG: Inhibition of 3ß-hydroxysteroid dehydrogenase activity in first and second trimester human pregnancy and the luteal phase using epostane, Fer-til Steril 1985;42:875-881.
53. Webster MA, Gillmer MG: Induction of abortion in early first trimester human pregnancy using epostane, Br J Obstct Gynaecol 1989;96: 340-345.