Isolation of Bacillus megaterium mutants that produce high levels of heterologous protein, and their use to construct a highly mosquitocidal strain

Current Microbiology

Volumn 35, Issue 2, 1997, Pages 71-76

  England, Dacre F ^a   Penfold, Robert J ^a   Delaney, Stephen F ^a   Rogers, Peter L ^a  

^a UNIVERSITY OF NEW SOUTH WALES   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; BACTERIAL TOXIN;

ARTICLE; BACILLUS MEGATERIUM; BACTERIUM MUTANT; ESCHERICHIA COLI; MOSQUITO; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; TOXIN ANALYSIS;

ANIMALS; BACILLUS MEGATERIUM; BACTERIAL TOXINS; BIOLOGICAL ASSAY; CLONING, MOLECULAR; CULEX; DOSE-RESPONSE RELATIONSHIP, DRUG; LARVA; MUTAGENESIS; PLASMIDS; RECOMBINANT PROTEINS; ULTRAVIOLET RAYS;

BACILLUS MEGATERIUM; BACILLUS SPHAERICUS; BACTERIA (MICROORGANISMS); CULEX PIPIENS QUINQUEFASCIATUS; ESCHERICHIA COLI;

EID: 0030857155     PISSN: 03438651     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002849900215     Document Type: Article

References (24)

1. Baumann P, Clark MA, Baumann L, Broadwell AH (1991) Bacillus sphaericus as a mosquito pathogen: properties of the organism and its toxin. Microbiol Rev 55:425-436
2. Bourgouin C, Delecluse A, de la Torre F, Szulmajster J (1990) Transfer of the toxin protein genes of Bacillus sphaericus into Bacillus thuringiensis subsp. israelensis and their expression. Appl Environ Microbiol 56:340-344
3. Broadwell AH, Baumann L, Baumann P (1990) The 42-and 51 kilodalton mosquitocidal proteins of Bacillus sphaericus 2362: construction of recombinants with enhanced expression and in vivo studies of processing and toxicity. J Bacteriol 172:2217-2223
4. Charles JF, Nicolas L (1986) Recycling of Bacillus sphaericus 2362 in mosquito larvae: a laboratory study. Ann Inst Pasteur/Microbiol 137B:101-111
5. Davidson EW, Urbina M, Payne J, Mulla MS, Darwazeh H, Dulmage HT, Correa JA (1984) Fate of Bacillus sphaericus 1593 and 2362 spores used as larvicides in the aquatic environment. Appl Environ Microbiol 47:125-129
6. Finney DJ (1971) Probit analysis. London: Cambridge University Press
7. Hoheisel JD (1994) Creation of a rare cutter multiple cloning site. BioTechniques 17:456-460
8. Kokotek W, Lotz W (1989) Construction of a lacZ-kanamycin-resistance cassette, useful for site-directed mutagenesis and as a promoter probe. Gene 84:467-471
9. Liu JW, Porter AG, Wee BY, Thanabalu T (1996a) New gene from nine Bacillus sphaericus strains encoding highly conserved 35.8-kilodalton mosquitocidal toxins. Appl Environ Microbiol 62:2174-2176
10. Liu JW, Yap WH, Thanabalu T, Porter AG (1996b) Efficient synthesis of mosquitocidal toxins in Asticcacaulis excentricus demonstrates potential of Gram-negative bacteria in mosquito control. Nature Biotechnol 14:343-347
11. Meinhardt F, Stahl U, Ebeling W (1989) Highly efficient expression of homologous and heterologous genes in Bacillus megaterium. Appl Microbiol Biotechnol 30:343-350
12. Osborne KJ (1995) Growth, sporulation and larvicidal activity of the mosquito pathogen Bacillus sphaericus 2362 grown in complex and defined media. PhD Thesis, Department of Biotechnology, University of New South Wales, Sydney, Australia
13. Porter AG (1996) Mosquitocidal toxins, genes and bacteria: the hit squad. Parisitol Today 12:175-179
14. Porter AG, Davidson EW, Liu JW (1993) Mosquitocidal toxins of Bacilli and their genetic manipulation for effective biological control of mosquitoes. Microbiol Rev 57:838-861
15. Priest FG (1992) Biological control of mosquitoes and other biting flies by Bacillus sphaericus and Bacillus thuringiensis. J Appl Bacteriol 72:357-369
16. Rygus T, Hillen W (1991) Inducible high-level expression of heterologous genes in Bacillus megaterium using the regulatory elements of the xylose-utilization operon. Appl Microbiol Biotechnol 35:594-599
17. Rygus T, Hillen W (1992) Catabolite repression of the xyl operon in Bacillus megaterium. J Bacteriol 174:3049-3055
18. Rygus T, Scheler A, Allmansberger, Hillen W (1991) Molecular cloning, structure, promoters and regulatory elements for transcription of the Bacillus megaterium encoded regulon for xylose utilization. Arch Microbiol 155:535-542
19. Sambrook J, Frisch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press
20. Thanabalu T, Porter AG (1996) A Bacillus sphaericus gene encoding a novel type of mosquitocidal toxin of 31.8 kDa. Gene 170:85-89
21. Thanabalu T, Hindley J, Jackson-Yap J, Berry C (1991) Cloning, sequencing, and expression of a gene encoding a 100-kilodalton mosquitocidal toxin from Bacillus sphaericus SSII-1. J Bacteriol 173:2776-2785
22. Vary PS (1994) Prime time for Bacillus megaterium. Microbiology (Reading) 140:1001-1013
23. Von Tersch MA, Robbins HL (1990) Efficient cloning in Bacillus megaterium: comparison to Bacillus subtilis and Escherichia coli cloning hosts. FEMS Microbiol Lett 70:305-310
24. Vorobjeva IP, Khmel IA, Alfoldi L (1980) Transformation of Bacillus megaterium protoplasts by plasmid DNA. FEMS Microbiol Lett 7:261-263