Regional, developmental and interspecies expression of the four NMDAR2 subunits, examined using monoclonal antibodies

Molecular Brain Research

Volumn 51, Issue 1-2, 1997, Pages 23-32

  Laurie, David J ^a,b   Bartke, Ilse ^b   Schoepfer, Ralf ^a,b   Naujoks, Kurt ^b   Seeburg, Peter H ^a  

^a UNIVERSITY OF HEIDELBERG   (Germany)

^b F HOFFMANN LA ROCHE LTD   (Switzerland)

Author keywords

Brain expression; Development; Frog subunit; Human subunit; Immunoblot; Mouse monoclonal antibody; Mouse subunit; NMDAR2 subunit; Rabbit subunit; Rat subunit

Indexed keywords

MONOCLONAL ANTIBODY; N ACETYL BETA GLUCOSAMINIDASE; N METHYL DEXTRO ASPARTIC ACID RECEPTOR; RECEPTOR SUBUNIT;

ALTERNATIVE RNA SPLICING; AMINO ACID SEQUENCE; ARTICLE; BRAIN STEM; CEREBELLUM; DEGLYCOSYLATION; FOREBRAIN; IMMUNOBLOTTING; MESENCEPHALON; MOLECULAR WEIGHT; NEUROTRANSMISSION; NONHUMAN; PRIORITY JOURNAL; RAT; THALAMUS;

AGING; ANIMALS; ANTIBODIES, MONOCLONAL; BRAIN; CELL LINE; GENE EXPRESSION REGULATION; GENE EXPRESSION REGULATION, DEVELOPMENTAL; HUMANS; MICE; ORGAN SPECIFICITY; RABBITS; RANIDAE; RATS; RECEPTORS, N-METHYL-D-ASPARTATE; RECOMBINANT PROTEINS; SPECIES SPECIFICITY; TRANSFECTION;

ANURA; ORYCTOLAGUS CUNICULUS;

EID: 0030864283     PISSN: 0169328X     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0169-328X(97)00206-4     Document Type: Article

References (57)

1. Akbarian S., Sucher N.J., Bradley D., Tafazzoli A., Trinh D., Hetrick W.P., Potkin S.G., Sandman C.A., Bunney W.E., Jones E.G. Selective alterations in gene expression for NMDA receptor subunits in prefrontal cortex of schizophrenics. J. Neurosci. 16:1996;19-30.
2. Aoki C., Venkatesan C., Go C.-G., Mong J.A., Dawson T.M. Cellular and subcellular localization of NMDA-R1 subunit immunoreactivity in the visual cortex of adult and neonatal rats. J. Neurosci. 14:1994;5202-5222.
3. Audinat E., Lambolez B., Rossier J., Crépel F. Activity-dependent regulation of N-methyl-D-aspartate receptor subunit expression in rat cerebellar granule cells. Eur. J. Neurosci. 6:1994;1792-1800.
4. Blahos J., Wenthold R.J. Relationship between N-methyl-D-aspartate receptor NR1 splice variants and NR2 subunits. J. Biol. Chem. 271:1996;15669-15674.
5. Brose N., Gasic G.P., Vetter D.E., Sullivan J.M., Heinemann S.F. Protein chemical characterisation and immunocytochemical localization of the NMDA receptor subunit NMDA R1. J. Biol. Chem. 268:1993;22663-22671.
6. Buller A., Larson H.C., Schneider B.E., Beaton J.A., Morrisett R.A., Monaghan D.T. The molecular basis of NMDA receptor subtypes: native receptor diversity is predicted by subunit composition. J. Neurosci. 14:1994;5471-5484.
7. Carmignoto G., Vicini S. Activity dependent decrease in NMDA receptor responses during development of the visual cortex. Science. 258:1992;1007-1011.
8. Chazot P.L., Cik M., Stephenson A. Immunological detection of the NMDAR1 glutamate receptor subunit expressed in human embryonic kidney 293 cells and in rat brain. J. Neurochem. 59:1992;1176-1178.
9. Chazot P.L., Coleman S.K., Cik M., Stephenson F.A. Molecular characterization of N-methyl-D-aspartate receptors expressed in mammalian cells yields evidence for the coexistence of three subunit types within a discrete receptor molecule. J. Biol. Chem. 269:1994;24403-24409.
10. Didier M., Xu M., Berman S.A., Bursztajn S. Differential expression and co-assembly of NMDAζ1 and ε subunits in the mouse cerebellum during postnatal development. NeuroReport. 6:1995;2255-2259.
11. Durand G.M., Gregor P., Zheng X., Bennett M.V.L., Uhl G.R., Zukin R.S. Cloning of an apparent splice variant of the rat N-methyl-D-aspartate receptor NMDAR1 with altered sensitivity to polyamines and activators of protein kinase C. Proc. Natl. Acad. Sci. USA. 89:1992;9359-9363.
12. Durand G.M., Bennett M.V.L., Zukin R.S. Splice variants of the N-methyl-D-aspartate receptor NR1 identify domains involved in regulation by polyamines and protein kinase C. Proc. Natl. Acad. Sci. USA. 90:1993;6731-6735.
13. Forrest D., Yuzaki M., Soares H.D., Ng L., Lik D.C., Sheng M., Stewart C.L., Morgan J.I., Connor J.A., Curran T. Targeted disruption of NMDA receptor 1 gene abolishes NMDA response and results in neonatal death. Neuron. 13:1994;325-338.
14. Harveit E., Brandstätter J.H., Sassoè-Pognetto M., Laurie D.J., Seeburg P.H., Wässle H. Localization and developmental expression of the NMDA receptor subunit NR2A in the mammalian retina. J. Comp. Neurol. 348:1994;570-582.
15. Hollmann M., Boulter J., Maron C., Beasley L., Sullivan J., Pecht G., Heinemann S. Zinc potentiates agonist-induced currents at certain splice variants of the NMDA receptor. Neuron. 10:1993;943-954.
16. Honoré T., Drejer J., Nielsen E.Ø., Watkins J.C., Olverman H.J., Nielsen M. Molecular target size analysis of the NMDA-receptor complex in rat cortex. Eur. J. Pharmacol. (Mol. Pharmacol. Sect.). 172:1989;239-247.
17. Ikeda K., Nakasawa M., Mori H., Araki K., Sakimura K., Watanabe M., Inoue Y., Mishina M. Cloning and expression of the ε4 subunit of the NMDA receptor channel. FEBS Lett. 313:1992;34-38.
18. Ishii T., Moriyoshi K., Sugihara H., Sakurada K., Kadotani H., Yokoi M., Akazawa C., Shigemoto R., Mizuno N., Masu M., Nakanishi S. Molecular characterization of the family of the N-methyl-D-aspartate receptor subunits. J. Biol. Chem. 268:1993;2836-2843.
19. Kalb R.G., Lidow M.S., Halsted M.J., Hockfield S. N-Methyl-D-aspartate receptors are transiently expressed in the developing spinal cord ventral horn. Proc. Natl. Acad. Sci. USA. 89:1992;8502-8506.
20. Karp S.J., Masu M., Eki T., Ozawa K., Nakanishi S. Molecular cloning and chromosomal localization of the key subunit of the human N-methyl-D-aspartate receptor. J. Biol. Chem. 268:1993;3728-3733.
21. Köpke A.K.E., Bonk I., Sydow S., Menke H., Spiess J. Characterization of the NR1, NR2A and NR2C receptor proteins. Protein Science. 2:1993;2066-2076.
22. Kornau H.-C., Schenker L.T., Kennedy M.B., Seeburg P.H. Domain interaction between NMDA receptor subunits and the postsynaptic density protein PSD-95. Science. 269:1995;1737-1740.
23. Kutsuwada T., Kashiwabuchi N., Mori H., Sakimura K., Kushiya E., Araki K., Meguro H., Masaki H., Kumanishi T., Arakawa M., Mishina M. Molecular diversity of the NMDA receptor channel. Nature. 358:1992;36-41.
24. Kutsuwada T., Sakimura K., Manabe T., Takayama C., Katakura N., Kushiya E., Natsume R., Watanabe M., Inoue Y., Yagi T., Aizawa S., Arakawa M., Takahashi T., Nakamura Y., Mori H., Mishina M. Impairment of suckling response, trigeminal neuronal pattern formation, and hippocampal LTD in NMDA receptor ε2 subunit mutant mice. Neuron. 16:1994;333-344.
25. D.J. Laurie, I. Bartke, R. Schoepfer, K. Naujoks and P.H. Seeburg, Monoclonal antibodies against the NMDAR2A, -2B and -2C subunits, Eur. J. Neurosci. Suppl. 7 (1994) Abstr. 115.06.
26. Laurie D.J., Seeburg P.H. Ligand affinities at recombinant N-methyl-D-aspartate receptors depend on subunit composition. Eur. J. Pharmacol. 268:1994;335-345.
27. Laurie D.J., Seeburg P.H. Regional and developmental heterogeneity in splicing of the rat brain NMDAR1 mRNA. J. Neurosci. 14:1994;3180-3194.
28. Laurie D.J., Putzke J., Ziegelgänsberger W., Seeburg P.H., Tölle T. The distribution of splice variants of the NMDAR1 subunit mRNA in adult rat brain. Mol. Brain Res. 32:1995;94-108.
29. Le Bourdellès B., Wafford K.A., Kemp J.A., Marshell G., Bain C., Wilcox A.S., Sikela J.M., Whiting P.J. Cloning, functional coexpression, and pharmacological characterisation of human cDNAs encoding NMDA receptor NR1 and NR2A subunits. J. Neurochem. 62:1995;2091-2098.
30. Liu H., Wang H., Sheng M., Jan L.Y., Jan Y.N., Basbaum A.I. Evidence for presynaptic N-methyl-D-asparate autoreceptors in the spinal cord dorsal horn. Proc. Natl. Acad. Sci. USA. 91:1993;8383-8387.
31. J.M. Luque, Z. Bleuel, P. Malherbe and G. Richards, Cellular expression of mRNAs encoding NMDAR1 (splice variants) and NMDAR2 (A-D) receptor subunits in rat CNS, Eur. J. Neurosci., Suppl. 7 (1994) Abst. No. 47.03.
32. 125I-labeled CGP 55802A. Proc. Natl. Acad. Sci. USA. 90:1993;8434-8438.
33. McBain C.J., Mayer M.L. N-Methyl-D-aspartic acid receptor structure and function. Physiol. Rev. 74:1994;723-760.
34. Monyer H., Sprengel R., Schoepfer R., Herb A., Higuchi M., Lomeli H., Burnashev N., Sakmann B., Seeburg P.H. Heteromeric NMDA receptors: molecular and functional distinction of subtypes. Science. 256:1992;1217-1221.
35. Monyer H., Burnashev N., Laurie D.J., Sakmann B., Seeburg P.H. Developmental and regional expression in the rat brain and functional properties of four NMDA receptors. Neuron. 12:1994;529-540.
36. Moriyoshi K., Masu M., Ishii T., Shigemoto R., Mizuno N., Nakanishi S. Molecular cloning and characterization of the rat NMDA receptor. Nature. 354:1991;31-37.
37. Niethammer M., Kim E., Sheng M. Interaction between the C-terminus of NMDA receptor subunits and multiple members of the PSD-95 family of membrane-associated guanylate cyclases. J. Neurosci. 16:1996;2157-2163.
38. Okakura K., Yamatodani A., Mochizuki T., Horii A., Wada H. Glutamatergic regulation of histamine release from rat hypothalamus. Eur. J. Pharmacol. 213:1992;189-192.
39. Petralia R.S., Yokotani N., Wenthold R.J. Light and electron microscope distribution of the NMDA receptor subunit NMDAR1 in the rat nervous system using a selective anti-peptide antibody. J. Neurosci. 14:1994;667-696.
40. Petralia R.S., Wang Y.X., Wenthold R.J. The NMDA receptor subunits NR2A and NR2B show histological and ultrastructural localization patterns similar to those of NR1. J. Neurosci. 14:1994;6102-6120.
41. Planells-Cases R., Sun W., Ferrer-Montiel A.V., Montal M. Molecular cloning, functional expression, and pharmacological characterization of an N-methyl-D-aspartate receptor subunit from human brain. Proc. Natl. Acad. Sci. USA. 90:1993;5057-5061.
42. Portera-Cailleau C., Price D.L., Martin L.J. N-Methyl-D-aspartate receptor proteins NR2A and NR2B are differentially distributed in the developing rat central nervous system as revealed by subunit-specific antibodies. J. Neurochem. 66:1996;692-700.
43. Riva M.A., Tascedda F., Molteni R., Racagni G. Regulation of NMDA receptor subunit mRNA expression in the rat brain during postnatal development. Mol. Brain Res. 25:1994;209-216.
44. Sakimura K., Kutsuwada T., Ito I., Manabe T., Takayama C., Kushiya E., Yaga T., Aizawa S., Inoue Y., Sugiyama H., Mishina M. Reduced hippocampal LTP and spatial learning in mice lacking NMDA receptor ε1 subunit. Nature. 373:1995;151-155.
45. Schoepfer R. The pRSET family of T7 promotor expression vectors for Escherichia coli. Gene. 124:1993;83-85.
46. Sheng M., Cummings J., Roldan L.A., Jan Y.N., Jan L.Y. Changing subunit composition of heteromeric NMDA receptors during development of rat cortex. Nature. 368:1994;144-147.
47. Standaert D.G., Testa C.M., Penney J.B., Young A.B. Alternatively spliced isoforms of the NMDAR1 glutamate receptor subunit: differential expression in the basal ganglia of the rat. Neurosci. Lett. 152:1993;161-164.
48. Sucher N.J., Brose N., Deitcher D.L., Awobuluyi M., Gasic G.P., Bading H., Cepko C.L., Greenberg M.E., Jahn R., Heinemann S.F., Lipton S.A. Expression of endogenous NMDAR1 transcripts without receptor protein suggests post-transcriptional control in PC12 cells. J. Biol. Chem. 268:1993;22299-22304.
49. Sugihara H., Moriyoshi K., Ishii T., Masu M., Nakanishi S. Structures and properties of seven isoforms of the NMDA receptor generated by alternative splicing. Biochem. Biophys. Res. Commun. 185:1992;826-832.
50. Tingley W.G., Roche K.W., Thompson A.K., Huganir R.L. Regulation of NMDA receptor phosphorylation by alternative splicing of the C-terminal domain. Nature. 364:1993;70-73.
51. Tölle T., Berthele A., Ziegelgänsberger W., Seeburg P.H., Wisden W. The differential expression of 16 NMDA and non-NMDA receptor subunits in the rat spinal cord and in periaqueductal gray. J. Neurosci. 13:1993;5009-5028.
52. Wafford K., Bain C.J., Le Bourdelles B., Whiting P.J., Kemp J.A. Preferential coassembly of recombinant NMDA receptors composed of three different subunits. NeuroReport. 4:1993;1347-1349.
53. Wang Y., Bosy T.Z., Yasuda R.P., Grayson D.R., Vicini S., Pizzorusso T., Wolfe B. Characterization of NMDA receptor subunit-specific antibodies: distribution of NR2A and NR2B receptor subunits in rat brain and ontogenic profile in the cerebellum. J. Neurochem. 65:1995;176-183.
54. Wenzel A., Scheurer L., Künzi R., Fritschy J.M., Mohler H., Benke D. Distribution of NMDA receptor subunit proteins NR2A, -2B, -2C and -2D in rat brain. NeuroReport. 7:1995;45-48.
55. Wenzel A., Villa M., Mohler H., Benke D. Developmental and regional expression of the NMDA receptor subtypes containing the NR2D subunit in rat brain. J. Neurochem. 66:1996;1240-1248.
56. Yamazaki M., Mori H., Araki K., Mori K.J., Mishina M. Cloning, expression and modulation of a mouse NMDA receptor subunit. FEBS Lett. 300:1992;39-45.
57. Zhong J., Carrozza D.P., Williams K., Pritchett D.B., Molinoff P.B. Expression of mRNAs encoding subunits of the NMDA receptor in developing rat brain. J. Neurochem. 64:1995;531-539.