The N-terminal side of the origin-binding domain of simian virus 40 large T antigen is involved in A/T untwisting

Journal of Virology

Volumn 71, Issue 11, 1997, Pages 8743-8749

  Chen, Lei ^a   Joo, Woo S ^b   Bullock, Peter A ^b   Simmons, Daniel T ^a  

^a UNIVERSITY OF DELAWARE   (United States)

^b TUFTS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

VIRUS DNA; VIRUS LARGE T ANTIGEN; VIRUS PROTEIN;

AMINO ACID SUBSTITUTION; ARTICLE; DNA BINDING; DNA REPLICATION; NONHUMAN; PRIORITY JOURNAL; SIMIAN VIRUS 40; SITE DIRECTED MUTAGENESIS; VIRUS MUTANT; VIRUS REPLICATION;

EID: 0030863406     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/jvi.71.11.8743-8749.1997     Document Type: Article

References (70)

1. Arthur, A. K., A. Höss, and E. Fanning. 1988. Expression of simian virus 40 T antigen in Escherichia coli: localization of T-antigen origin DNA-binding domain to within 129 amino acids. J. Virol. 62:1999-2006.
2. Borowiec, J., and J. Hurwitz. 1988. Localized melting and structural changes in the SV40 origin of replication induced by T-antigen. EMBO J. 7:3149-3158.
3. Borowiec, J. A. 1992. Inhibition of structural changes in the simian virus 40 core origin of replication by mutations of essential origin sequences. J. Virol. 66:5248-5255.
4. Borowiec, J. A., F. B. Dean, and J. Hurwitz. 1991. Differential induction of structural changes in the simian virus 40 origin of replication by T antigen. J. Virol. 65:1228-1235.
5. Bullock, P. A., W. S. Joo, K. R. Sreekumar, and C. Mello. 1997. Initiation of SV40 DNA replication in vitro: analysis of the role played by sequences flanking the core origin on initial synthesis events. Virology 227:460-473.
6. Bullock, P. A., Y. S. Seo, and J. Hurwitz. 1991. Initiation of simian virus 40 DNA synthesis in vitro. Mol. Cell. Biol. 11:2350-2361.
7. Cegielska, A., I. Moarefi, E. Fanning, and D. M. Virshup. 1994. T-antigen kinase inhibits simian virus 40 DNA replication by phosphorylation of intact T antigen on serines 120 and 123. J. Virol. 68:269-275.
8. Collins, K. L., and T. J. Kelly. 1991. Effects of T antigen and replication protein A on the initiation of DNA synthesis by DNA polymerase α-primase. Mol. Cell. Biol. 11:2108-2115.
9. Dean, F. B., Y. Borowiec, Y. Ishimi, S. Deb, P. Tegtmeyer, and J. Hurwitz. 1987. Simian virus 40 large tumor antigen requires three core replication origin domains for DNA unwinding and replication in vitro. Proc. Natl. Acad. Sci. USA 84:8267-8271.
10. Dean, F. B., P. Bullock, Y. Murakami, C. R. Wobbe, L. Weissbach, and J. Hurwitz. 1987. Simian virus 40 (SV40) DNA replication: SV40 large T antigen unwinds DNA containing the SV40 origin of replication. Proc. Natl. Acad. Sci. USA 84:16-20.
11. Dean, F. B., and J. Hurwitz. 1991. Simian virus 40 large T antigen untwists DNA at the origin of DNA replication. J. Biol. Chem. 266:5062-5071.
12. Deb, S., A. L. DeLucia, C. P. Baur, A. Koff, and P. Tegtmeyer. 1986. Domain structure of simian virus 40 core origin of replication. Mol. Cell. Biol. 6: 1663-1670.
13. Deb, S., A. L. DeLucia, A. Koff, S. Tsui, and P. Tegtmeyer. 1986. The adenine-thymine domain of the simian virus 40 core origin directs DNA bending and coordinately regulates DNA replication. Mol. Cell. Biol. 6: 4578-4584.
14. Deb, S., S. Tsui, A. Koff, A. L. DeLucia, R. Parsons, and P. Tegtmeyer. 1987. The T-antigen hinding domain of the simian virus 40 core orisin of replication. J. Virol. 61:2143-2149.
15. DeLucia, A. L., S. Deb, K. Partin, and P. Tegtmeyer. 1986. Functional interactions of simian virus 40 core origin of replication with flanking regulatory sequences. J. Virol. 57:138-144.
16. Denis, D., and P. A. Bullock. 1993. Primer-DNA formation during simian virus 40 DNA replication in vitro. Mol. Cell. Biol. 13:2882-2890.
17. Dodson, M., F. B. Dean, P. Bullock, H. Echols, and J. Hurwitz. 1987. Unwinding of duplex DNA from the SV40 origin of replication by T antigen. Science 238:964-967.
18. Dornreiter, I., W. C. Copeland, and T. S.-F. Wang. 1993. Initiation of simian virus 40 DNA replication requires the interaction of a specific domain of human DNA polymerase a with large T antigen. Mol. Cell. Biol. 13:809-820.
19. Dornreiter, I., L. F. Erdile, I. U. Gilbert, D. von Winkler, T. J. Kelly, and E. Fanning. 1992. Interaction of DNA polymerase alpha-primase with cellular replication protein A and SV40 T antigen. EMBO J. 11:769-776.
20. Dornreiter, I., A. Hoss, A. K. Arthur, and E. Fanning. 1990. SV40 T antigen binds directly to the catalytic subunit of DNA polymerase α. EMBO J. 9:3329-3336.
21. Eki, T., T. Matsumoto, Y. Murakami, and J. Hurwitz. 1992. The replication of DNA containing the simian virus 40 origin by the monopolymerase and dipolymerase systems. J. Biol. Chem. 267:7284-7294.
22. Goetz, G. S., F. B. Dean, J. Hurwitz, and S. W. Matson. 1988. The unwinding of duplex regions in DNA by the simian virus 40 large tumor antigen-associated DNA helicase activity. J. Biol. Chem. 263:383-392.
23. Gralla, J. D. 1985. Rapid "footprinting" on supercoiled DNA. Proc. Natl. Acad. Sci. USA 82:3078-3081.
24. Grasser, F. A., K. Mann, and G. Walter. 1987. Removal of serine phosphates from simian virus 40 large T antigen increases its ability to stimulate DNA replication in vitro but has no effect on ATPase and DNA binding. J. Virol. 61:3373-3380.
25. Gurney, E. G., R. O. Harrison, and J. Fenno. 1980. Monoclonal antibodies against simian virus 40 T antigens: evidence for distinct subclasses of large T antigen and for similarities among nonviral T antigens. J. Virol. 34:752-763.
26. Gurney, E. G., S. Tamowski, and W. Deppert. 1986. Antigenic binding sites of monoclonal antibodies specific for simian virus 40 large T antigen. J. Virol. 57:1168-1172.
27. Han, F. X., and L. H. Hurley. 1996. A model for the T-antigen-induced structural alteration of the SV40 replication origin based upon experiments with specific probes for bent, straight, and unwound DNA. Biochemistry 35:7993-8001.
28. Harlow, E., L. V. Crawford, D. C. Pim, and N. M. Williamson. 1981. Monoclonal antibodies specific for simian virus 40 tumor antigen. J. Virol. 39:861-869.
29. Kalderon, D., and A. E. Smith. 1984. In vitro mutagenesis of a putative DNA binding domain of SV40 large-T. Virology 139:109-137.
30. Kenny, M. K., S. H. Lee, and J. Hurwitz. 1989. Multiple functions of human single-stranded-DNA binding protein in simian virus 40 DNA replication: single-strand stabilization and stimulation of DNA polymerase α and δ. Proc. Natl. Acad. Sci. USA 86:9757-9761.
31. Lin, H.-J. L., R. Upson, and D. T. Simmons. 1992. Nonspecific DNA binding activity of simian virus 40 large T antigen: evidence for the cooperation of two regions for full activity. J. Virol. 66:5443-5452.
32. Loeber, G., R. Parsons, and P. Tegtmeyer. 1989. The zinc finger region of simian virus 40 large T antigen. J. Virol. 63:94-100.
33. Luo, X., D. G. Sanford, P. A. Bullock, and W. W. Bachovchin. 1996. Solution structure of the origin DNA-binding domain of SV40 T-antigen. Nat. Struct. Biol. 3:1034-1039.
34. Mastrangelo, I. A., P. V. C. Hough, J. S. Wall, M. Dodson, F. B. Dean, and J. Hurwitz. 1989. ATP-dependent assembly of double hexamers of SV40 T antigen at the viral origin of DNA replication. Nature (London) 338:658-662.
35. McVey, D., L. Brizuela, I. Mohr, D. R. Marshak, Y. Gluzman, and D. Beach. 1989. Phosphorylation of large tumour antigen by cdc2 stimulates SV40 DNA replication. Nature (London) 341:503-507.
36. McVey, D., S. Ray, Y. Gluzman, L. Berger, A. G. Wildeman, D. R. Marshak, and P. Tegtmeyer. 1993. cdc2 phosphorylation of threonine 124 activates the origin-unwinding functions of simian virus 40 T antigen. J. Virol. 67:5206-5215.
37. McVey, D., B. Woelker, and P. Tegtmeyer. 1996. Mechanism of simian virus 40 T-antigen activation by phosphorylation of threonine 124. J. Virol. 70: 3887-3893.
38. Melendy, T., and B. Stillman. 1993. An interaction between replication protein A and SV40 T antigen appears essential for primosome assembly during SV40 DNA replication. J. Biol. Chem. 268:3389-3395.
39. Moarefi, I. F., D. Small, I. Gilbert, M. Hopfner, S. K. Randall, C. Schneider, A. A. R. Russo, U. Ramsperger, A. Arthur, H. Stahl, T. J. Kelly, and E. Fanning. 1993. Mutation of the cyclin-dependent kinase phosphorylation site in simian virus 40 (SV40) large T antigen specifically blocks SV40 origin DNA unwinding. J. Virol. 67:4992-5002.
40. Mohr, I. J., B. Stillman, and Y. Gluzman. 1987. Regulation of SV40 DNA replication by phosphorylation of T antigen. EMBO J. 6:153-160.
41. Murakami, Y., and J. Hurwitz. 1993. DNA polymerase alpha stimulates the ATP-dependent binding of simian virus tumor T antigen to the SV40 origin of replication. J. Biol. Chem. 268:11018-11027.
42. Nethanel, T., and G. Kaufmann. 1990. Two DNA polymerases may be required for synthesis of the lagging DNA strand of simian virus 40. J. Virol. 64:5912-5918.
43. Parsons, R., M. E. Anderson, and P. Tegtmeyer. 1990. Three domains in the simian virus 40 core origin orchestrate the binding, melting, and DNA helicase activities of T antigen. J. Virol. 64:509-518.
44. Parsons, R. E., J. E. Stenger, S. Ray, R. Welker, M. E. Anderson, and P. Tegtmeyer. 1991. Cooperative assembly of simian virus 40 T-antigen hexamers on functional halves of the replication origin. J. Virol. 65:2798-2806.
45. Roberts, J. 1989. Simian virus 40 (SV40) large tumor antigen causes stepwise changes in SV40 origin structure during initiation of DNA replication. Proc. Natl. Acad. Sci. USA 86:3939-3943.
46. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd. ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
47. Scheffner, M., R. Wessel, and H. Stahl. 1989. Sequence independent duplex DNA opening reaction catalyzed by SV40 large T antigen. Nucleic Acids Res. 17:93-106.
48. Schneider, J., and E. Fanning. 1988. Mutations in the phosphorylation sites of simian virus 40 (SV40) T antigen alter its origin DNA-binding specificity for sites I or II and affect SV40 DNA replication activity. J. Virol. 62:1598-1605.
49. SenGupta, D. J., and J. A. Borowiec. 1994. Strand and face: the topography of interactions between the SV40 origin of replication and T-antigen during the initiation of replication. EMBO J. 13:982-992.
50. Simmons, D. T. 1986. DNA-binding region of the simian virus 40 tumor antigen. J. Virol. 57:776-785.
51. Simmons, D. T., W. Chou, and K. Rodgers. 1986. Phosphorylation down-regulates the DNA-binding activity of simian virus 40 T antigen. J. Virol. 60:888-894.
52. Simmons, D. T., G. Loeber, and P. Tegtmeyer. 1990. Four major sequence elements of simian virus 40 large T antigen coordinate its specific and nonspecific DNA binding. J. Virol. 64:1973-1983.
53. Simmons, D. T., R. Upson, K. Wun-Kim, and W. Young. 1993. Biochemical analysis of mutants with changes in the DNA-binding domain of simian virus 40 tumor antigen. J. Virol. 67:4227-4236.
54. Simmons, D. T., K. Wun-Kim, and W. Young. 1990. Identification of simian virus 40 T antigen residues important for specific and nonspecific binding to DNA and for helicase activity. J. Virol. 64:4858-4865.
55. Stahl, H., P. Droge, and R. Knippers. 1986. DNA helicase activity of SV40 large tumor antigen. EMBO J. 5:1939-1944.
56. Stahl, H., M. Scheffner, M. Wiekowski, and R. Knippers. 1988. DNA unwinding function of the SV40 large tumor antigen. Cancer Cells 6:105-112.
57. Stillman, B. W., and Y. Gluzman. 1985. Replication and supercoiling of simian virus 40 DNA in cell extracts from human cells. Mol. Cell. Biol. 5:2051-2060.
58. Strauss, M., P. Argani, I. J. Mohr, and Y. Gluzman. 1987. Studies on the origin-specific DNA-binding domain of simian virus 40 large T antigen. J. Virol. 61:3326-3330.
59. Tsurimoto, T., M. P. Fairman, and B. Stillman. 1989. Simian virus 40 DNA replication in vitro: identification of multiple stages of initiation. Mol. Cell. Biol. 9:3839-3849.
60. Tsurimoto, T., and B. Stillman. 1991. Replication factors required for SV40 DNA replication in vitro. II. Switching of DNA polymerase α and δ during initiation of leading and lagging strand synthesis. J. Biol. Chem. 266:1961-1968.
61. Virshup, D. M., A. A. Russo, and T. J. Kelly. 1992. Mechanism of activation of simian virus 40 DNA replication by protein phosphatase 2A. Mol. Cell. Biol. 12:4883-4895.
62. Waga, S., and B. Stillman. 1994. Anatomy of a DNA replication fork revealed by reconstitution of SV40 DNA replication in vitro. Nature (London) 369:207-212.
63. Weinberg, D. H., K. L. Collins, P. Simancek, A. Russo, M. S. Wold, D. M. Virshup, and T. J. Kelly. 1990. Reconstitution of simian virus 40 DNA replication with purified proteins. Proc. Natl. Acad. Sci. USA 87:8692-8696.
64. Wessel, R., J. Schweizer, and H. Stahl. 1992. Simian virus 40 T-antigen DNA helicase is a hexamer which forms a binary complex during bidirectional unwinding from the viral origin of DNA replication. J. Virol. 66:804-815.
65. Wiekowski, M., P. Dröge, and H. Stahl. 1987. Monoclonal antibodies as probes for function of large T antigen during the elongation process of simian virus 40 DNA replication. J. Virol. 61:411-418.
66. Wiekowski, M., M. W. Schwarz, and H. Stahl. 1988. Simian virus 40 large T antigen DNA helicase. Characterization of the ATPase-dependent DNA unwinding activity and its substrate requirements. J. Biol. Chem. 263:436-442.
67. Wobbe, C. R., L. Weissbach, J. H. Borowiec, F. Dean, and J. Hurwitz. 1985. In vitro replication of duplex circular DNA containing the simian virus 40 DNA origin site. Proc. Natl. Acad. Sci. USA 82:5710-5714.
68. Wold, M. S., J. J. Li, and T. J. Kelly. 1987. Initiation of simian virus 40 DNA replication in vitro: large-tumor-antigen-and origin-dependent unwinding of the template. Proc. Natl. Acad. Sci. USA 84:3643-3647.
69. Wun-Kim, K., and D. T. Simmons. 1990. Mapping of helicase and helicase substrate binding domains on simian virus 40 large T antigen. J. Virol. 64:2014-2020.
70. Wun-Kim, K., R. H. Upson, W. Young, and D. T. Simmons. 1993. The DNA binding domain of simian virus 40 large T antigen has multiple functions. J. Virol. 67:7608-7611.