Synthetic potential of cloned fucosyl-transferase III and VI

Bioorganic and Medicinal Chemistry Letters

Volumn 7, Issue 19, 1997, Pages 2447-2450

  Baisch, Gabi ^a   Öhrlein, Reinhold ^a   Katopodis, Andreas ^a   Streiff, Markus ^a   Kolbinger, Frank ^a  

^a NOVARTIS PHARMA AG   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

FUCOSYLTRANSFERASE; OLIGOSACCHARIDE;

ARTICLE; ENZYME CONFORMATION; ENZYME SPECIFICITY; ENZYME SUBSTRATE COMPLEX; ENZYME SYNTHESIS; FUCOSYLATION; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; SEQUENCE HOMOLOGY;

EID: 0030868168     PISSN: 0960894X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0960-894X(97)00429-0     Document Type: Article

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14. 15 The clear solution is treated with 166 μl (6U/ml) of a fucosyl-transferase III (or fucosyl-transferase VI, correspondingly) solution and 2 μl (∼34 U) calf intestine alkaline phosphatase (Boehringer no. 108146, 7500 U/498 ml). The mixture is incubated at 37°C with stirring for 4 days. The resulting turbid solution is then centrifuged and the supernatant passed over a short C-18 reversed phase column, lyophilized and subsequently purified on silica gel (eluent: methylene chloride - methanol - water / 10 - 4 - 0.5, vol - vol -vol). A final lyophilization of the tetrasaccharide containing fractions from dioxane yields 9.9 mg (83%) of tetrasaccharide 2c as a white powder. The incubations with the parent GDP-fucose donor can be terminated after one day of incubating.
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