Site-directed mutagenesis of a fungal β-1,4-endoglucanase increases the minimum size required for the substrate

Applied Microbiology and Biotechnology

Volumn 48, Issue 2, 1997, Pages 225-231

  Wang, H ^a   Jones, R W ^a  

^a PURDUE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BETA 1,4 ENDOGLUCANASE; CELLOBIOSE; GLUCAN SYNTHASE; UNCLASSIFIED DRUG;

ARTICLE; ENZYME BINDING; ENZYME SPECIFICITY; ENZYME STRUCTURE; ENZYME SUBSTRATE COMPLEX; ESCHERICHIA COLI; FUNGUS; MACROPHOMINA PHASEOLINA; NONHUMAN; SITE DIRECTED MUTAGENESIS;

AMINO ACID SEQUENCE; BASE SEQUENCE; BLOTTING, WESTERN; CELLULASE; CELLULOSE; MITOSPORIC FUNGI; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; SUBSTRATE SPECIFICITY;

ESCHERICHIA COLI; FUNGI; MACROPHOMINA PHASEOLINA;

EID: 0030858002     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002530051042     Document Type: Article

References (34)

1. Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215: 403-410
2. Alzari PM, Souchon H, Dominguez R (1996) The crystal structure of endoglucanase CelA. a family 8 glycosyl hydrolase from Clostridium thermacellum. Structure 4: 265-275
3. Baird SD, Hefford MA, Johnson DA, Sung WL, Yaguchi M, Seligy VL (1990) The Glu residue in the conserved Asn-Glu-Pro sequence of two highly divergent endo-β-1,4-glucanases is essential for enzymatic activity. Biochem Biophys Res Commun 169: 1035-1039
4. Barnelt C, Sumner L, Berka R, Shoemaker S, Berg HG, Gritzali M, Brown R (1993) Properties of native and site-mutagenized cellobiohydrolase II. In: Himmel ME, Georgiou G (eds) Biocatalyst design for stability and specificity. American Chemical Society, NY, pp 220-232
5. Belaich A, Fierobe H-P, Baty D, Busetta B, Bagnara-Tardif C, Gaudin C, Belaich J-P (1992) The catalytic domain of endoglucanase A from Clostridium cellulolyticum: effects of arginine 79 and histidine 122 mutations on catalysis. J Bacteriol 174: 4677-4682
6. Bortoli-German I, Haiech J, Chippaux M, Barras F (1995) Informational suppression to investigate structural functional and evolutionary aspects of the Erwinia chrysanthemi cellulase EGZ. J Mol Biol 246: 82-94
7. Carpita NC, Gibeaut DM (1993) Structural models of primary cell walls in flowering plants: consistency of molecular structure with the physical properties of the wall during growth. Plant J 3: 1-30
8. Chauvaux S, Beguin P, Aubert J-P (1992) Site-directed mutagenesis of essential carboxylic residues in Clostridium thermocellum endoglucanase CelD. J Biol Chem 267: 4472-4478
9. Davies G, Henrissat B (1995) Structures and mechanisms of glycosyl hydrolases. Structure 3: 853-859
10. Davies G, Dodson G, Hubbard R, Tolley S, Dauter Z, Wilson K, Hjort C, Mikkelsen J, Rasmussen G, Schulein M (1993) Structure and function of endoglucanase V. Nature 365: 362-364
11. Derewenda U, Swenson L, Green R, Wei Y, Morosoli R, Shareck F, Kluepfel D, Derewenda ZS (1994) Crystal structure, at the 2.6-A resolution, of the Streptomyces lividans xylanase A, a member of the F family of β-1,4-D-glycanases. J Biol Chem 269: 20 811-20 814
12. Dominguez R, Souchon H, Spinelli S, Dauter Z, Wilson KS, Chauvaux S, Beguin P, Alzari PM (1995) A commun protein fold and similar active site in two distinct families of β-glycanases. Nature Struct Biol 2: 569-576
13. Ducros V, Czjzek M, Belaich A, Gaudin C, Fierobe H-P, Belaich J-P, Davies GJ, Haser R (1995) Crystal structure of the catalytic domain of a bacterial cellulase belonging to family 5. Structure 15: 939-949
14. Gilkes NR, Henrissat B, Kilburn DG, Miller RC, Warren RAJ (1991) Domains in microbial β-1,4 glycanases: sequence conservation, function, and enzyme families. Microbiol Rev 55: 303-315
15. Harris GW, Jenkins JA, Connerton I, Cummings N, LoLeggio L, Scott M, Hazelwood G, Laurie JI, Gilbert HJ, Pickersgill RW (1994) Structure of the catalytic core of the family F xylanase from Pseudomonas flourescens and identification of the xylopentaose-binding sites. Structure 2: 1107-1116
16. Henrissat B (1993) Hidden domains and active site residues in β-glycanase-encoding gene sequences? Gene 125: 199-204
17. Henrissat B, Claeyssens M, Tomme P, Lemesle L, Mornon J-P (1989) Cellulase families revealed by hydrophobic cluster analysis. Gene 81: 83-95
18. Hoffren A-M, Teeri TT, Teleman O (1995) Molecular dynamics simulation of fungal cellulose-binding domains: differences in molecular rigidity but a preserved cellulose binding surface. Protein Eng 8: 443-450
19. Juncosa-Ginesta M, Pons J, Planas A, Querol E (1994) Improved efficiency in site-directed mutagenesis by PCR using a Pyrococcus sp. GB-D polymerase. Biotechniques 16: 820-824
20. Linder M, Mattinen M-L, Kontteli M, Lindeberg G, Stahlberg J, Drakenberg T, Reinikainen T, Pettersson G, Annila A (1995) Identification of functionally important amino acids in the cellulose-binding domain of Trichoderma reesei cellobiohydrolase I. Protein Sci 4: 1056-1064
21. Macarron R, Acebal C, Castillon P, Dominguez JM, Mata I, Pettersson G, Tomme P, Claeyssens M (1993a) Mode of action of endoglucanase III from Trichoderma reesei. Biochem J 289: 867-873
22. Macarron R, Beeumen J van, Henrissat B, Mata I, Claeyssens M (1993b) Identification of an essential glutamate residue in the active site of endoglucanase III from Trichoderma reesei. FEBS Lett 316: 137-140
23. Moreau A, Shareck F, Kluepfel D, Morosoli R (1994) Alteration of the cleavage mode and of the transglycosylation reactions of the xylanase A of Streptomyces lividans 1326 by site-directed mutagenesis of the Asn173 residue. Eur J Biochem 219: 261-266
24. Navas J, Beguin P (1992) Site-directed mutagenesis of conserved residues of Clostridium thermocellum endoglucanase Ce1C. Biochem Biophys Res Commun 189: 807-812
25. Py B, Bortoli-German I, Haiech J, Chippaux M, Barras F (1991) Cellulase EGZ of Erwinia chrysanthemi: structural organization and importance of His98 and Glu133 residues for catalysis. Protein Eng 3: 325-333
26. Reinikainen T, Ruohonen L, Nevanen T, Laaksonen L, Kraulis P, Jones TA, Knowles JKC, Teeri TT (1992) Investigation of the function of mutated cellulose-binding domains of Trichoderma reesi cellobiohydrolase I. Proteins 14: 475-482
27. Schmid G (1988) Preparation of cellodextrins: another perspective. Methods Enzymol 160: 38-45
28. Schou C, Rasmussen G, Kaltoft M, Henrissat B, Schulein M (1993) Stereochemistry, specificity and kinetics of the hydrolysis of reduced cellodextrins by nine cellulases. Eur J Biochem 217: 947-953
29. Taylor JS, Teo B, Wilson DB, Brady JW (1995) Conformation modeling of substrate binding to endocellulase E2 from Thermomonospora fusca. Protein Eng 7: 1145-1152
30. Vyas N (1991) Atomic features of protein-carbohydrate interactions. Curr Opin Struct Biol 1: 732-740
31. Wang H, Jones RW (1995a) A unique endoglucanase-encoding gene cloned from the phytopathogenic fungus Macrophomina phaseolina. Appl Environ Microbiol 61: 2004-2006
32. Wang H, Jones RW (1995b) Cloning, characterization and functional expression of an endoglucanase-encoding gene from the phytopathogenic fungus Macrophomina phaseolina. Gene 158: 125-128
33. 280 is the nucleophile in the active site of Clostridium thermocellum Ce1C, a family A endo-β-1,4-glucanase. J Biol Chem 268: 14096-14102
34. White A, Withers SG, Gilkes NR, Rose DR (1994) Crystal structure of the catalytic domain of the β-1,4 glycanase Cex from Cellulomonas fimi. Biochemistry 33: 12 546-12 552