Crosslinked plasmalemmal cholesterol is sequestered to caveolae: Analysis with a new cytochemical probe

Journal of Histochemistry and Cytochemistry

Volumn 45, Issue 9, 1997, Pages 1197-1205

  Fujimoto, Toyoshi ^a   Hayashi, Masami ^b   Iwamoto, Machiko ^b   Ohno Iwashita, Yoshiko ^b  

^a GUNMA UNIVERSITY   (Japan)

^b TOKYO METROPOLITAN INSTITUTE OF GERONTOLOGY   (Japan)

Author keywords

Caveolae; Cholesterol; Electron microscopy; Freeze fracture; Histochemistry; Plasma membrane; toxin

Indexed keywords

AVIDIN; CHOLESTEROL; COLLOIDAL GOLD; DIGITONIN; FILIPIN; FLUORESCEIN; LIPID BINDING PROTEIN; STREPTAVIDIN; TOXIN;

ANIMAL CELL; ARTICLE; CELL MEMBRANE; CROSS LINKING; IMMUNOCYTOCHEMISTRY; LIPID COMPOSITION; MEMBRANE STRUCTURE; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN LIPID INTERACTION;

ANIMALIA;

EID: 0030865913     PISSN: 00221554     EISSN: None     Source Type: Journal    
DOI: 10.1177/002215549704500903     Document Type: Article

References (39)

1. Andrews LD, Cohen AI (1979) Freeze-fracture evidence for the presence of cholesterol in particle-free patches of basal disks and the plasma membrane of retinal rod outer segments of mice and frogs. J Cell Biol 81:215-228
2. Bolard J (1986) How do the polyene macrolide antibiotics affect the cellular membrane properties? Biochim Biophys Acta 864:257-304
3. Brown DA, Rose JK (1992) Sorting of GPI-anchored proteins to glycolipid-enriched membrane subdomains during transport to the apical cell surface. Cell 68:533-544
4. Clark MR, Mel S, Lupu F, Friend DS (1987) Influence of the membrane undercoat on filipin perturbation of the red blood cell membrane. Exp Cell Res 171:321-330
5. Elias PM, Friend DS, Goerke J (1979) Membrane sterol heterogeneity. Freeze-fracture detection with saponins and filipin. J Histochem Cytochem 27:1247-1260
6. Elias PM, Goerke J, Friend DS (1978) Freeze-fracture identification of sterol-digitonin complexes in cell and liposome membranes. J Cell Biol 78:577-596
7. Fra AM, Williamson E, Simons K, Parton RG (1994) Detergent-insoluble glycolipid microdomains in lymphocytes in the absence of caveolae. J Biol Chem 269:30745-30748
8. Fujimoto T (1996) GPI-anchored proteins, glycosphingolipids, and sphingomyelin are distributed diffusely in untreated cells and sequestered to caveolae only after crosslinking. J Histochem Cytochem 44:929-941
9. Fujimoto T, Aoki T, Hagiwara H, Hayashi M, Ohno-Iwashita Y (1996) Analysis of plasmalemmal organization by a new cholesterol probe. Histochemisty and Cytochemistry 1996. Proc Xth Int Congr Histochem Cytochem. Saito T, ed. Japan Society of Histochemistry and Cytochemistry, 170-171
10. Fujimoto T, Miyawaki A, Mikoshiba K (1995) Inositol 1,4,5-trisphosphate receptor-like protein in plasmalemmal caveolae is linked to actin filaments. J Cell Sci 108:7-15
11. Fujimoto T, Ogawa K (1983) Uneven formation of filipin-sterol complexes in frog urinary bladder epithelium. Acta Histochem Cytochem 16:513-521
12. Gorodinsky A, Harris DA (1995) Glycolipid-anchored proteins in neuroblastoma cells form detergent-resistant complexes without caveolin. J Cell Biol 129:619-627
13. Jones HM, Miyai K (1981) Ultrastructural localization of cholesterol by enzyme histochemistry. Histochem J 13:1017-1028
14. Kurzchalia TV, Harmann E, Dupree P (1995) Guilt by insolubility - does a protein's detergent insolubility reflect a caveolar location? Trends Cell Biol 5:187-189
15. Lange YH, Matthies H, Steck TL (1984) Cholesterol oxidase susceptibility of the red cell membrane. Biochim Biophys Acta 769:551-562
16. Mayor S, Rothberg K, Maxfield FR (1994) Sequestration of GPI-anchored proteins in caveolae triggered by crosslinking. Science 264:1948-1951
17. McGookey R, Vassalli R, Anderson RGW (1983) Filipin-cholesterol complexes form in uncoated vesicle membrane derived from coated vesicles during receptor-mediated endocytosis of low density lipoprotein. J Cell Biol 96:1273-1278
18. Murata M, Peranen J, Schreiner R, Wieland F, Kurzchalia TV, Simons K (1995) VIP21/caveolin is a cholesterol-binding protein. Proc Natl Acad Sci USA 92:10339-10343
19. Ohno-Iwashita Y, Iwamoto M, Ando S, Mitsui K, Iwashita S (1990) A modified θ-toxin produced by limited proteolysis and methylation: a probe for the functional study of membrane cholesterol. Biochim Biophys Acta 1023:441-448
20. Ohno-Iwashita Y, Iwamoto M, Mitsui K, Ando S, Iwashita S (1991) A cytolysin, θ-toxin, preferentially binds to membrane cholesterol surrounded by phospholipids with 18-carbon hydrocarbon chains in cholesterol-rich region. J Biochem 110: 369-375
21. Ohno-Iwashita Y, Iwamoto M, Mitsui K, Ando S, Nagai Y (1988) Protease-nicked θ-toxin of Clostridium perfringens, a new membrane probe with no cytolytic effect, reveals two classes of cholesterol as toxin-binding sites on sheep erythrocytes. Eur J Biochem 176:95-101
22. Ohno-Iwashita Y, Iwamoto M, Mitsui K, Kawasaki H, Ando S (1986) Cold-labile hemolysin produced by limited proteolysis of θ-toxin from Clostridium perfringens. Biochemistry 25: 6048-6053
23. Pauli BU, Weinstein RS, Sohle LW, Alroy J (1977) Freeze-fracture of monolayer cultures. J Cell Biol 72:763-769
24. Pelletier R-M, Vitale ML (1994) Filipin vs enzymatic localization of cholesterol in guinea pig, mink, and mallard duck testicular cells. J Histochem Cytochem 42:1539-1554
25. Pinto da Silva P, Kan FWK (1984) Label-fracture: a method for high resolution labeling of cell surfaces. J Cell Biol 99:1156-1161
26. Rothberg K, Heuser JE, Donzell WC, Ying Y, Glenney JR, Anderson RGW (1992) Caveolin, a protein component of caveolae membrane coats. Cell 68:673-682
27. Rothberg K, Ying Y, Kamen BA, Anderson RGW (1990) Cholesterol controls the clustering of the glycophospholipid-anchored membrane receptor for 5-metyltetrahydrofolate. J Cell Biol 111:2931-2938
28. Rutter G, Bohn W, Hohenberg H, Mannweiler K (1985) Preparation of apical plasma membranes from cells grown on coverslips. Electron microscopic investigations of the protoplasmic surface. Eur J Cell Biol 39:443-448
29. Sargiacomo M, Sudol M, Tang Z, Lisanti MP (1993) Signal transducing molecules and glycosyl-phosphatidylinositol-linked proteins form a caveolin-rich insoluble complex in MDCK cells. J Cell Biol 122:789-807
30. Schnitzer JE, Oh P, Pinney E, Allard J (1994) Filipin-sensitive caveolae-mediated transport in endothelium: reduced transcytosis, scavenger endocytosis, and capillary permeability of select macromolecules. J Cell Biol 127:1217-1232
31. Schnitzer JE, McIntosh DP, Dvorak AM, Liu J, Oh P (1995) Separation of caveolae from associated microdomains of GPI-anchored proteins. Science 269:1435-1439
32. Severs NJ (1995) Lipid localization by membrane perturbation: a cautionary tale. In Severs NJ, Shotton DM, eds. Rapid Freezing, Freeze-Fracture, and Deep Etching. New York, Wiley-Liss, 225-234
33. Severs NJ, Simons HL (1983) Failure of filipin to detect cholesterol-rich domains in smooth muscle plasma membrane. Nature 303:637-638
34. Severs NJ, Warren RC, Barnes SH (1981) Analysis of membrane structure in the transitional epithelium of the rat urinary bladder. 3. Localization of cholesterol using filipin and digitonin. J Ultrastruct Res 77:160-188
35. Simionescu N, Lupu F, Simionescu M (1983) Rings of membrane sterols surround the openings of vesicles and fenestrae in capillary endothelium. J Cell Biol 97:1592-1600
36. Simons HL, Severs NJ (1986) Plasmalemmal vesicles and the effects of sterol-binding agents in rabbit aortic endothelium. J Cell Sci 83:141-153
37. Smart EJ, Ying Y-S, Conrad PA, Anderson RGW (1994) Caveolin moves from caveolae to the Golgi apparatus in response to cholesterol oxidation. J Cell Biol 127:1185-1197
38. Yeagle PL (1985) Cholesterol and the cell membrane. Biochim Biophys Acta 822:267-287
39. Yuspa SH, Hawley-Nelson P, Koehler B, Stanley JR (1980) A survey of transformation markers in differentiating epidermal cell lines in culture. Cancer Res 40:4694-4703