Quantitative analyses of myosin heavy-chain mRNA and protein isoforms in single fibers reveal a pronounced fiber heterogeneity in normal rabbit muscles

European Journal of Biochemistry

Volumn 247, Issue 1, 1997, Pages 30-36

  Peuker, Heidemarie ^a   Pette, Dirk ^a  

^a UNIVERSITY OF KONSTANZ   (Germany)

Author keywords

Coexpression; Direct reverse transcriptase PCR; Muscle fiber type; Myosin heavy chain isoform; Single fiber analysis

Indexed keywords

MESSENGER RNA; MYOSIN HEAVY CHAIN;

ANIMAL TISSUE; ARTICLE; DNA SEQUENCE; IMMUNOHISTOCHEMISTRY; LEG MUSCLE; MUSCLE CELL; NONHUMAN; PRIORITY JOURNAL; PROTEIN DETERMINATION; RABBIT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

ANIMALIA; ORYCTOLAGUS CUNICULUS;

EID: 0030858404     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.1997.00030.x     Document Type: Article

References (38)

1. Hämäläinen, N. & Pette, D. (1993) The histochemical profiles of fast fiber types IIB. IID and IIA in skeletal muscles of mouse, rat and rabbit, J. Histochem. Cytochem. 41, 733-743.
2. Gorza, L. (1990) Identification of a novel type 2 fiber population in mammalian skeletal muscle by combined use of histochemical myosin ATPase and anti-myosin monoclonal antibodies, J. Histochem. Cytochem. 38, 257-265.
3. Sehiaffino, S., Gorza, L., Sartore, S., Saggin, L., Ausoni, S., Vianello, M., Gundersen, K. & Lömo, T. (1989) Three myosin heavy chain isoforms in type 2 skeletal muscle fibres. J. Muscle Res. Cell Motil. 10, 197-205.
4. Termin, A., Staron, R. S. & Pette, D. (1989) Myosin heavy chain isoforms in histochemically defined fiber types of rat muscle. Histochemistry 92, 453-457.
5. Aigner, S., Gohlsch, B., Hämäläinen, N., Staron, R. S., Uber, A., Wehrle, U. & Pette, D. (1993) Fast myosin heavy chain diversity in skeletal muscles of the rabbit: heavy chain IId, not IIb predominates. Eur. J. Biochem. 211, 367-372.
6. Pette, D. & Staron, R. S. (1990) Cellular and molecular diversities of mammalian skeletal muscle fibers, Rev. Physiol. Biochem. Pharmacol. 116, 1-76.
7. Sehiaffino, S. & Reggiani, C. (1994) Myosin isoforms in mammalian skeletal muscle. J. Appl. Physiol. 77, 493-501.
8. LaFramboise, W. A., Daood, M. J., Guthrie, R. D., Moretti, P., Schiaffino, S. & Ontell, M (1990) Electrophoretic separation and immunological identification of type 2X myosin heavy chain in rat skeletal muscle, Biochem. Biophys. Acta 1035, 109-112.
9. Lompré. A.-M., Nadal-Ginard, B. & Mahdavi, V. (1984) Expression of the cardiac ventricular a- and b-myosin heavy chain genes is developmentally and hormonally regulated. J. Biol. Chem. 259, 6437-6446.
10. Peuker, H. & Pette, D. (1995) Reverse transcriptase-polymerase chain reaction detects induction of cardiac-like α myosin heavy chain mRNA in low frequency stimulated rabbit fast-twitch muscle, FEBS Lett. 367, 132-136.
11. Hämäläinen, N. & Pette, D. (1997) Expression of an α-cardiac like myosin heavy chain in diaphragm, chronically stimulated, and denervated fast-twitch muscles of rabbit. Muscle Res. Cell Motil., in the press.
12. Uber, A. & Pette, D. (1993) PCR-based assignment of two myosin heavy chain cDNA clones to biochemically and histochemically defined single type IIB and IID fibers of rabbit muscle, FEBS Lett. 331, 193-197.
13. Peuker, H. & Pette, D. (1995) Direct reverse transcriptase-polymerase chain reaction for determining specific mRNA expression levels in muscle fiber fragments. Anal. Biochem. 224, 443-446.
14. Staron, R. S. & Pette, D. (1986) Correlation between myofibrillar ATPase activity and myosin heavy chain composition in rabbit muscle fibers, Histochemistry 86, 19-23.
15. Maeda, K., Sczakiel, G. & Wittinghofer, A. (1987) Characterization of a cDNA coding for the complete light meromyosin portion of a rabbit fast skeletal muscle myosin heavy chain. Eur. J. Biochem. 167, 97-102.
16. Sinha, A. M., Umeda, P. K., Kavinsky, C. J., Rajamanickam, C., Hsu, H.-J., Jakovcic, S. & Rabinowitz, M. (1982) Molecular cloning of mRNA sequences for cardiac α- and β-form myosin heavy chains: expression in ventricles of normal. hypothyroid, and thyrotoxic rabbits. Proc. Natl Acad. Sci. USA 79, 5847-5851.
17. Matschinsky, F. M., Passonneau, J. V. & Lowry, O. H. (1968) Quantitative histochemical analysis of glycolytic intermediates and cofactors with an oil well technique. J. Histochem. Cytochem. 16, 29-39.
18. Lowry, O. H. & Passonneau, J. V. (1972) A flexible system of enzymatic analysis, Academic Press, New York, London.
19. Fink, H. & Pette, D. (1983) An automated micropipet especially designed for use with the oil-well technique. Anal. Biochem. 133, 220-225.
20. Bassam, B, J., Caetano-Anollés, G. & Gresshof, P. M. (1991) Fast and sensitive silver staining of DNA in polyacrylamide gels. Anal. Biochem. 196, 80-83.
21. Peuker, H. & Pette, D. (1993) Nonradioactive reverse transeriptase/ polymerase chain reaction for quantification of myosin heavy chain mRNA isoforms in various rabbit muscles, FEBS Lett. 318, 253-258.
22. Termin, A., Staron, R. S. & Pette, D. (1989) Changes in myosin heavy chain isoforms during chronic low-frequency stimulation of rat fast hindlimb muscles: a single fiber study, Eur. J. Biochem. 186, 749-754.
23. Dynal (1992) Technical handbook of molecular biology, 1st edn. Dynal AS, Oslo.
24. Allen, D. L., Yasui, W., Tanaka, T., Ohira, Y., Nagaoka, S., Sekiguchi, C., Hinds, W. E., Roy, R. R. & Edgerton, V. R. (1996) Myonuclear number and myosin heavy chain expression in rat soleus single muscle fibers after spaceflight, J. Appl. Physiol. 81, 145-151.
25. Staron, R. S. & Pette, D. (1993) The continuum of pure and hybrid myosin heavy chain-based fiber types in rat skeletal muscle. Histochemistry 100, 149-153.
26. Zardini, D. M. & Parry, D, J. (1994) Identification, distribution, and myosin subunit composition of type IIX fibers in mouse muscles, Muscle Nerve 17, 1308-1316.
27. Bottinelli, R., Betto, R., Schiaffino, S. & Reggiani, C. (1994) Maximum shortening velocity and coexistence of myosin heavy chain isoforms in single skinned fast fibres of rat skeletal muscle, J. Muscle Res. Cell Motil. 15, 413-419.
28. DeNardi, C., Ausoni, S., Moretti, P., Gorza, L., Velleca, M., Buckingham. M. & Schiaffino, S. (1993) Type-2X-myosin heavy chain is coded by a muscle fiber type-specific and developmentally regulated gene, J. Cell Biol. 123, 823-835.
29. Staron, R. S. & Pette, D. (1987) The multiplicity of myosin light and heavy chain combinations in histochemically typed single fibres. Rabbit soleus muscle, Biochem. J. 243, 687-693.
30. Staron, R. S. & Johnson, P. (1993) Myosin polymorphism and differential expression in adult human skeletal muscle. Comp. Biochem. Physiol. B Comp. Biochem. 106, 463-475.
31. Smerdu, V., Karsch-Mizrachi, I., Campione, M., Leinwand, L. & Schiaffino, S. (1994) Type IIx myosin heavy chain transcripts are expressed in type IIb fibers of human skeletal muscle. Am. J. Physiol. 36, 1723-1728.
32. Ennion, S., Sant'ana Pereira, J., Sargeant, A. J., Young, A. & Goldspink, G. (1995) Characterization of human skeletal muscle fibres according to the myosin heavy chains they express. J. Muscle Res. Cell Motil. 16, 35-43.
33. Pette, D. & Staron, R. S. (1993) The molecular diversity of mammalian muscle fibers, News Physiol. Sci. 8, 153-157.
34. Galler, S., Schmitt, T. & Pette, D. (1994) Stretch activation. unloaded shortening velocity, and myosin heavy chain isoforms of rat skeletal muscle fibres, J. Physiol. (Lond.) 478, 523-531.
35. Staron, R. S. & Pette, D. (1987) Nonuniform myosin expression along single fibers of chronically stimulated and contralateral rabbit tibialis anterior muscles, Pflügers Arch, Eur. J. Physiol. 409, 67-73.
36. Wehrle, U., Düsterhöft, S. & Pette, D. (1994) Effects of chronic electrical stimulation on myosin heavy chain expression in satellite cell cultures derived from rat muscles of different fiber-type composition. Differentiation 58, 37-46.
37. Sindhwani, R., Ismail-Beigi, F. & Leinwand, L. A. (1994) Posttranscriptional regulation of rat α cardiac myosin heavy chain gene expression, J. Biol. Chem. 269, 3272-3276.
38. Babij, P. & Periasamy, M. (1989) Myosin heavy chain isoform diversity in smooth muscle is produced by differential RNA splicing, J. Mol. Biol. 210, 673-679.