The effect of N-linked glycosylation on molecular weight, thrombin cleavage, and functional activity of human protein S

Thrombosis and Haemostasis

Volumn 77, Issue 6, 1997, Pages 1156-1163

  Lu, Deshun ^a,b   Xie, Rong Lin ^a   Rydzewski, Andrzej ^a,c   Long, George L ^a  

^a UNIVERSITY OF VERMONT   (United States)

^b WASHINGTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c MEDICAL UNIVERSITY OF BIALYSTOK   (Poland)

Author keywords

[No Author keywords available]

Indexed keywords

BLOOD CLOTTING FACTOR 5A; CARBOHYDRATE; COMPLEMENT COMPONENT C4B; COMPLEMENTARY DNA; GLUCAN SYNTHASE; PROTEIN S; RECOMBINANT PROTEIN; THROMBIN;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; CONSENSUS SEQUENCE; CONTROLLED STUDY; DNA TRANSFECTION; EXPRESSION VECTOR; KIDNEY CELL; MASS SPECTROMETRY; MOLECULAR WEIGHT; MUTAGENESIS; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN GLYCOSYLATION; PROTEIN PURIFICATION; SITE DIRECTED MUTAGENESIS;

EID: 0030857908     PISSN: 03406245     EISSN: None     Source Type: Journal    
DOI: 10.1055/s-0038-1656130     Document Type: Article

References (46)

1. Di Scipio RG, Davie EW. Characterization of protein S, a γ-carboxy-glutamic acid containing protein from bovine and human plasma. Biochemistry 1979; 18: 899-904.
2. Walker FJ. Protein S and the regulation of activated protein C. Semin in Thromb and Hemost 1984; 10: 131-8.
3. Kisiel W, Canfield WM, Ericsson LH, Davie EW. Anticoagulant properties of bovine plasma protein C following activation by thrombin. Biochemistry 1977; 16: 5824-31.
4. Marlar RA, Kleiss AJ, Griffin JH. Mechanism of action of human activated protein C, a thrombin-dependent anticoagulant enzyme. Blood 1982; 59: 1067-72.
5. Walker FJ. The regulation of activated protein C by a new protein: A possible function for bovine protein S. J Biol Chem 1980; 255: 5521-4.
6. Walker FJ, Chavin SI, Fay PJ. Inactivation of factor VIII by activated protein C and protein S. Arch Biochem Biophys 1987; 252: 322-8.
7. Solymoss S, Tucker MM, Tracy PB. Kinetics of inactivation of membrane-bound factor Va by activated protein C. J Biol Chem 1988; 263: 14884-90.
8. R506Q by activated protein C. J Biol Chem 1995; 270: 27852-8.
9. Heeb MJ, Mesters RM, Tans G, Rosing J, Griffin JH. Binding of protein S to factor Va associated with inhibition of prothrombinase that is independent of activated protein C. J Biol Chem 1993; 268: 2872-7.
10. Heeb MJ, Rosing J, Bakker HM, Fernandez JA, Tans G, Griffin JH. Protein S binds to and inhibits factor Xa. Proc Natl Acad Sci USA 1994; 91: 2728-32.
11. Comp PC, Esmon CT. Recurrent venous thromboembolism in patients with a partial deficiency of protein S. N Engl J Med 1984; 311: 1525-8.
12. Comp PC, Nixon RR, Cooper MR, Esmon CT. Familial protein S deficiency is associated with recurrent thrombosis. J Clin Invest 1984; 74: 2082-8.
13. Schmidel DK, Nelson RM, Broxson EH, Comp PC, Marlar RA, Long GL. A 5.3-kb deletion including exon XIII of the protein Sa gene occurs in two protein S deficient families. Blood 1991; 77: 551-9.
14. Fair DS, Marlar RA. Biosynthesis and secretion of factor VII, protein C, protein S, and the protein C inhibitor from a human hepatoma cell line. Blood 1986; 67: 64-70.
15. Fair DS, Marlar RA, Levin EG. Human endothelial cells sythesize protein S. Blood 1986; 67:1 168-71.
16. Malm J, He XH, Bjartell A, Shen L, Abrahamsson P-A, Dahlbäck B. Vitamin K-dependent protein S in Leydig cells of human testis. Biochem J 1994; 302: 845-50.
17. Lundwall A, Dackowski W, Cohen E, Shaffer M, Mahr A, Dahlbäck B, Stenflo J, Wydro R. Isolation and sequence of the cDNA for human protein S, a regulator of blood coagulation. Proc Natl Acad Sci USA 1986; 83: 6716-20.
18. Hoskins J, Norman DK, Beckmann RJ, Long, GL. Cloning and characterization of human liver cDNA encoding a protein S precursor. Proc Natl Acad Sci USA 1987; 84: 349-53.
19. Gershagen S, Lundwell A, Fernlund P. Characterization of the human sex hormone binding globulin (SHBG) gene and demonstration of two transcripts in both liver and testis. Nuc Acids Res 1989; 17: 9245-58.
20. Walker, FJ. Regulation of vitamin K-dependent protein S: Inactivation by thrombin. J Biol Chem 1984; 259: 10335-9.
21. Dahlbäck B, Lundwall A, Stenflo J. Localization of thrombin cleavage sites in the amino-terminal region of bovine protein S. J Biol Chem 1986; 261: 5111-5.
22. Chang GTG, Aaldering L, Hackeng TM, Reitsma PH, Bertina RM, Bouma BN. Construction and characterization of thrombin-resistant variants of recombinant human protein S. Thromb Haemost 1994; 72: 693-7.
23. Dahlbäck B, Stenflo J. High molecular weight complex in human plasma between vitamin K-dependent protein S and complement component C4b-binding protein. Proc Natl Acad Sci USA 1981; 78: 2512-6.
24. Schalbe R, Dahlbäck B, Hillarp A, Nelsestuen G. Assembly of protein S and C4b-binding protein on membranes. J Biol Chem 1990; 265: 16074-81.
25. Griffin JH, Gruber A, Fernandez JA. Reevaluation of total, free, and bound protein S and C4b-binding protein levels in plasma anticoagulated with citrate or hirudin. Blood 1992; 79: 3203-11.
26. Nelson RM, Long GL. Solution-phase equilibrium binding interaction of human protein S with C4b-binding protein. Biochemistry 1991; 30: 2384-90.
27. Weinstein RE, Walker FJ. Enhancement of rabbit protein S anticoagulant cofactor activity in vivo by modulation of the protein S C4B binding protein interaction. J Clin Invest 1990; 86: 1928-35.
28. Fernandez JA, Heeb MJ, Griffin JH. Identification of residues 413-433 of plasma protein S as essential for binding to C4b-binding protein. J Biol Chem 1993; 268: 16788-94.
29. Greengard JS, Fernandez JA, Radtke K-P, Griffin JH. Identification of candidate residues for interaction of protein S with C4b binding protein and activated protein C. Biochem J 1995; 305: 397-403.
30. Nelson RM, Long GL. Binding of protein S to C4b-binding protein: Mutagenesis of protein S. J Biol Chem 1992; 267: 8140-5.
31. Schmidel DK, Tatro AV, Phelps LG, Tomczak JA, Long GL. Organization of the human protein S gene. Biochemistry 1990; 29:7845-52.
32. 34 → Met). J Biol Chem 1994; 269: 29032-8.
33. Dahlbäck B. Purification of human C4b-binding protein and formation of its complex with vitamin K-dependent protein S. Biochem J 1983; 209: 847-56.
34. Foster DC, Rudinski MS, Schach BG, Berkner KL, Kumar AA, Hagen FS, Sprechr CA, Insley MY, Davie EW. Propeptide of human protein C is necessary for γ-carboxylation. Biochemistry 1987; 26: 7003-11.
35. Nelson RM, Long GL. A general method of site-specific mutagenesis using a modification of the Taq polymerase chain reaction. Anal Biochem 1989; 180: 147-51.
36. Graham FL, Van der Eb AJ. A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology 1973; 52: 456-67.
37. Kuwada M, Katayama K. A high-performance liquid chromatographic method for the simultaneous determination of γ-carboxyglutamic acid and glutamic acid in proteins, bone, and urine. Anal Biochem 1981; 117: 259-65.
38. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970; 227: 680-5.
39. Kalafatis M, Mann KG. Role of the membrane in the inactivation of factor Va by activated protein C. J Biol Chem 1993; 268: 27246-57.
40. Langdell RD, Wagner RH, Brinkhous KM. Effect of antihemophilic factor on one-stage clotting tests. J Lab Clin Med 1953; 41: 637-47.
41. Krishnaswamy S, Williams EB, Mann KG. The binding of activated protein C to factors V and Va. J Biol Chem 1986; 261: 9684-93.
42. Kornfeld R, Kornfeld S. Assembly of asparagine-linked oligosaccharides. Ann Rev Biochem 1985; 54:631-64.
43. Rademacher TW, Parekh RB, Dwek RA. Glycobiology. Ann Rev Biochem 1988; 57: 785-838.
44. 460 to Pro substitution of the protein Sα (PROS1) gene is a frequent mutation associated with free protein S (type IIa) deficiency. Blood 1995; 86: 3436-43.
45. Bertina RM, Ploos van Amstel HK, van Wijingaarden A, Coenen J, Leemhius MP, Deutz-Terlouw PP, van der Linden IK, and Reitsma PH. Heerlen polymorphism of protein S, an immunologic polymorphism due to dimorphism of residue 460. Blood 1990; 76: 538-48.
46. Horrevoets AJG, Smilde AE, Fredenburgh JC, Pannekoek H, Nesheim ME. The activation-resistant conformation of recombinant human plasminogen is stabilized by basic residues in the amino-terminal hinge region. J Biol Chem 1995; 270: 15770-6.