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Volumn 71, Issue 11, 1997, Pages 8321-8329

Readthrough activation of early adenovirus E1b gene transcription

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[No Author keywords available]

Indexed keywords

VIRUS PROTEIN;

EID: 0030864074     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/jvi.71.11.8321-8329.1997     Document Type: Article
Times cited : (17)

References (57)
  • 1
    • 0021718180 scopus 로고
    • Effect on transformation of mutations in the early region 1b-encoded 21- And 55-kilodalton proteins of adenovirus 5
    • Babiss, L. E., P. B. Fisher, and H. S. Ginsberg. 1984. Effect on transformation of mutations in the early region 1b-encoded 21-and 55-kilodalton proteins of adenovirus 5. J. Virol. 52:389-395.
    • (1984) J. Virol. , vol.52 , pp. 389-395
    • Babiss, L.E.1    Fisher, P.B.2    Ginsberg, H.S.3
  • 2
    • 0018581935 scopus 로고
    • Pre-early adenovirus 5 gene product regulates synthesis of early viral messenger RNAs
    • Berk, A. J., F. Lee, T. Harrison, J. Williams, and P. A. Sharp. 1979. Pre-early adenovirus 5 gene product regulates synthesis of early viral messenger RNAs. Cell 17:935-944.
    • (1979) Cell , vol.17 , pp. 935-944
    • Berk, A.J.1    Lee, F.2    Harrison, T.3    Williams, J.4    Sharp, P.A.5
  • 3
    • 0018232933 scopus 로고
    • Structure of the adenovirus 2 early mRNAs
    • Berk, A. J., and P. A. Sharp. 1978. Structure of the adenovirus 2 early mRNAs. Cell 14:695-711.
    • (1978) Cell , vol.14 , pp. 695-711
    • Berk, A.J.1    Sharp, P.A.2
  • 4
    • 0028879804 scopus 로고
    • DNA sequence of the deletion/insertion in early region 3 of Ad5 dl309
    • Bett, A. J., V. Krougliak, and F. L. Graham. 1995. DNA sequence of the deletion/insertion in early region 3 of Ad5 dl309. Virus Res. 39:75-82.
    • (1995) Virus Res. , vol.39 , pp. 75-82
    • Bett, A.J.1    Krougliak, V.2    Graham, F.L.3
  • 5
    • 0029802124 scopus 로고    scopus 로고
    • Radical mutations reveal TATA-box binding protein surfaces required for activated transcription in vivo
    • Bryant, G. O., L. S. Martel, S. K. Burley, and A. J. Berk. 1996. Radical mutations reveal TATA-box binding protein surfaces required for activated transcription in vivo. Genes Dev. 10:2491-2504.
    • (1996) Genes Dev. , vol.10 , pp. 2491-2504
    • Bryant, G.O.1    Martel, L.S.2    Burley, S.K.3    Berk, A.J.4
  • 6
    • 0017262753 scopus 로고
    • Viral transcription in KB cells infected by temperature-sensitive "early" mutants of adenovirus type 5
    • Carter, T. H., and H. S. Ginsberg. 1976. Viral transcription in KB cells infected by temperature-sensitive "early" mutants of adenovirus type 5. J. Virol. 18:156-166.
    • (1976) J. Virol. , vol.18 , pp. 156-166
    • Carter, T.H.1    Ginsberg, H.S.2
  • 8
    • 0027251720 scopus 로고
    • Wild-type p53 mediates apoptosis by E1A, which is inhibited by E1B
    • Debbas, M., and E. White. 1993. Wild-type p53 mediates apoptosis by E1A, which is inhibited by E1B. Genes Dev. 7:546-554.
    • (1993) Genes Dev. , vol.7 , pp. 546-554
    • Debbas, M.1    White, E.2
  • 9
    • 0027215176 scopus 로고
    • 3′ RNA processing efficiency plays a primary role in generating termination-competent RNA polymerase II elongation complexes
    • Edwalds-Gilbert, G., J. Prescott, and E. Falck-Pedersen. 1993. 3′ RNA processing efficiency plays a primary role in generating termination-competent RNA polymerase II elongation complexes. Mol. Cell. Biol. 13:3472-3480.
    • (1993) Mol. Cell. Biol. , vol.13 , pp. 3472-3480
    • Edwalds-Gilbert, G.1    Prescott, J.2    Falck-Pedersen, E.3
  • 10
    • 0022416630 scopus 로고
    • Transcription termination within the E1a gene of adenovirus induced by insertion of the mouse β-major globin terminator element
    • Falck-Pedersen, E., J. Logan, T. Shenk, and J. E. Darnell, Jr. 1985. Transcription termination within the E1a gene of adenovirus induced by insertion of the mouse β-major globin terminator element. Cell 40:897-905.
    • (1985) Cell , vol.40 , pp. 897-905
    • Falck-Pedersen, E.1    Logan, J.2    Shenk, T.3    Darnell Jr., J.E.4
  • 11
    • 0018582958 scopus 로고
    • Location of additional early gene sequences in the adenoviral chromosome
    • Galos, R. S., J. Williams, M. Binger, and S. J. Flint. 1979. Location of additional early gene sequences in the adenoviral chromosome. Cell 17:945-956.
    • (1979) Cell , vol.17 , pp. 945-956
    • Galos, R.S.1    Williams, J.2    Binger, M.3    Flint, S.J.4
  • 12
    • 0028221002 scopus 로고
    • The zinc finger region of the adenovirus E1a transactivating domain complexes with the TATA box binding protein
    • Geisberg, J. V., W. S. Lee, A. J. Berk, and R. P. Ricciardi. 1994. The zinc finger region of the adenovirus E1a transactivating domain complexes with the TATA box binding protein. Proc. Natl. Acad. Sci. USA 91:2488-2492.
    • (1994) Proc. Natl. Acad. Sci. USA , vol.91 , pp. 2488-2492
    • Geisberg, J.V.1    Lee, W.S.2    Berk, A.J.3    Ricciardi, R.P.4
  • 14
    • 0017715076 scopus 로고
    • Adenovirus DNA-binding protein in cells infected with wild-type 5 adenovirus and two DNA-minus, temperature-sensitive mutants, H5ts125 and H5ts149
    • Ginsberg, H. S., U. Lundholm, and T. Linné. 1977. Adenovirus DNA-binding protein in cells infected with wild-type 5 adenovirus and two DNA-minus, temperature-sensitive mutants, H5ts125 and H5ts149. J. Virol. 23:142-151.
    • (1977) J. Virol. , vol.23 , pp. 142-151
    • Ginsberg, H.S.1    Lundholm, U.2    Linné, T.3
  • 15
    • 0017710978 scopus 로고
    • Characteristics of a human cell line transformed by DNA from human adenovirus type 5
    • Graham, F. L., J. Smiley, W. C. Russell, and R. Nairn. 1977. Characteristics of a human cell line transformed by DNA from human adenovirus type 5. J. Gen. Virol. 36:59-72.
    • (1977) J. Gen. Virol. , vol.36 , pp. 59-72
    • Graham, F.L.1    Smiley, J.2    Russell, W.C.3    Nairn, R.4
  • 16
    • 0020791518 scopus 로고
    • The adenovirus type 5 E1a transcriptional control region contains a duplicated enhancer element
    • Hearing, P., and T. Shenk. 1983. The adenovirus type 5 E1A transcriptional control region contains a duplicated enhancer element. Cell 33:695-703.
    • (1983) Cell , vol.33 , pp. 695-703
    • Hearing, P.1    Shenk, T.2
  • 17
    • 0021104422 scopus 로고
    • Functional analysis of the nucleotide sequence surrounding the cap site for adenovirus type 5 region E1a messenger RNAs
    • Hearing, P., and T. Shenk. 1983. Functional analysis of the nucleotide sequence surrounding the cap site for adenovirus type 5 region E1a messenger RNAs. J. Mol. Biol. 167:809-822.
    • (1983) J. Mol. Biol. , vol.167 , pp. 809-822
    • Hearing, P.1    Shenk, T.2
  • 18
    • 0022197249 scopus 로고
    • Sequence-independent autoregulation of the adenovirus type 5 E1A transcription unit
    • Hearing, P., and T. Shenk. 1985. Sequence-independent autoregulation of the adenovirus type 5 E1A transcription unit. Mol. Cell. Biol. 5:3214-3221.
    • (1985) Mol. Cell. Biol. , vol.5 , pp. 3214-3221
    • Hearing, P.1    Shenk, T.2
  • 19
    • 0024325630 scopus 로고
    • Enhancement of bacteriophage T4 late transcription by components of the T4 DNA replication apparatus
    • Herendeen, D. R., G. A. Kassavetis, J. Barry, B. M. Alberts, and E. P. Geiduschek. 1989. Enhancement of bacteriophage T4 late transcription by components of the T4 DNA replication apparatus. Science 245:952-958.
    • (1989) Science , vol.245 , pp. 952-958
    • Herendeen, D.R.1    Kassavetis, G.A.2    Barry, J.3    Alberts, B.M.4    Geiduschek, E.P.5
  • 20
    • 0026752261 scopus 로고
    • A transcriptional enhancer whose function imposes a requirement that proteins track along DNA
    • Herendeen, D. R., G. A. Kassavetis, and E. P. Geiduschek. 1992. A transcriptional enhancer whose function imposes a requirement that proteins track along DNA. Science 256:1298-1303.
    • (1992) Science , vol.256 , pp. 1298-1303
    • Herendeen, D.R.1    Kassavetis, G.A.2    Geiduschek, E.P.3
  • 21
    • 0025291710 scopus 로고
    • An RNA polymerase-binding protein that is required for communication between an enhancer and a promoter
    • Herendeen, D. R., K. P. Williams, G. A. Kassavetis, and E. P. Geiduschek. 1990. An RNA polymerase-binding protein that is required for communication between an enhancer and a promoter. Science 248:573-578.
    • (1990) Science , vol.248 , pp. 573-578
    • Herendeen, D.R.1    Williams, K.P.2    Kassavetis, G.A.3    Geiduschek, E.P.4
  • 22
    • 0005889285 scopus 로고
    • Control of transcription from the r strand of bacteriophage lambda
    • Herskowitz, I., and E. Singer. 1970. Control of transcription from the r strand of bacteriophage lambda. Cold Spring Harbor Symp. Quant. Biol. 35:355-368.
    • (1970) Cold Spring Harbor Symp. Quant. Biol. , vol.35 , pp. 355-368
    • Herskowitz, I.1    Singer, E.2
  • 23
    • 0023732753 scopus 로고
    • Mechanism of action of a yeast activator: Direct effect of GAL4 derivatives on mammalian TFIID-promoter interactions
    • Horikoshi, M., M. F. Carey, H. Kakidani, and R. G. Roeder. 1988. Mechanism of action of a yeast activator: direct effect of GAL4 derivatives on mammalian TFIID-promoter interactions. Cell 54:665-669.
    • (1988) Cell , vol.54 , pp. 665-669
    • Horikoshi, M.1    Carey, M.F.2    Kakidani, H.3    Roeder, R.G.4
  • 24
    • 0018650016 scopus 로고
    • An adenovirus type 5 early gene function regulates expression of other early viral genes
    • Jones, N., and T. Shenk. 1979. An adenovirus type 5 early gene function regulates expression of other early viral genes. Proc. Natl. Acad. Sci. USA 76:3665-3669.
    • (1979) Proc. Natl. Acad. Sci. USA , vol.76 , pp. 3665-3669
    • Jones, N.1    Shenk, T.2
  • 25
    • 0018581742 scopus 로고
    • Isolation of adenovirus type 5 host range deletion mutants defective for transformation of rat embryo cells
    • Jones, N., and T. Shenk. 1979. Isolation of adenovirus type 5 host range deletion mutants defective for transformation of rat embryo cells. Cell 17: 683-689.
    • (1979) Cell , vol.17 , pp. 683-689
    • Jones, N.1    Shenk, T.2
  • 26
    • 0018896508 scopus 로고
    • The structure of adenovirus 2 early nuclear and cytoplasmic RNAs
    • Kitchingman, G. R., and H. Westphal. 1980. The structure of adenovirus 2 early nuclear and cytoplasmic RNAs. J Mol. Biol. 137:23-48.
    • (1980) J Mol. Biol. , vol.137 , pp. 23-48
    • Kitchingman, G.R.1    Westphal, H.2
  • 27
    • 0026040678 scopus 로고
    • Adenovirus E1a activation domain binds the basic repeat in the TATA box transcription factor
    • Lee, W. S., C. Kao, G. O. Bryant, X. Liu, and A. J. Berk. 1991 Adenovirus E1a activation domain binds the basic repeat in the TATA box transcription factor. Cell 67:365-376.
    • (1991) Cell , vol.67 , pp. 365-376
    • Lee, W.S.1    Kao, C.2    Bryant, G.O.3    Liu, X.4    Berk, A.J.5
  • 30
    • 0020591334 scopus 로고
    • Inhibition of RNA cleavage but not polyadenylation by a point mutation in mRNA 3′ consensus sequence AAUAAA
    • Montell, C., E. F. Fisher, M. H. Caruthers, and A. J. Berk. 1983. Inhibition of RNA cleavage but not polyadenylation by a point mutation in mRNA 3′ consensus sequence AAUAAA. Nature 305:600-605.
    • (1983) Nature , vol.305 , pp. 600-605
    • Montell, C.1    Fisher, E.F.2    Caruthers, M.H.3    Berk, A.J.4
  • 31
  • 32
    • 0023930179 scopus 로고
    • Organization of the transcriptional control region of the E1b gene of adenovirus type 5
    • Parks, C. L., S. Banerjee, and D. J. Spector. 1988. Organization of the transcriptional control region of the E1b gene of adenovirus type 5. J. Virol. 62:54-67.
    • (1988) J. Virol. , vol.62 , pp. 54-67
    • Parks, C.L.1    Banerjee, S.2    Spector, D.J.3
  • 33
    • 0025293144 scopus 로고
    • cis-Dominant defect in activation of adenovirus type 5 E1b early RNA synthesis
    • Parks, C. L., and D. J. Spector. 1990. cis-Dominant defect in activation of adenovirus type 5 E1b early RNA synthesis. J. Virol. 64:2780-2787.
    • (1990) J. Virol. , vol.64 , pp. 2780-2787
    • Parks, C.L.1    Spector, D.J.2
  • 34
    • 0017284089 scopus 로고
    • Hybridization maps of early and late messenger RNA sequences on the adenovirus type 2 genome
    • Pettersson, U., C. Tibbetts, and L. Philipson. 1976. Hybridization maps of early and late messenger RNA sequences on the adenovirus type 2 genome. J. Mol. Biol. 101:479-501.
    • (1976) J. Mol. Biol. , vol.101 , pp. 479-501
    • Pettersson, U.1    Tibbetts, C.2    Philipson, L.3
  • 36
    • 0026639932 scopus 로고
    • The adenovirus E1A proteins induce apoptosis, which is inhibited by the E1B 19-kDa and Bcl-2 proteins
    • Rao, L., M. Debbas, P. Sabbatini, D. Hockenbery, S. Korsemeyer, and E. White. 1992. The adenovirus E1A proteins induce apoptosis, which is inhibited by the E1B 19-kDa and Bcl-2 proteins. Proc. Natl. Acad. Sci. USA 89:7742-7746.
    • (1992) Proc. Natl. Acad. Sci. USA , vol.89 , pp. 7742-7746
    • Rao, L.1    Debbas, M.2    Sabbatini, P.3    Hockenbery, D.4    Korsemeyer, S.5    White, E.6
  • 38
    • 0023078285 scopus 로고
    • Identification of regulatory elements of cloned genes with functional assays
    • Rosenthal, N. 1987. Identification of regulatory elements of cloned genes with functional assays. Methods Enzymol. 152:704-721.
    • (1987) Methods Enzymol. , vol.152 , pp. 704-721
    • Rosenthal, N.1
  • 39
    • 0027981341 scopus 로고
    • Use of a macromolecular crowding agent to dissect interactions and define functions in transcriptional activation by a DNA-tracking protein: Bacteriophage T4 gene 45 protein and late transcription
    • Sanders, G. M., G. A. Kassavetis, and E. P. Geiduschek. 1994. Use of a macromolecular crowding agent to dissect interactions and define functions in transcriptional activation by a DNA-tracking protein: bacteriophage T4 gene 45 protein and late transcription. Proc. Natl. Acad. Sci. USA 91:7703-7704.
    • (1994) Proc. Natl. Acad. Sci. USA , vol.91 , pp. 7703-7704
    • Sanders, G.M.1    Kassavetis, G.A.2    Geiduschek, E.P.3
  • 40
    • 0024206796 scopus 로고
    • Multiple transcription terminators in E1a and E1b genes of adenovirus type 5
    • Sato, K., K. Kobayashi, and K. Hosokawa. 1988. Multiple transcription terminators in E1a and E1b genes of adenovirus type 5. Gene 71:371-379.
    • (1988) Gene , vol.71 , pp. 371-379
    • Sato, K.1    Kobayashi, K.2    Hosokawa, K.3
  • 41
    • 0025314719 scopus 로고
    • Adenovirus terminal protein mediates both nuclear matrix association and efficient transcription of adenovirus DNA
    • Schaack, J., W. Y. Ho, P. Freimuth, and T. Shenk. 1990. Adenovirus terminal protein mediates both nuclear matrix association and efficient transcription of adenovirus DNA. Genes Dev. 4:1197-1208.
    • (1990) Genes Dev. , vol.4 , pp. 1197-1208
    • Schaack, J.1    Ho, W.Y.2    Freimuth, P.3    Shenk, T.4
  • 42
    • 0024390631 scopus 로고
    • Sp1 activates transcription without enhancing DNA-binding activity of the TATA box factor
    • Schmidt, M. C., Q. Zhou, and A. J. Berk. 1989. Sp1 activates transcription without enhancing DNA-binding activity of the TATA box factor. Mol. Cell. Biol. 9:3299-3307.
    • (1989) Mol. Cell. Biol. , vol.9 , pp. 3299-3307
    • Schmidt, M.C.1    Zhou, Q.2    Berk, A.J.3
  • 43
    • 0023687042 scopus 로고
    • A simple phase-extraction assay for chloramphenicol acyltransferase activity
    • Seed, B., and J.-Y. Sheen. 1988. A simple phase-extraction assay for chloramphenicol acyltransferase activity. Gene 67:271-277.
    • (1988) Gene , vol.67 , pp. 271-277
    • Seed, B.1    Sheen, J.-Y.2
  • 44
    • 0016306680 scopus 로고
    • Mapping of adenovirus 2 RNA sequences in lytically infected cells and transformed cell lines
    • Sharp, P. A., P. H. Gallimore, and S. J. Flint. 1974. Mapping of adenovirus 2 RNA sequences in lytically infected cells and transformed cell lines. Cold Spring Harbor Symp. Quant. Biol. 39:457-474.
    • (1974) Cold Spring Harbor Symp. Quant. Biol. , vol.39 , pp. 457-474
    • Sharp, P.A.1    Gallimore, P.H.2    Flint, S.J.3
  • 45
    • 1842345480 scopus 로고    scopus 로고
    • Unpublished data
    • Spector, D. J. Unpublished data.
    • Spector, D.J.1
  • 46
    • 0027328601 scopus 로고
    • A multicomponent cis-activator of transcription of the E1b gene of adenovirus type 5
    • Spector, D. J., C. L. Parks, and R. A. Knittle. 1993. A multicomponent cis-activator of transcription of the E1b gene of adenovirus type 5. Virology 194:128-136.
    • (1993) Virology , vol.194 , pp. 128-136
    • Spector, D.J.1    Parks, C.L.2    Knittle, R.A.3
  • 47
    • 0002326121 scopus 로고
    • Construction and isolation of recombinant adenoviruses with gene replacements
    • Spector, D. J., and L. A. Samaniego. 1995. Construction and isolation of recombinant adenoviruses with gene replacements. Methods Mol. Genet. 7:31-44.
    • (1995) Methods Mol. Genet. , vol.7 , pp. 31-44
    • Spector, D.J.1    Samaniego, L.A.2
  • 48
    • 0020381602 scopus 로고
    • Purification of an adenovirus-coded DNA polymerase that is required for initiation of DNA replication
    • Stillman, B. W., F. Tamanoi, and M. B. Mathews. 1982. Purification of an adenovirus-coded DNA polymerase that is required for initiation of DNA replication. Cell 31:613-623.
    • (1982) Cell , vol.31 , pp. 613-623
    • Stillman, B.W.1    Tamanoi, F.2    Mathews, M.B.3
  • 49
    • 0027464264 scopus 로고
    • maj glohin transcription termination region: A spacing sequence is required between the poly(A) signal sequence and multiple downstream termination elements
    • maj glohin transcription termination region: a spacing sequence is required between the poly(A) signal sequence and multiple downstream termination elements. Mol. Cell. Biol. 13:578-587.
    • (1993) Mol. Cell. Biol. , vol.13 , pp. 578-587
    • Tantravahi, J.1    Alvira, M.2    Falck-Pedersen, E.3
  • 50
    • 0019215848 scopus 로고
    • DNA replication and the early to late transition in adenovirus infection
    • Thomas, G. P., and M. B. Mathews. 1980. DNA replication and the early to late transition in adenovirus infection. Cell 22:523-533.
    • (1980) Cell , vol.22 , pp. 523-533
    • Thomas, G.P.1    Mathews, M.B.2
  • 51
    • 0028350651 scopus 로고
    • Transcriptional activation by a DNA-tracking protein: Structural consequences of enhancement at the T4 late promoter
    • Tinker, R. L., K. P. Williams, G. A. Kassavetis, and E. P. Geiduschek. 1994. Transcriptional activation by a DNA-tracking protein: structural consequences of enhancement at the T4 late promoter. Cell 77:225-237.
    • (1994) Cell , vol.77 , pp. 225-237
    • Tinker, R.L.1    Williams, K.P.2    Kassavetis, G.A.3    Geiduschek, E.P.4
  • 52
    • 0020694133 scopus 로고
    • The genetic analysis of recombination using adenovirus overlapping terminal DNA fragments
    • Volkert, F. C., and C. S. H. Young. 1983. The genetic analysis of recombination using adenovirus overlapping terminal DNA fragments. Virology 125:175-193.
    • (1983) Virology , vol.125 , pp. 175-193
    • Volkert, F.C.1    Young, C.S.H.2
  • 53
    • 0023925848 scopus 로고
    • Two regions of the adenovirus early region 1A proteins are required for transformation
    • Whyte, P., H. E. Ruley, and E. Harlow. 1988. Two regions of the adenovirus early region 1A proteins are required for transformation. J. Virol. 62:257-265.
    • (1988) J. Virol. , vol.62 , pp. 257-265
    • Whyte, P.1    Ruley, H.E.2    Harlow, E.3
  • 54
    • 0024519708 scopus 로고
    • Linear adenovirus DNA is organized into supercoiled domains in virus particles
    • Wong, M.-L., and M.-T. Hsu. 1989. Linear adenovirus DNA is organized into supercoiled domains in virus particles. Nucleic Acids Res. 17:3535-3550.
    • (1989) Nucleic Acids Res. , vol.17 , pp. 3535-3550
    • Wong, M.-L.1    Hsu, M.-T.2
  • 55
    • 0023661185 scopus 로고
    • Binding of transcription factor TFIID to the major late promoter during in vitro nucleosome assembly potentiates subsequent initiation by RNA polymerase II
    • Workman, J. L., and R. G. Roeder. 1987. Binding of transcription factor TFIID to the major late promoter during in vitro nucleosome assembly potentiates subsequent initiation by RNA polymerase II. Cell 51:613-622.
    • (1987) Cell , vol.51 , pp. 613-622
    • Workman, J.L.1    Roeder, R.G.2
  • 56
    • 0023806401 scopus 로고
    • Transcriptional activation by the pseudorabies virus immediate early protein requires the TATA box element in the adenovirus 2 E1b promoter
    • Wu, L., and A. J. Berk. 1988. Transcriptional activation by the pseudorabies virus immediate early protein requires the TATA box element in the adenovirus 2 E1b promoter. Virology 167:318-322.
    • (1988) Virology , vol.167 , pp. 318-322
    • Wu, L.1    Berk, A.J.2
  • 57
    • 0023120984 scopus 로고
    • A TATA box implicated in E1a transcriptional activation of a simple adenovirus 2 promoter
    • Wu, L., D. S. E. Rosser, M. C. Schmidt, and A. Berk. 1987. A TATA box implicated in E1a transcriptional activation of a simple adenovirus 2 promoter. Nature 326:512-515.
    • (1987) Nature , vol.326 , pp. 512-515
    • Wu, L.1    Rosser, D.S.E.2    Schmidt, M.C.3    Berk, A.4


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