Estimation of the section thickness and optical disector height with a simple calibration method

Journal of Microscopy

Volumn 187, Issue 2, 1997, Pages 104-109

  Korkmaz, A ^a   Tumkaya L ^a  

^a ONDOKUZ MAYIS UNIVERSITY SCHOOL OF MEDICINE   (Turkey)

Author keywords

Calibration; Fine focus knob; Optical disector height; Section thickness; Stereology; Thickness sampling fraction

Indexed keywords

CALIBRATION METHOD; FINE FOCUS; FINE FOCUS KNOB; OPTICAL DISECTOR HEIGHT; OPTICAL-; SECTION THICKNESS; SHAFT ENCODERS; SIMPLE++; STEREOLOGY; THICKNESS SAMPLING FRACTION;

CALIBRATION;

ARTICLE; CALIBRATION; HYSTERESIS; OPTICS; PRIORITY JOURNAL; SAMPLING; TISSUE SECTION;

EID: 0030865255     PISSN: 00222720     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-2818.1997.2180777.x     Document Type: Article

References (19)

1. Bedi, K.S. (1987) A simple method of measuring the thickness of semi-thin and ultra-thin sections. J. Microsc. 148, 107-111.
2. Bjung. R. & Gundersen, H.J.G. (1993) Estimate of the total number of neurons and glial and endothelial cells in the rat spinal cord by means of the optical disector. J. Comp. Neurol. 328, 406-414.
3. Cruz-Orive. L.M. (1987) Particle number can be estimated using a disector of unknown thickness: the selector. J. Microsc. 145, 121-142.
4. Evans, S.M. & Howard, V. (1989) A simplification of the step method for estimating mean section thickness. J. Microsc. 154, 289-293.
5. Gundersen, H.J.G. (1986) Stereology of arbitrary particles. A review of unbiased number and size estimators and the presentation of some new ones, in memory of William R. Thompson. J. Microsc. 143, 3-45.
6. Gundersen, H.J.G., Andersen, B.S. & Floe, H. (1983) Estimation of section thickness unbiased by cutting-deformation. J. Microsc. 131, RP3-RP4.
7. Gundersen, H.J.G., Bagger, P., Bendtsen, T.F., Evans, S.M., Korbo, L., Marcussen, N., Moller, A., Nielsen, K., Nyengaard, J.R., Pakkenberg, B., Sorensen, F.B., Vesterby, A. & West, M.J. (1988) The new stereological tools: disector. fractionator. nucleator and point sampled intercepts and their use in pathological research and diagnosis. APMIS. 96, 857-881.
8. Guntinas Lichius, O., Mockenhaupt, J., Stennert, E. & Neiss, W.F. (1993) Simplified nerve cell counting in the rat brainstem with the physical disector using a drawing-microscope. J. Microsc. 172, 177-180.
9. Heinsen, H., Henn, R., Eisenmenger, W., Götz, M., Bohl, J., Bethke, B., Lockemann, U. & Püschel, K. (1994) Quantitative investigations on the human entorhinal area: left-right asymmetry and agerelated changes. Anat. Embryol. 190, 181-194.
10. Holm, I.E. & West, M.J. (1994) Hippocampus of the domestic pig: a stereological study of subdivisional volumes and neuron numbers. Hippocampus. 4, 115-126.
11. Janson, A.M. & Moller, A. (1993) Chronic nicotine treatment counteracts nigral cell loss induced by a partial mesodiencephalic hemitransection: an analysis of the total number and volume of neurons and glia in the substantia nigra of the male rat. Neuroscience, 57, 931-941.
12. Laroye, G.J. & Taylor, W.B. (1992) An acoustic depth alarm device to monitor vertical translation of the microscope stage: a practical optical disector. selector and unbiased brick for routine light microscopy. J. Microsc. 167, 279-286.
13. Larsen, J.O., Tandrup, T. & Brændgaard, H. (1994) The volume of Purkinje cells decreases in the cerebellum of acrylamide-intoxicated rats, but no cells are lost. Acta Neuropathol. 88, 307-312.
14. Pakkenberg, B. & Gundersen, H.J.G. (1989) New stereological method for obtaining unbiased and efficient estimates of total nerve cell number in human brain areas. APMIS, 97, 677-681.
15. Uylings, H.B.M., van Eden, C.G. & Hofman, M.A. (1986) Morphometry of size/volume variables and comparison of their bivariate relations in the nervous system under different conditions. J. Neurosci. Meth. 18, 19-37.
16. West, M.J. & Gundersen, H.J.G. (1990) Unbiased stereological estimation of the number of neurons in the human hippocampus. J. Comp. Neurol. 296, 1-22.
17. West, M.J., Slomianka, L. & Gundersen, H.J.G. (1991) Unbiased stereological estimation of the total number of neurons in the subdivisions of the rat hippocampus using the optical fractionator. Anat. Rec. 231, 482-497.
18. Williams, R.W. & Rakic, P. (1988) Three dimensional counting: an accurate and direct method to estimate numbers of cells in sectioned material. J. Comp. Neurol. 278, 344-352. (Erratum and addendum: J. Comp. Neurol (1989) 281, 335.)
19. Williams, R.W. & Rakic, P. (1988) Three dimensional counting: an accurate and direct method to estimate numbers of cells in sectioned material. J. Comp. Neurol. 278, 344-352. (Erratum and addendum: J. Comp. Neurol (1989) 281, 335.)