New retroviral vector, CA1, to identify and select for cells expressing an inserted gene in vitro and in vivo

Gene

Volumn 196, Issue 1-2, 1997, Pages 187-189

  Abram, Clare L ^a,b   Page, Martin J ^c   Edwards, Paul A W ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^b SUGEN INC   (United States)

^c GLAXOSMITHKLINE   (United Kingdom)

Author keywords

galactosidase; Geo; IRES; Marker gene

Indexed keywords

BETA GALACTOSIDASE; MESSENGER RNA; PHOSPHOTRANSFERASE;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; CELL GROWTH; CELL TRANSFORMATION; CELLULAR DISTRIBUTION; CLONING VECTOR; ENCEPHALOMYOCARDITIS VIRUS; ENZYME ACTIVITY; GENE INSERTION; IN VITRO STUDY; MAMMARY GLAND; MARKER GENE; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; ONCOGENE SRC; OPEN READING FRAME; PRIORITY JOURNAL; RETROVIRUS; STAINING;

ANIMALS; BETA-GALACTOSIDASE; GENETIC VECTORS; KANAMYCIN KINASE; MAMMARY GLANDS, ANIMAL; MICE; MOLECULAR SEQUENCE DATA; ONCOGENE PROTEIN PP60(V-SRC); RECOMBINANT PROTEINS; RETROVIRIDAE; RIBOSOMES; SELECTION (GENETICS);

ANIMALIA; ENCEPHALOMYOCARDITIS VIRUS; UNIDENTIFIED RETROVIRUS;

EID: 0030864770     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(97)00226-6     Document Type: Article

References (13)

1. Boris-Lawrie, K.A., Temin, H.M., 1993. Recent advances in retrovirus vector technology. Curr. Opin. Gen. Dev. 3, 102-109.
2. Bradbury, J.M., Edwards, P.A.W., 1988. Changes in in vitro growth behaviour of the mammary epithelial cell line NMuMG caused by the v-fos oncogene. Int. J. Cancer 42, 923-929.
3. Edwards, P.A.W., Abram, C.L., Bradbury, J.M., 1996. Genetic manipulation of mammary epithelium by transplantation. J. Mammary Gland Biol. Neoplasia 1, 75-89.
4. Emerman, M., Temin, H.M., 1984. Genes with promoters in retrovirus vectors can be independently suppressed by an epigenetic mechanism. Cell 39, 459-467.
5. Friedrich, F., Soriano, P., 1991. Promoter traps in embryonic stem cells: a genetic screen to identify and mutate developmental genes in mice. Genes Dev. 5, 1513-1523.
6. Ghattas, I.R., Sanes, J.R., Majors, J.E., 1991. The Encephalomyocarditis virus internal ribosome entry site allows efficient coexpression of two genes from a recombinant provirus in cultured cells and in embryos. Mol. Cell. Biol. 11, 5848-5859.
7. Kaminski, A., Howell, M.T., Jackson, R.J., 1990. Initiation of encephalomyocarditis virus RNA translation: the authentic initiation site is not selected by a scanning mechanism. EMBO J. 9, 3753-3759.
8. Lillien, L., 1995. Changes in retinal cell fate induced by overexpression of EGF receptor. Nature 377, 158-162.
9. Markowitz, D., Goff, S., Bank, A., 1988. A safe packaging line for gene transfer: Separating viral genes on two different plasmids. J. Virol. 62, 1120-1124.
10. Miller, A.D., 1992. Retroviral vectors. Curr. Top. Microbiol. Immunol. 158, 1-24.
11. Morgenstern, J.P., Land, H., 1990. Advanced mammalian gene transfer: high titre retroviral vectors with multiple drug selection markers and a complementary helper-free packaging line. Nucleic Acids Res. 18, 3587-3596.
12. Mountford, P.S., Smith, A.G., 1995. Internal ribosome entry sites and dicistronic RNAs in mammalian transgenesis. Trends Genet. 11, 179-184.
13. Yenofsky, R.L., Fine, M., Fellow, J.W., 1990. A mutant neomycin phosphotransferase II gene reduces the resistance of transformants to antibiotic selection pressure. Proc. Natl. Acad. Sci. USA 87, 3435-3439.